Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_469

Experiment: Ying_Nucleobase14 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_468 and Pf6N2E2_469 are separated by 251 nucleotidesPf6N2E2_469 and Pf6N2E2_470 overlap by 4 nucleotidesPf6N2E2_470 and Pf6N2E2_471 overlap by 1 nucleotides Pf6N2E2_468 - DNA alkylation repair enzyme, at 534,448 to 535,551 _468 Pf6N2E2_469 - lipoprotein, putative, at 535,803 to 536,111 _469 Pf6N2E2_470 - hypothetical protein, at 536,108 to 536,431 _470 Pf6N2E2_471 - putative metalloprotease, at 536,431 to 537,492 _471 Position (kb) 535 536 537Strain fitness (log2 ratio) -1 0 1at 534.971 kb on + strand, within Pf6N2E2_468at 534.971 kb on + strand, within Pf6N2E2_468at 534.972 kb on - strand, within Pf6N2E2_468at 534.972 kb on - strand, within Pf6N2E2_468at 534.972 kb on - strand, within Pf6N2E2_468at 534.972 kb on - strand, within Pf6N2E2_468at 534.972 kb on - strand, within Pf6N2E2_468at 535.096 kb on + strand, within Pf6N2E2_468at 535.208 kb on + strand, within Pf6N2E2_468at 535.209 kb on - strand, within Pf6N2E2_468at 535.251 kb on - strand, within Pf6N2E2_468at 535.251 kb on - strand, within Pf6N2E2_468at 535.262 kb on + strand, within Pf6N2E2_468at 535.262 kb on + strand, within Pf6N2E2_468at 535.262 kb on + strand, within Pf6N2E2_468at 535.262 kb on + strand, within Pf6N2E2_468at 535.262 kb on + strand, within Pf6N2E2_468at 535.263 kb on - strand, within Pf6N2E2_468at 535.263 kb on - strand, within Pf6N2E2_468at 535.263 kb on - strand, within Pf6N2E2_468at 535.263 kb on - strand, within Pf6N2E2_468at 535.354 kb on + strand, within Pf6N2E2_468at 535.460 kb on + strandat 535.581 kb on + strandat 535.582 kb on - strandat 535.582 kb on - strandat 535.582 kb on - strandat 535.582 kb on - strandat 535.584 kb on - strandat 535.584 kb on - strandat 535.740 kb on - strandat 536.052 kb on + strand, within Pf6N2E2_469at 536.052 kb on + strand, within Pf6N2E2_469at 536.053 kb on - strand, within Pf6N2E2_469at 536.110 kb on - strandat 536.110 kb on - strandat 536.591 kb on + strandat 536.648 kb on + strand, within Pf6N2E2_471at 536.648 kb on + strand, within Pf6N2E2_471at 536.648 kb on + strand, within Pf6N2E2_471at 536.649 kb on - strand, within Pf6N2E2_471at 536.649 kb on - strand, within Pf6N2E2_471at 536.873 kb on - strand, within Pf6N2E2_471at 536.942 kb on + strand, within Pf6N2E2_471at 536.943 kb on - strand, within Pf6N2E2_471at 537.095 kb on + strand, within Pf6N2E2_471at 537.096 kb on - strand, within Pf6N2E2_471

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2
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534,971 + Pf6N2E2_468 0.47 -0.1
534,971 + Pf6N2E2_468 0.47 -0.7
534,972 - Pf6N2E2_468 0.47 +0.3
534,972 - Pf6N2E2_468 0.47 +0.9
534,972 - Pf6N2E2_468 0.47 -1.1
534,972 - Pf6N2E2_468 0.47 +0.9
534,972 - Pf6N2E2_468 0.47 +0.9
535,096 + Pf6N2E2_468 0.59 +0.9
535,208 + Pf6N2E2_468 0.69 -1.6
535,209 - Pf6N2E2_468 0.69 -0.1
535,251 - Pf6N2E2_468 0.73 -0.7
535,251 - Pf6N2E2_468 0.73 +0.9
535,262 + Pf6N2E2_468 0.74 +0.9
535,262 + Pf6N2E2_468 0.74 -0.1
535,262 + Pf6N2E2_468 0.74 -0.3
535,262 + Pf6N2E2_468 0.74 -0.1
535,262 + Pf6N2E2_468 0.74 -1.1
535,263 - Pf6N2E2_468 0.74 +0.9
535,263 - Pf6N2E2_468 0.74 -0.1
535,263 - Pf6N2E2_468 0.74 +0.6
535,263 - Pf6N2E2_468 0.74 +0.9
535,354 + Pf6N2E2_468 0.82 +0.9
535,460 + -0.1
535,581 + +0.9
535,582 - +0.9
535,582 - +0.9
535,582 - +0.9
535,582 - +0.9
535,584 - +0.9
535,584 - -0.7
535,740 - -0.5
536,052 + Pf6N2E2_469 0.81 -0.1
536,052 + Pf6N2E2_469 0.81 -1.1
536,053 - Pf6N2E2_469 0.81 +0.9
536,110 - +0.9
536,110 - -0.4
536,591 + +0.9
536,648 + Pf6N2E2_471 0.20 +0.9
536,648 + Pf6N2E2_471 0.20 +0.8
536,648 + Pf6N2E2_471 0.20 +0.2
536,649 - Pf6N2E2_471 0.21 -0.1
536,649 - Pf6N2E2_471 0.21 +0.9
536,873 - Pf6N2E2_471 0.42 -0.1
536,942 + Pf6N2E2_471 0.48 +0.9
536,943 - Pf6N2E2_471 0.48 +0.9
537,095 + Pf6N2E2_471 0.63 +0.9
537,096 - Pf6N2E2_471 0.63 -0.1

Or see this region's nucleotide sequence