Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4473 and Pf6N2E2_4474 are separated by 81 nucleotides Pf6N2E2_4474 and Pf6N2E2_4475 are separated by 194 nucleotides Pf6N2E2_4475 and Pf6N2E2_4476 are separated by 35 nucleotides Pf6N2E2_4476 and Pf6N2E2_4477 are separated by 324 nucleotides
Pf6N2E2_4473 - HlyD family secretion protein, at 5,207,268 to 5,208,233
_4473
Pf6N2E2_4474 - FIG006231: RNA-binding protein, at 5,208,315 to 5,208,764
_4474
Pf6N2E2_4475 - FIG00953390: hypothetical protein, at 5,208,959 to 5,209,105
_4475
Pf6N2E2_4476 - 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2), at 5,209,141 to 5,209,746
_4476
Pf6N2E2_4477 - Z-ring-associated protein ZapA, at 5,210,071 to 5,210,388
_4477
Position (kb)
5208
5209
5210 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5208.215 kb on + strand at 5208.215 kb on + strand at 5208.215 kb on + strand at 5208.215 kb on + strand at 5208.215 kb on + strand at 5208.215 kb on + strand at 5208.216 kb on - strand at 5208.216 kb on - strand at 5208.252 kb on - strand at 5208.253 kb on + strand at 5208.253 kb on + strand at 5208.253 kb on + strand at 5208.254 kb on - strand at 5208.254 kb on - strand at 5208.353 kb on + strand at 5208.427 kb on + strand, within Pf6N2E2_4474 at 5208.428 kb on - strand, within Pf6N2E2_4474 at 5208.428 kb on - strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.520 kb on + strand, within Pf6N2E2_4474 at 5208.521 kb on - strand, within Pf6N2E2_4474 at 5208.521 kb on - strand, within Pf6N2E2_4474 at 5208.521 kb on - strand, within Pf6N2E2_4474 at 5208.521 kb on - strand, within Pf6N2E2_4474 at 5208.521 kb on - strand, within Pf6N2E2_4474 at 5208.521 kb on - strand, within Pf6N2E2_4474 at 5208.521 kb on - strand, within Pf6N2E2_4474 at 5208.616 kb on + strand, within Pf6N2E2_4474 at 5208.616 kb on + strand, within Pf6N2E2_4474 at 5208.617 kb on - strand, within Pf6N2E2_4474 at 5208.670 kb on + strand, within Pf6N2E2_4474 at 5208.670 kb on + strand, within Pf6N2E2_4474 at 5208.670 kb on + strand, within Pf6N2E2_4474 at 5208.670 kb on + strand, within Pf6N2E2_4474 at 5208.671 kb on - strand, within Pf6N2E2_4474 at 5208.671 kb on - strand, within Pf6N2E2_4474 at 5208.726 kb on + strand at 5208.757 kb on + strand at 5208.758 kb on - strand at 5208.771 kb on + strand at 5208.772 kb on - strand at 5208.810 kb on - strand at 5208.907 kb on + strand at 5208.937 kb on + strand at 5208.938 kb on - strand at 5208.938 kb on - strand at 5208.938 kb on - strand at 5209.153 kb on - strand at 5209.154 kb on + strand at 5209.154 kb on + strand at 5209.154 kb on + strand at 5209.154 kb on + strand at 5209.154 kb on + strand at 5209.154 kb on + strand at 5209.154 kb on + strand at 5209.154 kb on + strand at 5209.155 kb on - strand at 5209.155 kb on - strand at 5209.155 kb on - strand at 5209.155 kb on - strand at 5209.155 kb on - strand at 5209.155 kb on - strand at 5209.189 kb on + strand at 5209.189 kb on + strand at 5209.190 kb on - strand at 5209.190 kb on - strand at 5209.287 kb on - strand, within Pf6N2E2_4476 at 5209.304 kb on + strand, within Pf6N2E2_4476 at 5209.305 kb on - strand, within Pf6N2E2_4476 at 5209.514 kb on + strand, within Pf6N2E2_4476 at 5209.514 kb on + strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.515 kb on - strand, within Pf6N2E2_4476 at 5209.586 kb on + strand, within Pf6N2E2_4476 at 5209.746 kb on + strand at 5209.746 kb on + strand at 5209.746 kb on + strand at 5209.746 kb on + strand at 5209.747 kb on - strand at 5209.780 kb on + strand at 5209.781 kb on - strand at 5210.048 kb on + strand at 5210.048 kb on + strand at 5210.048 kb on + strand at 5210.049 kb on - strand at 5210.049 kb on - strand at 5210.049 kb on - strand at 5210.049 kb on - strand at 5210.051 kb on - strand at 5210.051 kb on - strand at 5210.051 kb on - strand at 5210.051 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 5,208,215 + -3.6 5,208,215 + +0.1 5,208,215 + -0.5 5,208,215 + -0.5 5,208,215 + +0.1 5,208,215 + +1.1 5,208,216 - +0.1 5,208,216 - +0.1 5,208,252 - +1.1 5,208,253 + +1.1 5,208,253 + -3.1 5,208,253 + -2.6 5,208,254 - +1.1 5,208,254 - +0.1 5,208,353 + -0.2 5,208,427 + Pf6N2E2_4474 0.25 +1.1 5,208,428 - Pf6N2E2_4474 0.25 -0.6 5,208,428 - Pf6N2E2_4474 0.25 +0.1 5,208,520 + Pf6N2E2_4474 0.46 +0.1 5,208,520 + Pf6N2E2_4474 0.46 -1.5 5,208,520 + Pf6N2E2_4474 0.46 -0.9 5,208,520 + Pf6N2E2_4474 0.46 -1.7 5,208,520 + Pf6N2E2_4474 0.46 -0.6 5,208,520 + Pf6N2E2_4474 0.46 +1.1 5,208,520 + Pf6N2E2_4474 0.46 -0.8 5,208,520 + Pf6N2E2_4474 0.46 -0.5 5,208,521 - Pf6N2E2_4474 0.46 -0.6 5,208,521 - Pf6N2E2_4474 0.46 -0.9 5,208,521 - Pf6N2E2_4474 0.46 -0.5 5,208,521 - Pf6N2E2_4474 0.46 -0.5 5,208,521 - Pf6N2E2_4474 0.46 +1.1 5,208,521 - Pf6N2E2_4474 0.46 -0.6 5,208,521 - Pf6N2E2_4474 0.46 -0.9 5,208,616 + Pf6N2E2_4474 0.67 +0.1 5,208,616 + Pf6N2E2_4474 0.67 -0.6 5,208,617 - Pf6N2E2_4474 0.67 +0.1 5,208,670 + Pf6N2E2_4474 0.79 -0.1 5,208,670 + Pf6N2E2_4474 0.79 -1.2 5,208,670 + Pf6N2E2_4474 0.79 +0.1 5,208,670 + Pf6N2E2_4474 0.79 +0.1 5,208,671 - Pf6N2E2_4474 0.79 -1.7 5,208,671 - Pf6N2E2_4474 0.79 +1.1 5,208,726 + +0.1 5,208,757 + +1.1 5,208,758 - +1.1 5,208,771 + +0.1 5,208,772 - -2.1 5,208,810 - -0.5 5,208,907 + -0.9 5,208,937 + +1.1 5,208,938 - +0.1 5,208,938 - -0.5 5,208,938 - -0.2 5,209,153 - +0.1 5,209,154 + +1.1 5,209,154 + -0.5 5,209,154 + -0.5 5,209,154 + +1.1 5,209,154 + -0.5 5,209,154 + -1.1 5,209,154 + +0.9 5,209,154 + +1.1 5,209,155 - +0.1 5,209,155 - +0.1 5,209,155 - -0.6 5,209,155 - +0.3 5,209,155 - +1.1 5,209,155 - +1.1 5,209,189 + +0.1 5,209,189 + +0.1 5,209,190 - -0.5 5,209,190 - +1.1 5,209,287 - Pf6N2E2_4476 0.24 +1.1 5,209,304 + Pf6N2E2_4476 0.27 -1.2 5,209,305 - Pf6N2E2_4476 0.27 -0.8 5,209,514 + Pf6N2E2_4476 0.62 +0.1 5,209,514 + Pf6N2E2_4476 0.62 -1.2 5,209,515 - Pf6N2E2_4476 0.62 +1.1 5,209,515 - Pf6N2E2_4476 0.62 +1.1 5,209,515 - Pf6N2E2_4476 0.62 -0.0 5,209,515 - Pf6N2E2_4476 0.62 +1.8 5,209,515 - Pf6N2E2_4476 0.62 +1.1 5,209,515 - Pf6N2E2_4476 0.62 -0.5 5,209,515 - Pf6N2E2_4476 0.62 -1.3 5,209,515 - Pf6N2E2_4476 0.62 -1.4 5,209,515 - Pf6N2E2_4476 0.62 +0.4 5,209,586 + Pf6N2E2_4476 0.73 +1.1 5,209,746 + +1.1 5,209,746 + +0.4 5,209,746 + +0.1 5,209,746 + +0.1 5,209,747 - +0.1 5,209,780 + +0.1 5,209,781 - -0.5 5,210,048 + +1.1 5,210,048 + +1.1 5,210,048 + -1.7 5,210,049 - +0.1 5,210,049 - -0.9 5,210,049 - -0.3 5,210,049 - +1.1 5,210,051 - -1.9 5,210,051 - +0.1 5,210,051 - +0.1 5,210,051 - +1.1
Or see this region's nucleotide sequence