Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_430 and Pf6N2E2_431 are separated by 3 nucleotides Pf6N2E2_431 and Pf6N2E2_432 are separated by 1 nucleotides Pf6N2E2_432 and Pf6N2E2_433 are separated by 128 nucleotides
Pf6N2E2_430 - Molybdenum transport ATP-binding protein ModC (TC 3.A.1.8.1), at 497,691 to 498,770
_430
Pf6N2E2_431 - Molybdenum transport system permease protein ModB (TC 3.A.1.8.1), at 498,774 to 499,454
_431
Pf6N2E2_432 - Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1), at 499,456 to 500,208
_432
Pf6N2E2_433 - Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), at 500,337 to 501,410
_433
Position (kb)
499
500
501 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 498.532 kb on + strand, within Pf6N2E2_430 at 498.533 kb on - strand, within Pf6N2E2_430 at 498.533 kb on - strand, within Pf6N2E2_430 at 498.609 kb on - strand, within Pf6N2E2_430 at 498.662 kb on + strand, within Pf6N2E2_430 at 498.683 kb on - strand at 498.809 kb on - strand at 498.875 kb on - strand, within Pf6N2E2_431 at 498.899 kb on - strand, within Pf6N2E2_431 at 498.899 kb on - strand, within Pf6N2E2_431 at 498.899 kb on - strand, within Pf6N2E2_431 at 499.150 kb on + strand, within Pf6N2E2_431 at 499.150 kb on + strand, within Pf6N2E2_431 at 499.151 kb on - strand, within Pf6N2E2_431 at 499.151 kb on - strand, within Pf6N2E2_431 at 499.151 kb on - strand, within Pf6N2E2_431 at 499.151 kb on - strand, within Pf6N2E2_431 at 499.151 kb on - strand, within Pf6N2E2_431 at 499.151 kb on - strand, within Pf6N2E2_431 at 499.261 kb on + strand, within Pf6N2E2_431 at 499.262 kb on - strand, within Pf6N2E2_431 at 499.262 kb on - strand, within Pf6N2E2_431 at 499.262 kb on - strand, within Pf6N2E2_431 at 499.353 kb on + strand, within Pf6N2E2_431 at 499.354 kb on - strand, within Pf6N2E2_431 at 499.432 kb on + strand at 499.433 kb on - strand at 499.457 kb on + strand at 499.511 kb on + strand at 499.511 kb on + strand at 499.512 kb on - strand at 499.640 kb on + strand, within Pf6N2E2_432 at 499.640 kb on + strand, within Pf6N2E2_432 at 499.640 kb on + strand, within Pf6N2E2_432 at 499.868 kb on + strand, within Pf6N2E2_432 at 499.868 kb on + strand, within Pf6N2E2_432 at 499.868 kb on + strand, within Pf6N2E2_432 at 499.868 kb on + strand, within Pf6N2E2_432 at 499.868 kb on + strand, within Pf6N2E2_432 at 499.869 kb on - strand, within Pf6N2E2_432 at 499.869 kb on - strand, within Pf6N2E2_432 at 499.911 kb on - strand, within Pf6N2E2_432 at 499.911 kb on - strand, within Pf6N2E2_432 at 500.021 kb on + strand, within Pf6N2E2_432 at 500.021 kb on + strand, within Pf6N2E2_432 at 500.021 kb on + strand, within Pf6N2E2_432 at 500.058 kb on + strand, within Pf6N2E2_432 at 500.058 kb on + strand, within Pf6N2E2_432 at 500.160 kb on + strand at 500.160 kb on + strand at 500.160 kb on + strand at 500.161 kb on - strand at 500.208 kb on + strand at 500.208 kb on + strand at 500.208 kb on + strand at 500.208 kb on + strand at 500.208 kb on + strand at 500.208 kb on + strand at 500.209 kb on - strand at 500.209 kb on - strand at 500.209 kb on - strand at 500.209 kb on - strand at 500.235 kb on + strand at 500.235 kb on + strand at 500.237 kb on + strand at 500.238 kb on - strand at 500.238 kb on - strand at 500.240 kb on - strand at 500.247 kb on + strand at 500.248 kb on - strand at 500.249 kb on + strand at 500.249 kb on + strand at 500.250 kb on - strand at 500.250 kb on - strand at 500.250 kb on - strand at 500.251 kb on + strand at 500.251 kb on + strand at 500.252 kb on - strand at 500.677 kb on + strand, within Pf6N2E2_433 at 500.870 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 498,532 + Pf6N2E2_430 0.78 +0.9 498,533 - Pf6N2E2_430 0.78 -0.7 498,533 - Pf6N2E2_430 0.78 -0.7 498,609 - Pf6N2E2_430 0.85 -0.8 498,662 + Pf6N2E2_430 0.90 -0.1 498,683 - -0.7 498,809 - -0.1 498,875 - Pf6N2E2_431 0.15 -2.8 498,899 - Pf6N2E2_431 0.18 +0.9 498,899 - Pf6N2E2_431 0.18 +0.9 498,899 - Pf6N2E2_431 0.18 -0.1 499,150 + Pf6N2E2_431 0.55 -0.1 499,150 + Pf6N2E2_431 0.55 +0.9 499,151 - Pf6N2E2_431 0.55 -2.3 499,151 - Pf6N2E2_431 0.55 -1.7 499,151 - Pf6N2E2_431 0.55 -0.4 499,151 - Pf6N2E2_431 0.55 -0.1 499,151 - Pf6N2E2_431 0.55 -1.1 499,151 - Pf6N2E2_431 0.55 -0.7 499,261 + Pf6N2E2_431 0.72 -3.3 499,262 - Pf6N2E2_431 0.72 -2.0 499,262 - Pf6N2E2_431 0.72 -0.3 499,262 - Pf6N2E2_431 0.72 +0.9 499,353 + Pf6N2E2_431 0.85 -1.4 499,354 - Pf6N2E2_431 0.85 +0.9 499,432 + -1.1 499,433 - -0.1 499,457 + +0.9 499,511 + -1.1 499,511 + +0.9 499,512 - -2.3 499,640 + Pf6N2E2_432 0.24 -1.3 499,640 + Pf6N2E2_432 0.24 -2.2 499,640 + Pf6N2E2_432 0.24 -2.2 499,868 + Pf6N2E2_432 0.55 -1.8 499,868 + Pf6N2E2_432 0.55 +0.9 499,868 + Pf6N2E2_432 0.55 +0.9 499,868 + Pf6N2E2_432 0.55 -1.1 499,868 + Pf6N2E2_432 0.55 -1.0 499,869 - Pf6N2E2_432 0.55 +0.9 499,869 - Pf6N2E2_432 0.55 +0.9 499,911 - Pf6N2E2_432 0.60 -3.8 499,911 - Pf6N2E2_432 0.60 -1.1 500,021 + Pf6N2E2_432 0.75 -1.8 500,021 + Pf6N2E2_432 0.75 -1.1 500,021 + Pf6N2E2_432 0.75 +0.9 500,058 + Pf6N2E2_432 0.80 -0.1 500,058 + Pf6N2E2_432 0.80 +0.1 500,160 + +0.9 500,160 + -2.8 500,160 + -2.3 500,161 - +0.9 500,208 + -2.3 500,208 + +0.9 500,208 + -1.3 500,208 + -2.0 500,208 + +0.9 500,208 + +0.9 500,209 - -0.1 500,209 - -0.1 500,209 - +0.9 500,209 - +0.9 500,235 + +0.9 500,235 + -0.7 500,237 + -0.1 500,238 - -1.2 500,238 - -0.1 500,240 - -0.1 500,247 + +0.2 500,248 - -0.7 500,249 + +0.9 500,249 + +0.9 500,250 - +0.7 500,250 - -0.1 500,250 - +0.9 500,251 + -0.7 500,251 + +0.9 500,252 - +0.9 500,677 + Pf6N2E2_433 0.32 +0.8 500,870 - Pf6N2E2_433 0.50 -0.4 501,122 - Pf6N2E2_433 0.73 -0.1 501,122 - Pf6N2E2_433 0.73 +0.9 501,122 - Pf6N2E2_433 0.73 -0.3 501,122 - Pf6N2E2_433 0.73 +0.9
Or see this region's nucleotide sequence