Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_311 and Pf6N2E2_312 are separated by 80 nucleotides Pf6N2E2_312 and Pf6N2E2_313 are separated by 164 nucleotides Pf6N2E2_313 and Pf6N2E2_314 are separated by 40 nucleotides Pf6N2E2_314 and Pf6N2E2_315 are separated by 193 nucleotides
Pf6N2E2_311 - hypothetical protein, at 378,335 to 378,685
_311
Pf6N2E2_312 - Histone acetyltransferase HPA2 and related acetyltransferases, at 378,766 to 379,197
_312
Pf6N2E2_313 - Gamma-glutamyltranspeptidase (EC 2.3.2.2), at 379,362 to 381,089
_313
Pf6N2E2_314 - Isochorismatase (EC 3.3.2.1), at 381,130 to 381,663
_314
Pf6N2E2_315 - probable transmembrane protein, at 381,857 to 382,717
_315
Position (kb) 379
380
381
382 Strain fitness (log2 ratio) -2
-1
0
1
2 at 378.422 kb on + strand, within Pf6N2E2_311 at 378.423 kb on - strand, within Pf6N2E2_311 at 378.423 kb on - strand, within Pf6N2E2_311 at 378.423 kb on - strand, within Pf6N2E2_311 at 378.423 kb on - strand, within Pf6N2E2_311 at 378.558 kb on - strand, within Pf6N2E2_311 at 378.558 kb on - strand, within Pf6N2E2_311 at 378.691 kb on + strand at 378.691 kb on + strand at 378.691 kb on + strand at 378.691 kb on + strand at 378.692 kb on - strand at 378.733 kb on + strand at 378.733 kb on + strand at 378.768 kb on - strand at 378.863 kb on + strand, within Pf6N2E2_312 at 379.007 kb on + strand, within Pf6N2E2_312 at 379.007 kb on + strand, within Pf6N2E2_312 at 379.007 kb on + strand, within Pf6N2E2_312 at 379.007 kb on + strand, within Pf6N2E2_312 at 379.008 kb on - strand, within Pf6N2E2_312 at 379.008 kb on - strand, within Pf6N2E2_312 at 379.008 kb on - strand, within Pf6N2E2_312 at 379.120 kb on - strand, within Pf6N2E2_312 at 379.120 kb on - strand, within Pf6N2E2_312 at 379.121 kb on + strand, within Pf6N2E2_312 at 379.121 kb on + strand, within Pf6N2E2_312 at 379.122 kb on - strand, within Pf6N2E2_312 at 379.189 kb on + strand at 379.210 kb on + strand at 379.290 kb on - strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.312 kb on + strand at 379.312 kb on + strand at 379.313 kb on - strand at 379.313 kb on - strand at 379.313 kb on - strand at 379.422 kb on + strand at 379.422 kb on + strand at 379.422 kb on + strand at 379.422 kb on + strand at 379.422 kb on + strand at 379.423 kb on - strand at 379.423 kb on - strand at 379.423 kb on - strand at 379.695 kb on + strand, within Pf6N2E2_313 at 379.695 kb on + strand, within Pf6N2E2_313 at 379.695 kb on + strand, within Pf6N2E2_313 at 379.695 kb on + strand, within Pf6N2E2_313 at 379.695 kb on + strand, within Pf6N2E2_313 at 379.695 kb on + strand, within Pf6N2E2_313 at 379.695 kb on + strand, within Pf6N2E2_313 at 379.695 kb on + strand, within Pf6N2E2_313 at 379.695 kb on + strand, within Pf6N2E2_313 at 379.695 kb on + strand, within Pf6N2E2_313 at 379.696 kb on - strand, within Pf6N2E2_313 at 379.696 kb on - strand, within Pf6N2E2_313 at 379.696 kb on - strand, within Pf6N2E2_313 at 379.696 kb on - strand, within Pf6N2E2_313 at 379.696 kb on - strand, within Pf6N2E2_313 at 379.696 kb on - strand, within Pf6N2E2_313 at 379.800 kb on + strand, within Pf6N2E2_313 at 379.800 kb on + strand, within Pf6N2E2_313 at 380.046 kb on + strand, within Pf6N2E2_313 at 380.046 kb on + strand, within Pf6N2E2_313 at 380.046 kb on + strand, within Pf6N2E2_313 at 380.047 kb on - strand, within Pf6N2E2_313 at 380.047 kb on - strand, within Pf6N2E2_313 at 380.047 kb on - strand, within Pf6N2E2_313 at 380.047 kb on - strand, within Pf6N2E2_313 at 380.047 kb on - strand, within Pf6N2E2_313 at 380.127 kb on + strand, within Pf6N2E2_313 at 380.127 kb on + strand, within Pf6N2E2_313 at 380.127 kb on + strand, within Pf6N2E2_313 at 380.127 kb on + strand, within Pf6N2E2_313 at 380.127 kb on + strand, within Pf6N2E2_313 at 380.128 kb on - strand, within Pf6N2E2_313 at 380.163 kb on + strand, within Pf6N2E2_313 at 380.292 kb on + strand, within Pf6N2E2_313 at 380.320 kb on - strand, within Pf6N2E2_313 at 380.337 kb on + strand, within Pf6N2E2_313 at 380.337 kb on + strand, within Pf6N2E2_313 at 380.338 kb on - strand, within Pf6N2E2_313 at 380.387 kb on + strand, within Pf6N2E2_313 at 380.497 kb on - strand, within Pf6N2E2_313 at 380.538 kb on + strand, within Pf6N2E2_313 at 380.538 kb on + strand, within Pf6N2E2_313 at 380.538 kb on + strand, within Pf6N2E2_313 at 380.538 kb on + strand, within Pf6N2E2_313 at 380.539 kb on - strand, within Pf6N2E2_313 at 380.539 kb on - strand, within Pf6N2E2_313 at 380.646 kb on + strand, within Pf6N2E2_313 at 380.647 kb on - strand, within Pf6N2E2_313 at 380.647 kb on - strand, within Pf6N2E2_313 at 380.799 kb on - strand, within Pf6N2E2_313 at 380.817 kb on + strand, within Pf6N2E2_313 at 381.039 kb on + strand at 381.039 kb on + strand at 381.040 kb on - strand at 381.040 kb on - strand at 381.040 kb on - strand at 381.040 kb on - strand at 381.040 kb on - strand at 381.304 kb on + strand, within Pf6N2E2_314 at 381.304 kb on + strand, within Pf6N2E2_314 at 381.304 kb on + strand, within Pf6N2E2_314 at 381.304 kb on + strand, within Pf6N2E2_314 at 381.304 kb on + strand, within Pf6N2E2_314 at 381.305 kb on - strand, within Pf6N2E2_314 at 381.305 kb on - strand, within Pf6N2E2_314 at 381.305 kb on - strand, within Pf6N2E2_314 at 381.305 kb on - strand, within Pf6N2E2_314 at 381.392 kb on - strand, within Pf6N2E2_314 at 381.661 kb on + strand at 381.682 kb on - strand at 381.682 kb on - strand at 381.691 kb on + strand at 381.885 kb on + strand at 381.885 kb on + strand at 381.886 kb on - strand at 381.886 kb on - strand at 382.042 kb on + strand, within Pf6N2E2_315 at 382.043 kb on - strand, within Pf6N2E2_315 at 382.043 kb on - strand, within Pf6N2E2_315 at 382.043 kb on - strand, within Pf6N2E2_315
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 378,422 + Pf6N2E2_311 0.25 -0.1 378,423 - Pf6N2E2_311 0.25 +0.9 378,423 - Pf6N2E2_311 0.25 +0.9 378,423 - Pf6N2E2_311 0.25 +0.9 378,423 - Pf6N2E2_311 0.25 -0.1 378,558 - Pf6N2E2_311 0.64 -1.1 378,558 - Pf6N2E2_311 0.64 -0.7 378,691 + -0.1 378,691 + +0.9 378,691 + +0.9 378,691 + +0.0 378,692 - -0.1 378,733 + -0.1 378,733 + +0.9 378,768 - +0.9 378,863 + Pf6N2E2_312 0.22 +0.9 379,007 + Pf6N2E2_312 0.56 +0.9 379,007 + Pf6N2E2_312 0.56 -0.7 379,007 + Pf6N2E2_312 0.56 -0.1 379,007 + Pf6N2E2_312 0.56 +0.9 379,008 - Pf6N2E2_312 0.56 +0.9 379,008 - Pf6N2E2_312 0.56 -0.7 379,008 - Pf6N2E2_312 0.56 +0.9 379,120 - Pf6N2E2_312 0.82 +0.9 379,120 - Pf6N2E2_312 0.82 -0.1 379,121 + Pf6N2E2_312 0.82 -0.3 379,121 + Pf6N2E2_312 0.82 +0.9 379,122 - Pf6N2E2_312 0.82 +0.9 379,189 + -0.1 379,210 + -1.4 379,290 - -0.1 379,308 + +0.9 379,308 + +0.9 379,308 + +0.9 379,308 + -1.2 379,308 + -0.7 379,308 + +0.9 379,308 + +0.9 379,308 + +0.9 379,308 + +0.9 379,309 - -1.1 379,309 - -0.1 379,309 - -0.7 379,309 - +1.4 379,309 - +0.9 379,309 - +0.9 379,309 - +0.9 379,309 - +0.9 379,309 - +0.9 379,309 - +0.9 379,312 + +0.9 379,312 + +0.9 379,313 - +0.9 379,313 - -0.1 379,313 - +0.9 379,422 + +0.9 379,422 + -0.7 379,422 + -0.6 379,422 + +0.9 379,422 + +0.9 379,423 - +0.9 379,423 - -0.1 379,423 - +0.9 379,695 + Pf6N2E2_313 0.19 +0.1 379,695 + Pf6N2E2_313 0.19 -0.1 379,695 + Pf6N2E2_313 0.19 -2.1 379,695 + Pf6N2E2_313 0.19 -0.1 379,695 + Pf6N2E2_313 0.19 -0.6 379,695 + Pf6N2E2_313 0.19 -0.1 379,695 + Pf6N2E2_313 0.19 -0.1 379,695 + Pf6N2E2_313 0.19 +0.9 379,695 + Pf6N2E2_313 0.19 -0.1 379,695 + Pf6N2E2_313 0.19 -0.7 379,696 - Pf6N2E2_313 0.19 +0.9 379,696 - Pf6N2E2_313 0.19 -0.7 379,696 - Pf6N2E2_313 0.19 +1.9 379,696 - Pf6N2E2_313 0.19 +0.9 379,696 - Pf6N2E2_313 0.19 -0.1 379,696 - Pf6N2E2_313 0.19 +0.9 379,800 + Pf6N2E2_313 0.25 -0.6 379,800 + Pf6N2E2_313 0.25 -0.3 380,046 + Pf6N2E2_313 0.40 +0.9 380,046 + Pf6N2E2_313 0.40 +0.9 380,046 + Pf6N2E2_313 0.40 +0.9 380,047 - Pf6N2E2_313 0.40 +0.9 380,047 - Pf6N2E2_313 0.40 +0.9 380,047 - Pf6N2E2_313 0.40 -0.1 380,047 - Pf6N2E2_313 0.40 -0.7 380,047 - Pf6N2E2_313 0.40 -0.7 380,127 + Pf6N2E2_313 0.44 -0.7 380,127 + Pf6N2E2_313 0.44 +0.9 380,127 + Pf6N2E2_313 0.44 -0.7 380,127 + Pf6N2E2_313 0.44 +0.9 380,127 + Pf6N2E2_313 0.44 +0.9 380,128 - Pf6N2E2_313 0.44 -0.2 380,163 + Pf6N2E2_313 0.46 +0.9 380,292 + Pf6N2E2_313 0.54 +0.9 380,320 - Pf6N2E2_313 0.55 +0.3 380,337 + Pf6N2E2_313 0.56 +0.3 380,337 + Pf6N2E2_313 0.56 -0.3 380,338 - Pf6N2E2_313 0.56 +0.3 380,387 + Pf6N2E2_313 0.59 +0.9 380,497 - Pf6N2E2_313 0.66 +0.5 380,538 + Pf6N2E2_313 0.68 +0.9 380,538 + Pf6N2E2_313 0.68 +0.4 380,538 + Pf6N2E2_313 0.68 -0.8 380,538 + Pf6N2E2_313 0.68 -0.2 380,539 - Pf6N2E2_313 0.68 +0.9 380,539 - Pf6N2E2_313 0.68 +0.9 380,646 + Pf6N2E2_313 0.74 +0.9 380,647 - Pf6N2E2_313 0.74 +0.3 380,647 - Pf6N2E2_313 0.74 -0.4 380,799 - Pf6N2E2_313 0.83 -1.4 380,817 + Pf6N2E2_313 0.84 +0.9 381,039 + +0.9 381,039 + +0.9 381,040 - -0.7 381,040 - -1.1 381,040 - +0.9 381,040 - -1.1 381,040 - -0.1 381,304 + Pf6N2E2_314 0.33 +0.9 381,304 + Pf6N2E2_314 0.33 -0.7 381,304 + Pf6N2E2_314 0.33 +0.9 381,304 + Pf6N2E2_314 0.33 +0.9 381,304 + Pf6N2E2_314 0.33 -0.8 381,305 - Pf6N2E2_314 0.33 -0.1 381,305 - Pf6N2E2_314 0.33 +0.9 381,305 - Pf6N2E2_314 0.33 +0.9 381,305 - Pf6N2E2_314 0.33 +0.9 381,392 - Pf6N2E2_314 0.49 -0.1 381,661 + -1.1 381,682 - +0.9 381,682 - +0.9 381,691 + -0.1 381,885 + -2.1 381,885 + +0.9 381,886 - +0.9 381,886 - -2.1 382,042 + Pf6N2E2_315 0.21 +0.9 382,043 - Pf6N2E2_315 0.22 +0.9 382,043 - Pf6N2E2_315 0.22 +0.9 382,043 - Pf6N2E2_315 0.22 +0.9
Or see this region's nucleotide sequence