Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1957 and Pf6N2E2_1958 are separated by 170 nucleotides Pf6N2E2_1958 and Pf6N2E2_1959 are separated by 188 nucleotides Pf6N2E2_1959 and Pf6N2E2_1960 are separated by 57 nucleotides
Pf6N2E2_1957 - putative inner membrane protein, at 2,321,749 to 2,323,065
_1957
Pf6N2E2_1958 - Predicted dinucleotide-binding enzymes, at 2,323,236 to 2,323,778
_1958
Pf6N2E2_1959 - D-sorbitol dehydrogenase (EC 1.1.1.14) (from data) , at 2,323,967 to 2,324,740
_1959
Pf6N2E2_1960 - ABC transporter for D-sorbitol, ATPase component (from data) , at 2,324,798 to 2,325,895
_1960
Position (kb)
2323
2324
2325 Strain fitness (log2 ratio)
-1
0
1 at 2322.967 kb on - strand at 2322.967 kb on - strand at 2322.967 kb on - strand at 2323.029 kb on + strand at 2323.071 kb on - strand at 2323.201 kb on + strand at 2323.201 kb on + strand at 2323.202 kb on - strand at 2323.202 kb on - strand at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.462 kb on - strand, within Pf6N2E2_1958 at 2323.507 kb on - strand, within Pf6N2E2_1958 at 2323.777 kb on - strand at 2323.777 kb on - strand at 2323.800 kb on + strand at 2323.804 kb on + strand at 2323.805 kb on - strand at 2323.805 kb on - strand at 2323.805 kb on - strand at 2323.824 kb on + strand at 2323.999 kb on - strand at 2324.016 kb on + strand at 2324.123 kb on + strand, within Pf6N2E2_1959 at 2324.251 kb on + strand, within Pf6N2E2_1959 at 2324.578 kb on - strand, within Pf6N2E2_1959 at 2324.637 kb on - strand, within Pf6N2E2_1959 at 2324.664 kb on + strand at 2324.664 kb on + strand at 2324.664 kb on + strand at 2324.665 kb on - strand at 2324.665 kb on - strand at 2324.665 kb on - strand at 2324.665 kb on - strand at 2324.722 kb on + strand at 2324.748 kb on - strand at 2324.881 kb on - strand at 2324.881 kb on - strand at 2325.232 kb on - strand, within Pf6N2E2_1960 at 2325.232 kb on - strand, within Pf6N2E2_1960 at 2325.330 kb on + strand, within Pf6N2E2_1960 at 2325.331 kb on - strand, within Pf6N2E2_1960 at 2325.331 kb on - strand, within Pf6N2E2_1960 at 2325.355 kb on + strand, within Pf6N2E2_1960 at 2325.387 kb on - strand, within Pf6N2E2_1960 at 2325.502 kb on + strand, within Pf6N2E2_1960 at 2325.502 kb on + strand, within Pf6N2E2_1960 at 2325.503 kb on - strand, within Pf6N2E2_1960 at 2325.645 kb on + strand, within Pf6N2E2_1960 at 2325.645 kb on + strand, within Pf6N2E2_1960 at 2325.645 kb on + strand, within Pf6N2E2_1960 at 2325.646 kb on - strand, within Pf6N2E2_1960 at 2325.646 kb on - strand, within Pf6N2E2_1960 at 2325.646 kb on - strand, within Pf6N2E2_1960
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 2,322,967 - -0.5 2,322,967 - +0.9 2,322,967 - +0.9 2,323,029 + +0.9 2,323,071 - +0.9 2,323,201 + +0.9 2,323,201 + -1.7 2,323,202 - -0.2 2,323,202 - +0.9 2,323,459 + Pf6N2E2_1958 0.41 +0.5 2,323,459 + Pf6N2E2_1958 0.41 -0.6 2,323,459 + Pf6N2E2_1958 0.41 -0.7 2,323,459 + Pf6N2E2_1958 0.41 -1.1 2,323,462 - Pf6N2E2_1958 0.42 -0.1 2,323,507 - Pf6N2E2_1958 0.50 -0.1 2,323,777 - -0.9 2,323,777 - -1.1 2,323,800 + +0.9 2,323,804 + -0.1 2,323,805 - +0.9 2,323,805 - +0.9 2,323,805 - +0.9 2,323,824 + -0.4 2,323,999 - -0.1 2,324,016 + +0.7 2,324,123 + Pf6N2E2_1959 0.20 -1.4 2,324,251 + Pf6N2E2_1959 0.37 +0.9 2,324,578 - Pf6N2E2_1959 0.79 -0.7 2,324,637 - Pf6N2E2_1959 0.87 +0.9 2,324,664 + -0.1 2,324,664 + -0.7 2,324,664 + +0.9 2,324,665 - -0.7 2,324,665 - -0.1 2,324,665 - +0.9 2,324,665 - -0.7 2,324,722 + -0.6 2,324,748 - -0.1 2,324,881 - +0.9 2,324,881 - +0.9 2,325,232 - Pf6N2E2_1960 0.40 -0.1 2,325,232 - Pf6N2E2_1960 0.40 +0.9 2,325,330 + Pf6N2E2_1960 0.48 +0.9 2,325,331 - Pf6N2E2_1960 0.49 -1.1 2,325,331 - Pf6N2E2_1960 0.49 +0.9 2,325,355 + Pf6N2E2_1960 0.51 +0.9 2,325,387 - Pf6N2E2_1960 0.54 +0.9 2,325,502 + Pf6N2E2_1960 0.64 -0.1 2,325,502 + Pf6N2E2_1960 0.64 +0.9 2,325,503 - Pf6N2E2_1960 0.64 +0.9 2,325,645 + Pf6N2E2_1960 0.77 -0.1 2,325,645 + Pf6N2E2_1960 0.77 +0.9 2,325,645 + Pf6N2E2_1960 0.77 +0.9 2,325,646 - Pf6N2E2_1960 0.77 +0.6 2,325,646 - Pf6N2E2_1960 0.77 -0.3 2,325,646 - Pf6N2E2_1960 0.77 +0.9
Or see this region's nucleotide sequence