Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1664 and Pf6N2E2_1665 overlap by 6 nucleotides Pf6N2E2_1665 and Pf6N2E2_1666 are separated by 210 nucleotides
Pf6N2E2_1664 - Nitrate/nitrite transporter, at 1,970,587 to 1,971,969
_1664
Pf6N2E2_1665 - hypothetical protein, at 1,971,964 to 1,972,113
_1665
Pf6N2E2_1666 - Transcriptional regulator, LysR family, at 1,972,324 to 1,973,262
_1666
Position (kb) 1971
1972
1973 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1971.137 kb on + strand, within Pf6N2E2_1664 at 1971.427 kb on + strand, within Pf6N2E2_1664 at 1971.509 kb on + strand, within Pf6N2E2_1664 at 1971.510 kb on - strand, within Pf6N2E2_1664 at 1971.518 kb on + strand, within Pf6N2E2_1664 at 1971.519 kb on - strand, within Pf6N2E2_1664 at 1971.519 kb on - strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.579 kb on - strand, within Pf6N2E2_1664 at 1971.579 kb on - strand, within Pf6N2E2_1664 at 1971.579 kb on - strand, within Pf6N2E2_1664 at 1971.630 kb on - strand, within Pf6N2E2_1664 at 1971.770 kb on + strand, within Pf6N2E2_1664 at 1971.770 kb on + strand, within Pf6N2E2_1664 at 1971.770 kb on + strand, within Pf6N2E2_1664 at 1971.770 kb on + strand, within Pf6N2E2_1664 at 1971.771 kb on - strand, within Pf6N2E2_1664 at 1971.854 kb on + strand at 1971.854 kb on + strand at 1971.855 kb on - strand at 1971.899 kb on + strand at 1971.900 kb on - strand at 1971.974 kb on + strand at 1971.975 kb on - strand at 1971.975 kb on - strand at 1971.975 kb on - strand at 1972.041 kb on + strand, within Pf6N2E2_1665 at 1972.041 kb on + strand, within Pf6N2E2_1665 at 1972.041 kb on + strand, within Pf6N2E2_1665 at 1972.041 kb on + strand, within Pf6N2E2_1665 at 1972.042 kb on - strand, within Pf6N2E2_1665 at 1972.131 kb on + strand at 1972.131 kb on + strand at 1972.131 kb on + strand at 1972.131 kb on + strand at 1972.206 kb on + strand at 1972.239 kb on + strand at 1972.709 kb on + strand, within Pf6N2E2_1666 at 1972.710 kb on - strand, within Pf6N2E2_1666 at 1972.710 kb on - strand, within Pf6N2E2_1666 at 1972.839 kb on + strand, within Pf6N2E2_1666 at 1973.028 kb on - strand, within Pf6N2E2_1666 at 1973.035 kb on + strand, within Pf6N2E2_1666
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 1,971,137 + Pf6N2E2_1664 0.40 -0.1 1,971,427 + Pf6N2E2_1664 0.61 -0.7 1,971,509 + Pf6N2E2_1664 0.67 +0.9 1,971,510 - Pf6N2E2_1664 0.67 +0.9 1,971,518 + Pf6N2E2_1664 0.67 -0.7 1,971,519 - Pf6N2E2_1664 0.67 +0.9 1,971,519 - Pf6N2E2_1664 0.67 +0.1 1,971,578 + Pf6N2E2_1664 0.72 +0.9 1,971,578 + Pf6N2E2_1664 0.72 -1.7 1,971,578 + Pf6N2E2_1664 0.72 +0.9 1,971,578 + Pf6N2E2_1664 0.72 -1.7 1,971,579 - Pf6N2E2_1664 0.72 +0.9 1,971,579 - Pf6N2E2_1664 0.72 -1.1 1,971,579 - Pf6N2E2_1664 0.72 -0.7 1,971,630 - Pf6N2E2_1664 0.75 +0.9 1,971,770 + Pf6N2E2_1664 0.86 -0.1 1,971,770 + Pf6N2E2_1664 0.86 -0.4 1,971,770 + Pf6N2E2_1664 0.86 +0.9 1,971,770 + Pf6N2E2_1664 0.86 -2.0 1,971,771 - Pf6N2E2_1664 0.86 -0.1 1,971,854 + -1.5 1,971,854 + -0.7 1,971,855 - -0.7 1,971,899 + -1.1 1,971,900 - -0.4 1,971,974 + -0.5 1,971,975 - -0.3 1,971,975 - -0.7 1,971,975 - +0.9 1,972,041 + Pf6N2E2_1665 0.51 +0.9 1,972,041 + Pf6N2E2_1665 0.51 -0.1 1,972,041 + Pf6N2E2_1665 0.51 -1.1 1,972,041 + Pf6N2E2_1665 0.51 -1.5 1,972,042 - Pf6N2E2_1665 0.52 +0.9 1,972,131 + +0.9 1,972,131 + -0.5 1,972,131 + -1.1 1,972,131 + -0.1 1,972,206 + -0.8 1,972,239 + +0.9 1,972,709 + Pf6N2E2_1666 0.41 +0.9 1,972,710 - Pf6N2E2_1666 0.41 +0.3 1,972,710 - Pf6N2E2_1666 0.41 +0.9 1,972,839 + Pf6N2E2_1666 0.55 +0.9 1,973,028 - Pf6N2E2_1666 0.75 -2.8 1,973,035 + Pf6N2E2_1666 0.76 +0.4
Or see this region's nucleotide sequence