Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_163

Experiment: Ying_Nucleobase14 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_162 and Pf6N2E2_163 overlap by 4 nucleotidesPf6N2E2_163 and Pf6N2E2_164 are separated by 3 nucleotides Pf6N2E2_162 - Ribose ABC transport system, ATP-binding protein RbsA (TC 3.A.1.2.1), at 215,296 to 216,849 _162 Pf6N2E2_163 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 216,846 to 217,823 _163 Pf6N2E2_164 - Ribose operon repressor, at 217,827 to 218,846 _164 Position (kb) 216 217 218Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 215.893 kb on + strand, within Pf6N2E2_162at 215.893 kb on + strand, within Pf6N2E2_162at 215.893 kb on + strand, within Pf6N2E2_162at 215.893 kb on + strand, within Pf6N2E2_162at 215.893 kb on + strand, within Pf6N2E2_162at 215.893 kb on + strand, within Pf6N2E2_162at 215.894 kb on - strand, within Pf6N2E2_162at 215.894 kb on - strand, within Pf6N2E2_162at 215.894 kb on - strand, within Pf6N2E2_162at 215.894 kb on - strand, within Pf6N2E2_162at 215.894 kb on - strand, within Pf6N2E2_162at 215.989 kb on + strand, within Pf6N2E2_162at 215.989 kb on + strand, within Pf6N2E2_162at 215.989 kb on + strand, within Pf6N2E2_162at 215.989 kb on + strand, within Pf6N2E2_162at 215.990 kb on - strand, within Pf6N2E2_162at 215.990 kb on - strand, within Pf6N2E2_162at 215.990 kb on - strand, within Pf6N2E2_162at 216.087 kb on + strand, within Pf6N2E2_162at 216.156 kb on + strand, within Pf6N2E2_162at 216.616 kb on + strand, within Pf6N2E2_162at 216.616 kb on + strand, within Pf6N2E2_162at 216.616 kb on + strand, within Pf6N2E2_162at 216.616 kb on + strand, within Pf6N2E2_162at 216.617 kb on - strand, within Pf6N2E2_162at 216.617 kb on - strand, within Pf6N2E2_162at 216.617 kb on - strand, within Pf6N2E2_162at 216.617 kb on - strand, within Pf6N2E2_162at 216.888 kb on + strandat 216.889 kb on - strandat 216.903 kb on + strandat 216.904 kb on - strandat 216.975 kb on + strand, within Pf6N2E2_163at 216.975 kb on + strand, within Pf6N2E2_163at 216.990 kb on - strand, within Pf6N2E2_163at 217.302 kb on + strand, within Pf6N2E2_163at 217.302 kb on + strand, within Pf6N2E2_163at 217.303 kb on - strand, within Pf6N2E2_163at 217.303 kb on - strand, within Pf6N2E2_163at 217.492 kb on - strand, within Pf6N2E2_163at 217.505 kb on - strand, within Pf6N2E2_163at 217.631 kb on + strand, within Pf6N2E2_163at 217.631 kb on + strand, within Pf6N2E2_163at 217.791 kb on + strandat 217.792 kb on - strandat 217.847 kb on - strandat 217.959 kb on + strand, within Pf6N2E2_164at 217.959 kb on + strand, within Pf6N2E2_164at 217.960 kb on - strand, within Pf6N2E2_164at 218.043 kb on + strand, within Pf6N2E2_164at 218.044 kb on - strand, within Pf6N2E2_164at 218.044 kb on - strand, within Pf6N2E2_164at 218.076 kb on + strand, within Pf6N2E2_164at 218.076 kb on + strand, within Pf6N2E2_164at 218.076 kb on + strand, within Pf6N2E2_164at 218.145 kb on + strand, within Pf6N2E2_164at 218.145 kb on + strand, within Pf6N2E2_164at 218.291 kb on - strand, within Pf6N2E2_164at 218.310 kb on + strand, within Pf6N2E2_164at 218.310 kb on + strand, within Pf6N2E2_164at 218.311 kb on - strand, within Pf6N2E2_164at 218.319 kb on + strand, within Pf6N2E2_164at 218.320 kb on - strand, within Pf6N2E2_164at 218.387 kb on + strand, within Pf6N2E2_164at 218.524 kb on + strand, within Pf6N2E2_164at 218.524 kb on + strand, within Pf6N2E2_164at 218.525 kb on - strand, within Pf6N2E2_164at 218.525 kb on - strand, within Pf6N2E2_164at 218.662 kb on - strand, within Pf6N2E2_164at 218.662 kb on - strand, within Pf6N2E2_164at 218.789 kb on + strandat 218.789 kb on + strandat 218.789 kb on + strandat 218.789 kb on + strandat 218.789 kb on + strandat 218.789 kb on + strandat 218.789 kb on + strandat 218.789 kb on + strandat 218.789 kb on + strandat 218.789 kb on + strandat 218.790 kb on - strandat 218.790 kb on - strandat 218.790 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2
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215,893 + Pf6N2E2_162 0.38 +0.9
215,893 + Pf6N2E2_162 0.38 +0.9
215,893 + Pf6N2E2_162 0.38 -1.8
215,893 + Pf6N2E2_162 0.38 +0.9
215,893 + Pf6N2E2_162 0.38 -1.6
215,893 + Pf6N2E2_162 0.38 -0.6
215,894 - Pf6N2E2_162 0.38 -0.1
215,894 - Pf6N2E2_162 0.38 -0.1
215,894 - Pf6N2E2_162 0.38 +0.9
215,894 - Pf6N2E2_162 0.38 -0.4
215,894 - Pf6N2E2_162 0.38 +0.9
215,989 + Pf6N2E2_162 0.45 +0.9
215,989 + Pf6N2E2_162 0.45 -3.9
215,989 + Pf6N2E2_162 0.45 +0.9
215,989 + Pf6N2E2_162 0.45 -2.5
215,990 - Pf6N2E2_162 0.45 -1.5
215,990 - Pf6N2E2_162 0.45 -0.6
215,990 - Pf6N2E2_162 0.45 -0.1
216,087 + Pf6N2E2_162 0.51 +0.9
216,156 + Pf6N2E2_162 0.55 -3.0
216,616 + Pf6N2E2_162 0.85 +0.9
216,616 + Pf6N2E2_162 0.85 +0.9
216,616 + Pf6N2E2_162 0.85 -2.4
216,616 + Pf6N2E2_162 0.85 -1.5
216,617 - Pf6N2E2_162 0.85 +0.9
216,617 - Pf6N2E2_162 0.85 +0.9
216,617 - Pf6N2E2_162 0.85 -2.6
216,617 - Pf6N2E2_162 0.85 +0.9
216,888 + -0.6
216,889 - -0.1
216,903 + +0.9
216,904 - -0.1
216,975 + Pf6N2E2_163 0.13 +0.9
216,975 + Pf6N2E2_163 0.13 +0.9
216,990 - Pf6N2E2_163 0.15 -0.1
217,302 + Pf6N2E2_163 0.47 +0.9
217,302 + Pf6N2E2_163 0.47 +0.9
217,303 - Pf6N2E2_163 0.47 +0.9
217,303 - Pf6N2E2_163 0.47 -0.1
217,492 - Pf6N2E2_163 0.66 -3.4
217,505 - Pf6N2E2_163 0.67 -1.9
217,631 + Pf6N2E2_163 0.80 +0.9
217,631 + Pf6N2E2_163 0.80 -2.1
217,791 + +0.9
217,792 - +0.9
217,847 - -0.6
217,959 + Pf6N2E2_164 0.13 +0.9
217,959 + Pf6N2E2_164 0.13 -1.5
217,960 - Pf6N2E2_164 0.13 -0.6
218,043 + Pf6N2E2_164 0.21 +0.9
218,044 - Pf6N2E2_164 0.21 +0.9
218,044 - Pf6N2E2_164 0.21 +0.9
218,076 + Pf6N2E2_164 0.24 +0.9
218,076 + Pf6N2E2_164 0.24 -1.5
218,076 + Pf6N2E2_164 0.24 +0.9
218,145 + Pf6N2E2_164 0.31 +0.9
218,145 + Pf6N2E2_164 0.31 -0.1
218,291 - Pf6N2E2_164 0.45 -4.1
218,310 + Pf6N2E2_164 0.47 -1.4
218,310 + Pf6N2E2_164 0.47 +0.9
218,311 - Pf6N2E2_164 0.47 -0.1
218,319 + Pf6N2E2_164 0.48 +0.9
218,320 - Pf6N2E2_164 0.48 -0.6
218,387 + Pf6N2E2_164 0.55 -1.7
218,524 + Pf6N2E2_164 0.68 -0.6
218,524 + Pf6N2E2_164 0.68 +0.9
218,525 - Pf6N2E2_164 0.68 +0.9
218,525 - Pf6N2E2_164 0.68 +0.9
218,662 - Pf6N2E2_164 0.82 -1.9
218,662 - Pf6N2E2_164 0.82 -3.1
218,789 + -0.1
218,789 + -0.1
218,789 + -0.1
218,789 + +0.9
218,789 + -0.6
218,789 + +1.1
218,789 + +0.9
218,789 + +1.3
218,789 + -0.1
218,789 + -0.1
218,790 - +0.9
218,790 - -2.9
218,790 - -2.6

Or see this region's nucleotide sequence