Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5518

Experiment: Ying_Nucleobase14 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5516 and Pf6N2E2_5517 are separated by 93 nucleotidesPf6N2E2_5517 and Pf6N2E2_5518 are separated by 11 nucleotidesPf6N2E2_5518 and Pf6N2E2_5519 are separated by 135 nucleotidesPf6N2E2_5519 and Pf6N2E2_5520 are separated by 2 nucleotides Pf6N2E2_5516 - Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1), at 6,335,232 to 6,336,449 _5516 Pf6N2E2_5517 - Glutathione S-transferase (EC 2.5.1.18), at 6,336,543 to 6,337,175 _5517 Pf6N2E2_5518 - Mutator mutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (EC 3.6.1.-) / Thiamin-phosphate pyrophosphorylase-like protein, at 6,337,187 to 6,338,116 _5518 Pf6N2E2_5519 - ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17), at 6,338,252 to 6,338,833 _5519 Pf6N2E2_5520 - Sensory box histidine kinase, at 6,338,836 to 6,340,872 _5520 Position (kb) 6337 6338 6339Strain fitness (log2 ratio) -2 -1 0 1 2at 6336.304 kb on - strand, within Pf6N2E2_5516at 6336.447 kb on + strandat 6336.447 kb on + strandat 6336.448 kb on - strandat 6336.525 kb on + strandat 6336.526 kb on - strandat 6336.526 kb on - strandat 6336.526 kb on - strandat 6336.630 kb on + strand, within Pf6N2E2_5517at 6336.631 kb on - strand, within Pf6N2E2_5517at 6336.631 kb on - strand, within Pf6N2E2_5517at 6336.631 kb on - strand, within Pf6N2E2_5517at 6336.688 kb on - strand, within Pf6N2E2_5517at 6336.688 kb on - strand, within Pf6N2E2_5517at 6336.762 kb on + strand, within Pf6N2E2_5517at 6337.002 kb on + strand, within Pf6N2E2_5517at 6337.002 kb on + strand, within Pf6N2E2_5517at 6337.003 kb on - strand, within Pf6N2E2_5517at 6337.068 kb on + strand, within Pf6N2E2_5517at 6337.068 kb on + strand, within Pf6N2E2_5517at 6337.068 kb on + strand, within Pf6N2E2_5517at 6337.068 kb on + strand, within Pf6N2E2_5517at 6337.069 kb on - strand, within Pf6N2E2_5517at 6337.069 kb on - strand, within Pf6N2E2_5517at 6337.069 kb on - strand, within Pf6N2E2_5517at 6337.069 kb on - strand, within Pf6N2E2_5517at 6337.069 kb on - strand, within Pf6N2E2_5517at 6337.101 kb on + strand, within Pf6N2E2_5517at 6337.101 kb on + strand, within Pf6N2E2_5517at 6337.102 kb on - strand, within Pf6N2E2_5517at 6337.116 kb on + strandat 6337.394 kb on + strand, within Pf6N2E2_5518at 6337.508 kb on + strand, within Pf6N2E2_5518at 6337.508 kb on + strand, within Pf6N2E2_5518at 6337.509 kb on - strand, within Pf6N2E2_5518at 6337.509 kb on - strand, within Pf6N2E2_5518at 6337.509 kb on - strand, within Pf6N2E2_5518at 6337.509 kb on - strand, within Pf6N2E2_5518at 6337.509 kb on - strand, within Pf6N2E2_5518at 6337.509 kb on - strand, within Pf6N2E2_5518at 6337.509 kb on - strand, within Pf6N2E2_5518at 6337.509 kb on - strand, within Pf6N2E2_5518at 6337.565 kb on + strand, within Pf6N2E2_5518at 6337.566 kb on - strand, within Pf6N2E2_5518at 6337.653 kb on + strand, within Pf6N2E2_5518at 6337.654 kb on - strand, within Pf6N2E2_5518at 6337.654 kb on - strand, within Pf6N2E2_5518at 6337.683 kb on - strand, within Pf6N2E2_5518at 6337.870 kb on + strand, within Pf6N2E2_5518at 6337.959 kb on + strand, within Pf6N2E2_5518at 6337.959 kb on + strand, within Pf6N2E2_5518at 6337.960 kb on - strand, within Pf6N2E2_5518at 6337.960 kb on - strand, within Pf6N2E2_5518at 6338.009 kb on - strand, within Pf6N2E2_5518at 6338.018 kb on + strand, within Pf6N2E2_5518at 6338.018 kb on + strand, within Pf6N2E2_5518at 6338.018 kb on + strand, within Pf6N2E2_5518at 6338.018 kb on + strand, within Pf6N2E2_5518at 6338.018 kb on + strand, within Pf6N2E2_5518at 6338.019 kb on - strand, within Pf6N2E2_5518at 6338.356 kb on + strand, within Pf6N2E2_5519at 6338.356 kb on + strand, within Pf6N2E2_5519at 6338.357 kb on - strand, within Pf6N2E2_5519at 6338.358 kb on + strand, within Pf6N2E2_5519at 6338.358 kb on + strand, within Pf6N2E2_5519at 6338.358 kb on + strand, within Pf6N2E2_5519at 6338.359 kb on - strand, within Pf6N2E2_5519at 6338.457 kb on - strand, within Pf6N2E2_5519at 6338.569 kb on - strand, within Pf6N2E2_5519at 6338.690 kb on + strand, within Pf6N2E2_5519at 6338.690 kb on + strand, within Pf6N2E2_5519at 6338.691 kb on - strand, within Pf6N2E2_5519at 6338.714 kb on - strand, within Pf6N2E2_5519at 6338.808 kb on + strandat 6338.972 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2
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6,336,304 - Pf6N2E2_5516 0.88 +1.0
6,336,447 + -0.6
6,336,447 + -0.6
6,336,448 - -0.0
6,336,525 + +1.0
6,336,526 - +1.0
6,336,526 - -0.6
6,336,526 - -0.6
6,336,630 + Pf6N2E2_5517 0.14 +1.0
6,336,631 - Pf6N2E2_5517 0.14 -0.6
6,336,631 - Pf6N2E2_5517 0.14 +1.0
6,336,631 - Pf6N2E2_5517 0.14 -0.0
6,336,688 - Pf6N2E2_5517 0.23 -0.0
6,336,688 - Pf6N2E2_5517 0.23 -2.0
6,336,762 + Pf6N2E2_5517 0.35 +1.0
6,337,002 + Pf6N2E2_5517 0.73 -0.6
6,337,002 + Pf6N2E2_5517 0.73 +1.0
6,337,003 - Pf6N2E2_5517 0.73 +1.0
6,337,068 + Pf6N2E2_5517 0.83 -0.0
6,337,068 + Pf6N2E2_5517 0.83 +1.0
6,337,068 + Pf6N2E2_5517 0.83 -0.0
6,337,068 + Pf6N2E2_5517 0.83 -0.0
6,337,069 - Pf6N2E2_5517 0.83 +1.0
6,337,069 - Pf6N2E2_5517 0.83 -0.0
6,337,069 - Pf6N2E2_5517 0.83 +1.0
6,337,069 - Pf6N2E2_5517 0.83 +0.4
6,337,069 - Pf6N2E2_5517 0.83 -0.0
6,337,101 + Pf6N2E2_5517 0.88 -1.0
6,337,101 + Pf6N2E2_5517 0.88 -0.0
6,337,102 - Pf6N2E2_5517 0.88 -0.6
6,337,116 + +0.7
6,337,394 + Pf6N2E2_5518 0.22 -1.1
6,337,508 + Pf6N2E2_5518 0.35 -1.0
6,337,508 + Pf6N2E2_5518 0.35 -0.0
6,337,509 - Pf6N2E2_5518 0.35 -0.6
6,337,509 - Pf6N2E2_5518 0.35 +1.0
6,337,509 - Pf6N2E2_5518 0.35 -0.0
6,337,509 - Pf6N2E2_5518 0.35 -2.5
6,337,509 - Pf6N2E2_5518 0.35 +1.0
6,337,509 - Pf6N2E2_5518 0.35 -2.0
6,337,509 - Pf6N2E2_5518 0.35 -0.0
6,337,509 - Pf6N2E2_5518 0.35 -1.2
6,337,565 + Pf6N2E2_5518 0.41 +1.0
6,337,566 - Pf6N2E2_5518 0.41 -0.0
6,337,653 + Pf6N2E2_5518 0.50 -2.5
6,337,654 - Pf6N2E2_5518 0.50 -0.0
6,337,654 - Pf6N2E2_5518 0.50 +1.0
6,337,683 - Pf6N2E2_5518 0.53 +1.0
6,337,870 + Pf6N2E2_5518 0.73 +1.0
6,337,959 + Pf6N2E2_5518 0.83 +1.0
6,337,959 + Pf6N2E2_5518 0.83 -0.6
6,337,960 - Pf6N2E2_5518 0.83 -0.0
6,337,960 - Pf6N2E2_5518 0.83 +1.0
6,338,009 - Pf6N2E2_5518 0.88 +1.0
6,338,018 + Pf6N2E2_5518 0.89 +1.0
6,338,018 + Pf6N2E2_5518 0.89 +1.0
6,338,018 + Pf6N2E2_5518 0.89 -2.3
6,338,018 + Pf6N2E2_5518 0.89 -1.3
6,338,018 + Pf6N2E2_5518 0.89 -2.4
6,338,019 - Pf6N2E2_5518 0.89 +1.0
6,338,356 + Pf6N2E2_5519 0.18 -1.0
6,338,356 + Pf6N2E2_5519 0.18 +1.0
6,338,357 - Pf6N2E2_5519 0.18 -0.9
6,338,358 + Pf6N2E2_5519 0.18 +1.0
6,338,358 + Pf6N2E2_5519 0.18 +1.0
6,338,358 + Pf6N2E2_5519 0.18 +0.4
6,338,359 - Pf6N2E2_5519 0.18 -0.6
6,338,457 - Pf6N2E2_5519 0.35 -0.4
6,338,569 - Pf6N2E2_5519 0.54 +0.6
6,338,690 + Pf6N2E2_5519 0.75 +1.0
6,338,690 + Pf6N2E2_5519 0.75 -0.6
6,338,691 - Pf6N2E2_5519 0.75 -0.0
6,338,714 - Pf6N2E2_5519 0.79 +1.0
6,338,808 + +2.0
6,338,972 + -0.0

Or see this region's nucleotide sequence