Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5281 and Pf6N2E2_5282 are separated by 261 nucleotides Pf6N2E2_5282 and Pf6N2E2_5283 are separated by 42 nucleotides Pf6N2E2_5283 and Pf6N2E2_5284 are separated by 34 nucleotides Pf6N2E2_5284 and Pf6N2E2_5285 are separated by 268 nucleotides
Pf6N2E2_5281 - Alpha/beta hydrolase fold (EC 3.8.1.5), at 6,072,293 to 6,073,195
_5281
Pf6N2E2_5282 - Oxidoreductase, short chain dehydrogenase/reductase family, at 6,073,457 to 6,074,368
_5282
Pf6N2E2_5283 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 6,074,411 to 6,074,704
_5283
Pf6N2E2_5284 - hypothetical protein, at 6,074,739 to 6,074,870
_5284
Pf6N2E2_5285 - FIG067310: hypothetical protein, at 6,075,139 to 6,076,917
_5285
Position (kb)
6073
6074
6075 Strain fitness (log2 ratio)
-2
-1
0
1 at 6072.533 kb on + strand, within Pf6N2E2_5281 at 6072.533 kb on + strand, within Pf6N2E2_5281 at 6072.534 kb on - strand, within Pf6N2E2_5281 at 6072.620 kb on + strand, within Pf6N2E2_5281 at 6072.620 kb on + strand, within Pf6N2E2_5281 at 6072.628 kb on + strand, within Pf6N2E2_5281 at 6072.660 kb on - strand, within Pf6N2E2_5281 at 6072.660 kb on - strand, within Pf6N2E2_5281 at 6072.845 kb on - strand, within Pf6N2E2_5281 at 6072.858 kb on + strand, within Pf6N2E2_5281 at 6072.858 kb on + strand, within Pf6N2E2_5281 at 6072.858 kb on + strand, within Pf6N2E2_5281 at 6072.858 kb on + strand, within Pf6N2E2_5281 at 6072.859 kb on - strand, within Pf6N2E2_5281 at 6072.859 kb on - strand, within Pf6N2E2_5281 at 6072.923 kb on + strand, within Pf6N2E2_5281 at 6072.923 kb on + strand, within Pf6N2E2_5281 at 6072.923 kb on + strand, within Pf6N2E2_5281 at 6072.923 kb on + strand, within Pf6N2E2_5281 at 6072.923 kb on + strand, within Pf6N2E2_5281 at 6072.923 kb on + strand, within Pf6N2E2_5281 at 6072.923 kb on + strand, within Pf6N2E2_5281 at 6072.923 kb on + strand, within Pf6N2E2_5281 at 6072.924 kb on - strand, within Pf6N2E2_5281 at 6072.924 kb on - strand, within Pf6N2E2_5281 at 6072.924 kb on - strand, within Pf6N2E2_5281 at 6072.924 kb on - strand, within Pf6N2E2_5281 at 6072.924 kb on - strand, within Pf6N2E2_5281 at 6072.924 kb on - strand, within Pf6N2E2_5281 at 6072.924 kb on - strand, within Pf6N2E2_5281 at 6072.924 kb on - strand, within Pf6N2E2_5281 at 6072.924 kb on - strand, within Pf6N2E2_5281 at 6072.924 kb on - strand, within Pf6N2E2_5281 at 6072.926 kb on - strand, within Pf6N2E2_5281 at 6073.139 kb on + strand at 6073.139 kb on + strand at 6073.140 kb on - strand at 6073.140 kb on - strand at 6073.140 kb on - strand at 6073.199 kb on + strand at 6073.200 kb on - strand at 6073.250 kb on - strand at 6073.250 kb on - strand at 6073.250 kb on - strand at 6073.250 kb on - strand at 6073.250 kb on - strand at 6073.250 kb on - strand at 6073.704 kb on + strand, within Pf6N2E2_5282 at 6073.705 kb on - strand, within Pf6N2E2_5282 at 6073.815 kb on - strand, within Pf6N2E2_5282 at 6073.839 kb on - strand, within Pf6N2E2_5282 at 6073.839 kb on - strand, within Pf6N2E2_5282 at 6073.839 kb on - strand, within Pf6N2E2_5282 at 6073.839 kb on - strand, within Pf6N2E2_5282 at 6073.839 kb on - strand, within Pf6N2E2_5282 at 6073.839 kb on - strand, within Pf6N2E2_5282 at 6073.839 kb on - strand, within Pf6N2E2_5282 at 6073.922 kb on + strand, within Pf6N2E2_5282 at 6073.923 kb on - strand, within Pf6N2E2_5282 at 6073.923 kb on - strand, within Pf6N2E2_5282 at 6073.923 kb on - strand, within Pf6N2E2_5282 at 6073.923 kb on - strand, within Pf6N2E2_5282 at 6073.947 kb on - strand, within Pf6N2E2_5282 at 6073.970 kb on + strand, within Pf6N2E2_5282 at 6073.971 kb on - strand, within Pf6N2E2_5282 at 6073.971 kb on - strand, within Pf6N2E2_5282 at 6074.014 kb on + strand, within Pf6N2E2_5282 at 6074.014 kb on + strand, within Pf6N2E2_5282 at 6074.015 kb on - strand, within Pf6N2E2_5282 at 6074.015 kb on - strand, within Pf6N2E2_5282 at 6074.082 kb on + strand, within Pf6N2E2_5282 at 6074.082 kb on + strand, within Pf6N2E2_5282 at 6074.083 kb on - strand, within Pf6N2E2_5282 at 6074.126 kb on + strand, within Pf6N2E2_5282 at 6074.126 kb on + strand, within Pf6N2E2_5282 at 6074.126 kb on + strand, within Pf6N2E2_5282 at 6074.126 kb on + strand, within Pf6N2E2_5282 at 6074.127 kb on - strand, within Pf6N2E2_5282 at 6074.358 kb on - strand at 6074.422 kb on - strand at 6074.513 kb on + strand, within Pf6N2E2_5283 at 6074.514 kb on - strand, within Pf6N2E2_5283 at 6074.559 kb on + strand, within Pf6N2E2_5283 at 6074.560 kb on - strand, within Pf6N2E2_5283 at 6074.560 kb on - strand, within Pf6N2E2_5283 at 6074.560 kb on - strand, within Pf6N2E2_5283 at 6074.565 kb on + strand, within Pf6N2E2_5283 at 6074.565 kb on + strand, within Pf6N2E2_5283 at 6074.565 kb on + strand, within Pf6N2E2_5283 at 6074.566 kb on - strand, within Pf6N2E2_5283 at 6074.566 kb on - strand, within Pf6N2E2_5283 at 6074.619 kb on + strand, within Pf6N2E2_5283 at 6074.619 kb on + strand, within Pf6N2E2_5283 at 6074.620 kb on - strand, within Pf6N2E2_5283 at 6074.620 kb on - strand, within Pf6N2E2_5283 at 6074.631 kb on + strand, within Pf6N2E2_5283 at 6074.631 kb on + strand, within Pf6N2E2_5283 at 6074.632 kb on - strand, within Pf6N2E2_5283 at 6074.632 kb on - strand, within Pf6N2E2_5283 at 6074.676 kb on + strand at 6074.687 kb on + strand at 6074.688 kb on - strand at 6074.688 kb on - strand at 6074.741 kb on - strand at 6074.741 kb on - strand at 6074.741 kb on - strand at 6074.741 kb on - strand at 6074.870 kb on + strand at 6074.870 kb on + strand at 6074.870 kb on + strand at 6074.871 kb on - strand at 6074.871 kb on - strand at 6074.871 kb on - strand at 6074.871 kb on - strand at 6074.871 kb on - strand at 6075.024 kb on + strand at 6075.024 kb on + strand at 6075.024 kb on + strand at 6075.034 kb on + strand at 6075.035 kb on - strand at 6075.035 kb on - strand at 6075.035 kb on - strand at 6075.038 kb on + strand at 6075.049 kb on + strand at 6075.050 kb on - strand at 6075.134 kb on + strand at 6075.134 kb on + strand at 6075.134 kb on + strand at 6075.135 kb on - strand at 6075.135 kb on - strand at 6075.135 kb on - strand at 6075.135 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 6,072,533 + Pf6N2E2_5281 0.27 +0.7 6,072,533 + Pf6N2E2_5281 0.27 +1.0 6,072,534 - Pf6N2E2_5281 0.27 -0.6 6,072,620 + Pf6N2E2_5281 0.36 -0.0 6,072,620 + Pf6N2E2_5281 0.36 -1.0 6,072,628 + Pf6N2E2_5281 0.37 -2.6 6,072,660 - Pf6N2E2_5281 0.41 +1.0 6,072,660 - Pf6N2E2_5281 0.41 -0.6 6,072,845 - Pf6N2E2_5281 0.61 -0.0 6,072,858 + Pf6N2E2_5281 0.63 +1.0 6,072,858 + Pf6N2E2_5281 0.63 -1.6 6,072,858 + Pf6N2E2_5281 0.63 -0.6 6,072,858 + Pf6N2E2_5281 0.63 +1.0 6,072,859 - Pf6N2E2_5281 0.63 -0.0 6,072,859 - Pf6N2E2_5281 0.63 -0.6 6,072,923 + Pf6N2E2_5281 0.70 -2.3 6,072,923 + Pf6N2E2_5281 0.70 -0.8 6,072,923 + Pf6N2E2_5281 0.70 -0.6 6,072,923 + Pf6N2E2_5281 0.70 -0.9 6,072,923 + Pf6N2E2_5281 0.70 +1.0 6,072,923 + Pf6N2E2_5281 0.70 -0.6 6,072,923 + Pf6N2E2_5281 0.70 +1.0 6,072,923 + Pf6N2E2_5281 0.70 -0.0 6,072,924 - Pf6N2E2_5281 0.70 +1.0 6,072,924 - Pf6N2E2_5281 0.70 -0.6 6,072,924 - Pf6N2E2_5281 0.70 -0.6 6,072,924 - Pf6N2E2_5281 0.70 -0.9 6,072,924 - Pf6N2E2_5281 0.70 -0.3 6,072,924 - Pf6N2E2_5281 0.70 +1.0 6,072,924 - Pf6N2E2_5281 0.70 +1.0 6,072,924 - Pf6N2E2_5281 0.70 -0.6 6,072,924 - Pf6N2E2_5281 0.70 -0.7 6,072,924 - Pf6N2E2_5281 0.70 +1.0 6,072,926 - Pf6N2E2_5281 0.70 -1.0 6,073,139 + +1.0 6,073,139 + +1.0 6,073,140 - -0.0 6,073,140 - +1.0 6,073,140 - +1.0 6,073,199 + -1.5 6,073,200 - +1.0 6,073,250 - -0.0 6,073,250 - +0.1 6,073,250 - -0.3 6,073,250 - +1.0 6,073,250 - -1.6 6,073,250 - -0.6 6,073,704 + Pf6N2E2_5282 0.27 +1.0 6,073,705 - Pf6N2E2_5282 0.27 -2.1 6,073,815 - Pf6N2E2_5282 0.39 -0.0 6,073,839 - Pf6N2E2_5282 0.42 -0.0 6,073,839 - Pf6N2E2_5282 0.42 -0.0 6,073,839 - Pf6N2E2_5282 0.42 -1.0 6,073,839 - Pf6N2E2_5282 0.42 +1.0 6,073,839 - Pf6N2E2_5282 0.42 -0.0 6,073,839 - Pf6N2E2_5282 0.42 +1.0 6,073,839 - Pf6N2E2_5282 0.42 -0.6 6,073,922 + Pf6N2E2_5282 0.51 +1.0 6,073,923 - Pf6N2E2_5282 0.51 -0.3 6,073,923 - Pf6N2E2_5282 0.51 -0.0 6,073,923 - Pf6N2E2_5282 0.51 -0.6 6,073,923 - Pf6N2E2_5282 0.51 +0.3 6,073,947 - Pf6N2E2_5282 0.54 +1.0 6,073,970 + Pf6N2E2_5282 0.56 -1.0 6,073,971 - Pf6N2E2_5282 0.56 -0.0 6,073,971 - Pf6N2E2_5282 0.56 -0.6 6,074,014 + Pf6N2E2_5282 0.61 -0.6 6,074,014 + Pf6N2E2_5282 0.61 -2.0 6,074,015 - Pf6N2E2_5282 0.61 -1.0 6,074,015 - Pf6N2E2_5282 0.61 -0.0 6,074,082 + Pf6N2E2_5282 0.69 -0.0 6,074,082 + Pf6N2E2_5282 0.69 +1.0 6,074,083 - Pf6N2E2_5282 0.69 -1.0 6,074,126 + Pf6N2E2_5282 0.73 -0.5 6,074,126 + Pf6N2E2_5282 0.73 -0.0 6,074,126 + Pf6N2E2_5282 0.73 +1.0 6,074,126 + Pf6N2E2_5282 0.73 +1.0 6,074,127 - Pf6N2E2_5282 0.73 -0.6 6,074,358 - -0.7 6,074,422 - +1.0 6,074,513 + Pf6N2E2_5283 0.35 -0.0 6,074,514 - Pf6N2E2_5283 0.35 -0.6 6,074,559 + Pf6N2E2_5283 0.50 +1.0 6,074,560 - Pf6N2E2_5283 0.51 +1.0 6,074,560 - Pf6N2E2_5283 0.51 -0.0 6,074,560 - Pf6N2E2_5283 0.51 -0.6 6,074,565 + Pf6N2E2_5283 0.52 -0.9 6,074,565 + Pf6N2E2_5283 0.52 -0.0 6,074,565 + Pf6N2E2_5283 0.52 +1.0 6,074,566 - Pf6N2E2_5283 0.53 -0.5 6,074,566 - Pf6N2E2_5283 0.53 -0.0 6,074,619 + Pf6N2E2_5283 0.71 -1.6 6,074,619 + Pf6N2E2_5283 0.71 +1.0 6,074,620 - Pf6N2E2_5283 0.71 -0.0 6,074,620 - Pf6N2E2_5283 0.71 +1.0 6,074,631 + Pf6N2E2_5283 0.75 -1.0 6,074,631 + Pf6N2E2_5283 0.75 +1.0 6,074,632 - Pf6N2E2_5283 0.75 -0.6 6,074,632 - Pf6N2E2_5283 0.75 +1.0 6,074,676 + -1.0 6,074,687 + -0.9 6,074,688 - +1.0 6,074,688 - -0.7 6,074,741 - -0.6 6,074,741 - -1.3 6,074,741 - +1.0 6,074,741 - +1.0 6,074,870 + -0.1 6,074,870 + +1.0 6,074,870 + +1.0 6,074,871 - +1.0 6,074,871 - -1.9 6,074,871 - +1.0 6,074,871 - -0.2 6,074,871 - -1.1 6,075,024 + +1.0 6,075,024 + -0.4 6,075,024 + +1.0 6,075,034 + -0.0 6,075,035 - +1.0 6,075,035 - -0.0 6,075,035 - +1.0 6,075,038 + -0.9 6,075,049 + +1.0 6,075,050 - -0.0 6,075,134 + +1.0 6,075,134 + +1.0 6,075,134 + +1.0 6,075,135 - -0.7 6,075,135 - +1.0 6,075,135 - -2.0 6,075,135 - -0.0
Or see this region's nucleotide sequence