Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_495

Experiment: Ying_Nucleobase14 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_493 and Pf6N2E2_494 are separated by 149 nucleotidesPf6N2E2_494 and Pf6N2E2_495 are separated by 29 nucleotidesPf6N2E2_495 and Pf6N2E2_496 are separated by 183 nucleotides Pf6N2E2_493 - YD repeat protein, at 559,785 to 560,831 _493 Pf6N2E2_494 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 560,981 to 561,418 _494 Pf6N2E2_495 - YD repeat protein, at 561,448 to 562,563 _495 Pf6N2E2_496 - Dihydropyrimidine dehydrogenase (NADP(+)) (EC 1.3.1.2) (from data), at 562,747 to 564,021 _496 Position (kb) 561 562 563Strain fitness (log2 ratio) -2 -1 0 1 2at 560.452 kb on + strand, within Pf6N2E2_493at 560.452 kb on + strand, within Pf6N2E2_493at 560.701 kb on - strand, within Pf6N2E2_493at 560.701 kb on - strand, within Pf6N2E2_493at 560.701 kb on - strand, within Pf6N2E2_493at 560.701 kb on - strand, within Pf6N2E2_493at 560.729 kb on - strandat 560.729 kb on - strandat 560.729 kb on - strandat 560.894 kb on - strandat 560.896 kb on + strandat 560.908 kb on + strandat 560.909 kb on - strandat 560.909 kb on - strandat 560.927 kb on - strandat 560.946 kb on + strandat 560.946 kb on + strandat 560.947 kb on - strandat 560.947 kb on - strandat 560.947 kb on - strandat 560.947 kb on - strandat 560.947 kb on - strandat 560.949 kb on + strandat 560.949 kb on + strandat 560.949 kb on + strandat 560.950 kb on - strandat 560.950 kb on - strandat 560.971 kb on + strandat 560.972 kb on - strandat 560.972 kb on - strandat 560.972 kb on - strandat 560.972 kb on - strandat 560.973 kb on + strandat 560.973 kb on + strandat 561.065 kb on + strand, within Pf6N2E2_494at 561.134 kb on - strand, within Pf6N2E2_494at 561.134 kb on - strand, within Pf6N2E2_494at 561.134 kb on - strand, within Pf6N2E2_494at 561.180 kb on - strand, within Pf6N2E2_494at 561.453 kb on - strandat 561.573 kb on + strand, within Pf6N2E2_495at 561.637 kb on + strand, within Pf6N2E2_495at 561.834 kb on + strand, within Pf6N2E2_495at 562.001 kb on + strand, within Pf6N2E2_495at 562.085 kb on + strand, within Pf6N2E2_495at 562.184 kb on + strand, within Pf6N2E2_495at 562.184 kb on + strand, within Pf6N2E2_495at 562.607 kb on + strandat 562.625 kb on + strandat 562.628 kb on + strandat 562.640 kb on + strandat 562.640 kb on + strandat 562.640 kb on + strandat 562.640 kb on + strandat 562.641 kb on - strandat 562.764 kb on - strandat 562.867 kb on - strandat 562.892 kb on - strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.899 kb on - strand, within Pf6N2E2_496at 562.902 kb on + strand, within Pf6N2E2_496at 562.902 kb on + strand, within Pf6N2E2_496at 562.902 kb on + strand, within Pf6N2E2_496at 562.903 kb on - strandat 562.903 kb on - strand, within Pf6N2E2_496at 562.903 kb on - strand, within Pf6N2E2_496at 562.903 kb on - strand, within Pf6N2E2_496at 562.903 kb on - strand, within Pf6N2E2_496at 562.903 kb on - strand, within Pf6N2E2_496at 563.021 kb on + strand, within Pf6N2E2_496at 563.021 kb on + strand, within Pf6N2E2_496at 563.022 kb on - strand, within Pf6N2E2_496at 563.022 kb on - strand, within Pf6N2E2_496at 563.022 kb on - strand, within Pf6N2E2_496at 563.036 kb on + strand, within Pf6N2E2_496at 563.036 kb on + strand, within Pf6N2E2_496at 563.036 kb on + strand, within Pf6N2E2_496at 563.522 kb on + strand, within Pf6N2E2_496at 563.522 kb on + strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.555 kb on + strand, within Pf6N2E2_496at 563.555 kb on + strand, within Pf6N2E2_496at 563.556 kb on - strand, within Pf6N2E2_496at 563.556 kb on - strand, within Pf6N2E2_496

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2
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560,452 + Pf6N2E2_493 0.64 +0.9
560,452 + Pf6N2E2_493 0.64 -0.1
560,701 - Pf6N2E2_493 0.87 +0.9
560,701 - Pf6N2E2_493 0.87 +0.9
560,701 - Pf6N2E2_493 0.87 -0.3
560,701 - Pf6N2E2_493 0.87 +0.9
560,729 - -0.6
560,729 - +0.1
560,729 - -0.1
560,894 - +0.9
560,896 + +0.9
560,908 + -0.1
560,909 - -0.1
560,909 - -1.5
560,927 - +0.9
560,946 + +0.9
560,946 + +0.9
560,947 - -0.7
560,947 - -0.1
560,947 - +0.9
560,947 - -1.0
560,947 - -0.1
560,949 + +0.9
560,949 + +0.9
560,949 + +0.9
560,950 - -0.5
560,950 - +0.9
560,971 + +0.9
560,972 - -0.1
560,972 - +0.9
560,972 - -0.1
560,972 - +0.9
560,973 + +0.9
560,973 + +0.9
561,065 + Pf6N2E2_494 0.19 +0.9
561,134 - Pf6N2E2_494 0.35 +0.9
561,134 - Pf6N2E2_494 0.35 +0.9
561,134 - Pf6N2E2_494 0.35 -0.7
561,180 - Pf6N2E2_494 0.45 +0.9
561,453 - +0.9
561,573 + Pf6N2E2_495 0.11 +0.4
561,637 + Pf6N2E2_495 0.17 +0.8
561,834 + Pf6N2E2_495 0.35 +0.8
562,001 + Pf6N2E2_495 0.50 -0.2
562,085 + Pf6N2E2_495 0.57 +0.8
562,184 + Pf6N2E2_495 0.66 +0.8
562,184 + Pf6N2E2_495 0.66 +0.8
562,607 + -0.2
562,625 + -0.2
562,628 + +0.8
562,640 + +0.8
562,640 + +0.8
562,640 + +0.8
562,640 + +0.8
562,641 - +0.8
562,764 - -0.2
562,867 - -1.0
562,892 - Pf6N2E2_496 0.11 -0.2
562,898 + Pf6N2E2_496 0.12 +0.8
562,898 + Pf6N2E2_496 0.12 +0.8
562,898 + Pf6N2E2_496 0.12 -0.2
562,898 + Pf6N2E2_496 0.12 +0.8
562,898 + Pf6N2E2_496 0.12 -0.7
562,898 + Pf6N2E2_496 0.12 +0.8
562,899 - Pf6N2E2_496 0.12 +0.8
562,902 + Pf6N2E2_496 0.12 -0.2
562,902 + Pf6N2E2_496 0.12 -0.9
562,902 + Pf6N2E2_496 0.12 +0.8
562,903 - +0.8
562,903 - Pf6N2E2_496 0.12 +0.8
562,903 - Pf6N2E2_496 0.12 +0.8
562,903 - Pf6N2E2_496 0.12 -0.2
562,903 - Pf6N2E2_496 0.12 -0.2
562,903 - Pf6N2E2_496 0.12 +0.8
563,021 + Pf6N2E2_496 0.21 +0.8
563,021 + Pf6N2E2_496 0.21 +0.8
563,022 - Pf6N2E2_496 0.22 -2.0
563,022 - Pf6N2E2_496 0.22 +0.8
563,022 - Pf6N2E2_496 0.22 -1.2
563,036 + Pf6N2E2_496 0.23 +0.8
563,036 + Pf6N2E2_496 0.23 -1.2
563,036 + Pf6N2E2_496 0.23 +0.8
563,522 + Pf6N2E2_496 0.61 +0.8
563,522 + Pf6N2E2_496 0.61 +0.8
563,523 - Pf6N2E2_496 0.61 +1.8
563,523 - Pf6N2E2_496 0.61 +0.8
563,523 - Pf6N2E2_496 0.61 -0.4
563,523 - Pf6N2E2_496 0.61 +0.8
563,523 - Pf6N2E2_496 0.61 +0.0
563,523 - Pf6N2E2_496 0.61 +0.8
563,555 + Pf6N2E2_496 0.63 -1.0
563,555 + Pf6N2E2_496 0.63 +0.8
563,556 - Pf6N2E2_496 0.63 +0.8
563,556 - Pf6N2E2_496 0.63 -0.2

Or see this region's nucleotide sequence