Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4168 and Pf6N2E2_4169 are separated by 37 nucleotides Pf6N2E2_4169 and Pf6N2E2_4170 are separated by 101 nucleotides Pf6N2E2_4170 and Pf6N2E2_4171 are separated by 180 nucleotides Pf6N2E2_4171 and Pf6N2E2_4172 are separated by 21 nucleotides
Pf6N2E2_4168 - Zinc ABC transporter, inner membrane permease protein ZnuB, at 4,849,877 to 4,850,665
_4168
Pf6N2E2_4169 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 4,850,703 to 4,851,416
_4169
Pf6N2E2_4170 - Catalase (EC 1.11.1.6), at 4,851,518 to 4,853,656
_4170
Pf6N2E2_4171 - hypothetical protein, at 4,853,837 to 4,854,037
_4171
Pf6N2E2_4172 - Methionine ABC transporter ATP-binding protein, at 4,854,059 to 4,854,928
_4172
Position (kb)
4851
4852
4853
4854 Strain fitness (log2 ratio)
-1
0
1
2 at 4850.647 kb on - strand at 4850.663 kb on + strand at 4850.663 kb on + strand at 4850.663 kb on + strand at 4850.688 kb on + strand at 4850.688 kb on + strand at 4850.688 kb on + strand at 4850.688 kb on + strand at 4850.771 kb on + strand at 4850.772 kb on - strand at 4850.919 kb on + strand, within Pf6N2E2_4169 at 4850.943 kb on + strand, within Pf6N2E2_4169 at 4850.943 kb on + strand, within Pf6N2E2_4169 at 4850.944 kb on - strand, within Pf6N2E2_4169 at 4850.944 kb on - strand, within Pf6N2E2_4169 at 4850.944 kb on - strand, within Pf6N2E2_4169 at 4851.188 kb on - strand, within Pf6N2E2_4169 at 4851.196 kb on + strand, within Pf6N2E2_4169 at 4851.196 kb on + strand, within Pf6N2E2_4169 at 4851.196 kb on + strand, within Pf6N2E2_4169 at 4851.246 kb on + strand, within Pf6N2E2_4169 at 4851.247 kb on - strand, within Pf6N2E2_4169 at 4851.247 kb on - strand, within Pf6N2E2_4169 at 4851.247 kb on - strand, within Pf6N2E2_4169 at 4851.247 kb on - strand, within Pf6N2E2_4169 at 4851.289 kb on - strand, within Pf6N2E2_4169 at 4851.407 kb on + strand at 4851.407 kb on + strand at 4851.407 kb on + strand at 4851.407 kb on + strand at 4851.407 kb on + strand at 4851.408 kb on - strand at 4851.414 kb on + strand at 4851.414 kb on + strand at 4851.535 kb on + strand at 4851.542 kb on - strand at 4851.702 kb on + strand at 4851.702 kb on + strand at 4851.703 kb on - strand at 4851.703 kb on - strand at 4851.717 kb on + strand at 4851.717 kb on + strand at 4851.717 kb on + strand at 4851.717 kb on + strand at 4851.717 kb on + strand at 4851.717 kb on + strand at 4851.718 kb on - strand at 4851.718 kb on - strand at 4851.718 kb on - strand at 4852.063 kb on + strand, within Pf6N2E2_4170 at 4852.118 kb on - strand, within Pf6N2E2_4170 at 4852.188 kb on + strand, within Pf6N2E2_4170 at 4852.233 kb on + strand, within Pf6N2E2_4170 at 4852.233 kb on + strand, within Pf6N2E2_4170 at 4852.233 kb on + strand, within Pf6N2E2_4170 at 4852.233 kb on + strand, within Pf6N2E2_4170 at 4852.233 kb on + strand, within Pf6N2E2_4170 at 4852.234 kb on - strand, within Pf6N2E2_4170 at 4852.234 kb on - strand, within Pf6N2E2_4170 at 4852.293 kb on + strand, within Pf6N2E2_4170 at 4852.293 kb on + strand, within Pf6N2E2_4170 at 4852.294 kb on - strand, within Pf6N2E2_4170 at 4852.294 kb on - strand, within Pf6N2E2_4170 at 4852.294 kb on - strand, within Pf6N2E2_4170 at 4852.389 kb on + strand, within Pf6N2E2_4170 at 4852.583 kb on - strand, within Pf6N2E2_4170 at 4852.583 kb on - strand, within Pf6N2E2_4170 at 4852.583 kb on - strand, within Pf6N2E2_4170 at 4852.583 kb on - strand, within Pf6N2E2_4170 at 4852.726 kb on + strand, within Pf6N2E2_4170 at 4852.726 kb on + strand, within Pf6N2E2_4170 at 4852.726 kb on + strand, within Pf6N2E2_4170 at 4852.726 kb on + strand, within Pf6N2E2_4170 at 4852.726 kb on + strand, within Pf6N2E2_4170 at 4852.726 kb on + strand, within Pf6N2E2_4170 at 4852.727 kb on - strand, within Pf6N2E2_4170 at 4852.727 kb on - strand, within Pf6N2E2_4170 at 4852.727 kb on - strand, within Pf6N2E2_4170 at 4852.727 kb on - strand, within Pf6N2E2_4170 at 4852.727 kb on - strand, within Pf6N2E2_4170 at 4852.727 kb on - strand, within Pf6N2E2_4170 at 4852.727 kb on - strand, within Pf6N2E2_4170 at 4852.727 kb on - strand, within Pf6N2E2_4170 at 4852.727 kb on - strand, within Pf6N2E2_4170 at 4852.727 kb on - strand, within Pf6N2E2_4170 at 4852.727 kb on - strand, within Pf6N2E2_4170 at 4852.942 kb on + strand, within Pf6N2E2_4170 at 4852.942 kb on + strand, within Pf6N2E2_4170 at 4852.942 kb on + strand, within Pf6N2E2_4170 at 4852.942 kb on + strand, within Pf6N2E2_4170 at 4852.942 kb on + strand, within Pf6N2E2_4170 at 4852.942 kb on + strand, within Pf6N2E2_4170 at 4852.943 kb on - strand, within Pf6N2E2_4170 at 4852.943 kb on - strand, within Pf6N2E2_4170 at 4852.943 kb on - strand, within Pf6N2E2_4170 at 4852.943 kb on - strand, within Pf6N2E2_4170 at 4852.943 kb on - strand, within Pf6N2E2_4170 at 4852.943 kb on - strand, within Pf6N2E2_4170 at 4852.943 kb on - strand, within Pf6N2E2_4170 at 4852.970 kb on + strand, within Pf6N2E2_4170 at 4852.970 kb on + strand, within Pf6N2E2_4170 at 4853.014 kb on + strand, within Pf6N2E2_4170 at 4853.014 kb on + strand, within Pf6N2E2_4170 at 4853.014 kb on + strand, within Pf6N2E2_4170 at 4853.014 kb on + strand, within Pf6N2E2_4170 at 4853.014 kb on + strand, within Pf6N2E2_4170 at 4853.014 kb on + strand, within Pf6N2E2_4170 at 4853.014 kb on + strand, within Pf6N2E2_4170 at 4853.014 kb on + strand, within Pf6N2E2_4170 at 4853.014 kb on + strand, within Pf6N2E2_4170 at 4853.014 kb on + strand, within Pf6N2E2_4170 at 4853.014 kb on + strand, within Pf6N2E2_4170 at 4853.015 kb on - strand, within Pf6N2E2_4170 at 4853.015 kb on - strand, within Pf6N2E2_4170 at 4853.015 kb on - strand, within Pf6N2E2_4170 at 4853.015 kb on - strand, within Pf6N2E2_4170 at 4853.015 kb on - strand, within Pf6N2E2_4170 at 4853.015 kb on - strand, within Pf6N2E2_4170 at 4853.015 kb on - strand, within Pf6N2E2_4170 at 4853.015 kb on - strand, within Pf6N2E2_4170 at 4853.185 kb on + strand, within Pf6N2E2_4170 at 4853.186 kb on - strand, within Pf6N2E2_4170 at 4853.186 kb on - strand, within Pf6N2E2_4170 at 4853.310 kb on - strand, within Pf6N2E2_4170 at 4853.346 kb on + strand, within Pf6N2E2_4170 at 4853.346 kb on + strand, within Pf6N2E2_4170 at 4853.346 kb on + strand, within Pf6N2E2_4170 at 4853.347 kb on - strand, within Pf6N2E2_4170 at 4853.347 kb on - strand, within Pf6N2E2_4170 at 4853.347 kb on - strand, within Pf6N2E2_4170 at 4853.347 kb on - strand, within Pf6N2E2_4170 at 4853.347 kb on - strand, within Pf6N2E2_4170 at 4853.358 kb on + strand, within Pf6N2E2_4170 at 4853.358 kb on + strand, within Pf6N2E2_4170 at 4853.359 kb on - strand, within Pf6N2E2_4170 at 4853.359 kb on - strand, within Pf6N2E2_4170 at 4853.359 kb on - strand, within Pf6N2E2_4170 at 4853.359 kb on - strand, within Pf6N2E2_4170 at 4853.650 kb on + strand at 4853.650 kb on + strand at 4853.746 kb on - strand at 4853.784 kb on + strand at 4853.784 kb on + strand at 4853.785 kb on - strand at 4853.832 kb on - strand at 4853.832 kb on - strand at 4853.838 kb on + strand at 4853.839 kb on - strand at 4853.861 kb on + strand, within Pf6N2E2_4171 at 4853.861 kb on + strand, within Pf6N2E2_4171 at 4853.862 kb on - strand, within Pf6N2E2_4171 at 4853.919 kb on - strand, within Pf6N2E2_4171 at 4853.919 kb on - strand, within Pf6N2E2_4171 at 4853.944 kb on + strand, within Pf6N2E2_4171 at 4853.944 kb on + strand, within Pf6N2E2_4171 at 4854.332 kb on + strand, within Pf6N2E2_4172 at 4854.515 kb on - strand, within Pf6N2E2_4172
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 4,850,647 - -0.5 4,850,663 + +0.1 4,850,663 + +1.1 4,850,663 + -1.2 4,850,688 + +1.1 4,850,688 + -0.5 4,850,688 + +0.3 4,850,688 + +1.1 4,850,771 + -0.3 4,850,772 - -0.3 4,850,919 + Pf6N2E2_4169 0.30 -0.8 4,850,943 + Pf6N2E2_4169 0.34 -0.5 4,850,943 + Pf6N2E2_4169 0.34 +1.1 4,850,944 - Pf6N2E2_4169 0.34 +1.1 4,850,944 - Pf6N2E2_4169 0.34 +1.1 4,850,944 - Pf6N2E2_4169 0.34 -1.1 4,851,188 - Pf6N2E2_4169 0.68 +1.1 4,851,196 + Pf6N2E2_4169 0.69 -0.6 4,851,196 + Pf6N2E2_4169 0.69 +0.1 4,851,196 + Pf6N2E2_4169 0.69 +0.1 4,851,246 + Pf6N2E2_4169 0.76 -1.2 4,851,247 - Pf6N2E2_4169 0.76 -0.5 4,851,247 - Pf6N2E2_4169 0.76 +1.1 4,851,247 - Pf6N2E2_4169 0.76 -0.5 4,851,247 - Pf6N2E2_4169 0.76 -1.4 4,851,289 - Pf6N2E2_4169 0.82 +1.1 4,851,407 + +1.1 4,851,407 + +1.1 4,851,407 + +1.1 4,851,407 + +0.1 4,851,407 + -0.5 4,851,408 - -0.5 4,851,414 + +1.1 4,851,414 + +1.1 4,851,535 + +1.1 4,851,542 - +0.1 4,851,702 + -0.6 4,851,702 + +0.1 4,851,703 - -0.8 4,851,703 - +0.1 4,851,717 + -0.9 4,851,717 + -0.6 4,851,717 + +1.1 4,851,717 + -0.5 4,851,717 + +1.1 4,851,717 + -0.2 4,851,718 - -0.5 4,851,718 - -0.5 4,851,718 - +1.1 4,852,063 + Pf6N2E2_4170 0.25 +1.1 4,852,118 - Pf6N2E2_4170 0.28 -0.5 4,852,188 + Pf6N2E2_4170 0.31 -0.5 4,852,233 + Pf6N2E2_4170 0.33 +1.1 4,852,233 + Pf6N2E2_4170 0.33 -0.6 4,852,233 + Pf6N2E2_4170 0.33 +1.1 4,852,233 + Pf6N2E2_4170 0.33 -0.5 4,852,233 + Pf6N2E2_4170 0.33 +1.1 4,852,234 - Pf6N2E2_4170 0.33 -0.9 4,852,234 - Pf6N2E2_4170 0.33 +0.1 4,852,293 + Pf6N2E2_4170 0.36 +1.1 4,852,293 + Pf6N2E2_4170 0.36 +1.1 4,852,294 - Pf6N2E2_4170 0.36 +1.1 4,852,294 - Pf6N2E2_4170 0.36 +1.1 4,852,294 - Pf6N2E2_4170 0.36 +0.1 4,852,389 + Pf6N2E2_4170 0.41 +0.1 4,852,583 - Pf6N2E2_4170 0.50 +1.1 4,852,583 - Pf6N2E2_4170 0.50 -1.4 4,852,583 - Pf6N2E2_4170 0.50 +2.1 4,852,583 - Pf6N2E2_4170 0.50 -1.3 4,852,726 + Pf6N2E2_4170 0.56 -0.5 4,852,726 + Pf6N2E2_4170 0.56 +0.1 4,852,726 + Pf6N2E2_4170 0.56 -0.8 4,852,726 + Pf6N2E2_4170 0.56 +1.1 4,852,726 + Pf6N2E2_4170 0.56 +1.1 4,852,726 + Pf6N2E2_4170 0.56 +1.1 4,852,727 - Pf6N2E2_4170 0.57 +0.1 4,852,727 - Pf6N2E2_4170 0.57 -0.5 4,852,727 - Pf6N2E2_4170 0.57 -0.7 4,852,727 - Pf6N2E2_4170 0.57 +0.1 4,852,727 - Pf6N2E2_4170 0.57 -0.9 4,852,727 - Pf6N2E2_4170 0.57 +1.1 4,852,727 - Pf6N2E2_4170 0.57 +0.1 4,852,727 - Pf6N2E2_4170 0.57 -1.0 4,852,727 - Pf6N2E2_4170 0.57 -0.5 4,852,727 - Pf6N2E2_4170 0.57 -1.2 4,852,727 - Pf6N2E2_4170 0.57 +0.6 4,852,942 + Pf6N2E2_4170 0.67 +0.1 4,852,942 + Pf6N2E2_4170 0.67 -1.4 4,852,942 + Pf6N2E2_4170 0.67 -1.4 4,852,942 + Pf6N2E2_4170 0.67 +1.1 4,852,942 + Pf6N2E2_4170 0.67 +0.2 4,852,942 + Pf6N2E2_4170 0.67 -0.5 4,852,943 - Pf6N2E2_4170 0.67 +0.1 4,852,943 - Pf6N2E2_4170 0.67 -0.5 4,852,943 - Pf6N2E2_4170 0.67 +0.1 4,852,943 - Pf6N2E2_4170 0.67 +0.1 4,852,943 - Pf6N2E2_4170 0.67 -0.0 4,852,943 - Pf6N2E2_4170 0.67 -0.3 4,852,943 - Pf6N2E2_4170 0.67 -1.0 4,852,970 + Pf6N2E2_4170 0.68 +0.1 4,852,970 + Pf6N2E2_4170 0.68 +0.1 4,853,014 + Pf6N2E2_4170 0.70 +1.1 4,853,014 + Pf6N2E2_4170 0.70 +0.1 4,853,014 + Pf6N2E2_4170 0.70 +1.1 4,853,014 + Pf6N2E2_4170 0.70 -0.5 4,853,014 + Pf6N2E2_4170 0.70 +1.1 4,853,014 + Pf6N2E2_4170 0.70 +0.1 4,853,014 + Pf6N2E2_4170 0.70 +1.1 4,853,014 + Pf6N2E2_4170 0.70 +0.1 4,853,014 + Pf6N2E2_4170 0.70 +0.1 4,853,014 + Pf6N2E2_4170 0.70 -1.1 4,853,014 + Pf6N2E2_4170 0.70 +1.1 4,853,015 - Pf6N2E2_4170 0.70 +0.1 4,853,015 - Pf6N2E2_4170 0.70 +0.1 4,853,015 - Pf6N2E2_4170 0.70 -0.8 4,853,015 - Pf6N2E2_4170 0.70 +0.1 4,853,015 - Pf6N2E2_4170 0.70 -0.9 4,853,015 - Pf6N2E2_4170 0.70 -0.6 4,853,015 - Pf6N2E2_4170 0.70 -0.2 4,853,015 - Pf6N2E2_4170 0.70 +0.5 4,853,185 + Pf6N2E2_4170 0.78 +0.1 4,853,186 - Pf6N2E2_4170 0.78 +0.1 4,853,186 - Pf6N2E2_4170 0.78 -0.8 4,853,310 - Pf6N2E2_4170 0.84 +1.1 4,853,346 + Pf6N2E2_4170 0.85 -1.2 4,853,346 + Pf6N2E2_4170 0.85 +0.1 4,853,346 + Pf6N2E2_4170 0.85 +1.1 4,853,347 - Pf6N2E2_4170 0.86 +0.1 4,853,347 - Pf6N2E2_4170 0.86 +0.1 4,853,347 - Pf6N2E2_4170 0.86 +0.2 4,853,347 - Pf6N2E2_4170 0.86 -1.5 4,853,347 - Pf6N2E2_4170 0.86 +0.1 4,853,358 + Pf6N2E2_4170 0.86 +1.1 4,853,358 + Pf6N2E2_4170 0.86 -0.5 4,853,359 - Pf6N2E2_4170 0.86 +1.1 4,853,359 - Pf6N2E2_4170 0.86 +0.1 4,853,359 - Pf6N2E2_4170 0.86 +1.1 4,853,359 - Pf6N2E2_4170 0.86 -1.7 4,853,650 + +0.1 4,853,650 + -1.5 4,853,746 - +1.1 4,853,784 + +1.1 4,853,784 + +0.1 4,853,785 - +0.1 4,853,832 - +1.1 4,853,832 - +1.1 4,853,838 + -0.5 4,853,839 - -0.2 4,853,861 + Pf6N2E2_4171 0.12 -1.7 4,853,861 + Pf6N2E2_4171 0.12 -0.9 4,853,862 - Pf6N2E2_4171 0.12 +0.1 4,853,919 - Pf6N2E2_4171 0.41 +1.1 4,853,919 - Pf6N2E2_4171 0.41 +1.1 4,853,944 + Pf6N2E2_4171 0.53 +1.1 4,853,944 + Pf6N2E2_4171 0.53 +1.1 4,854,332 + Pf6N2E2_4172 0.31 -0.7 4,854,515 - Pf6N2E2_4172 0.52 +1.1
Or see this region's nucleotide sequence