Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_164 and Pf6N2E2_165 are separated by 23 nucleotides Pf6N2E2_165 and Pf6N2E2_166 overlap by 4 nucleotides Pf6N2E2_166 and Pf6N2E2_167 are separated by 30 nucleotides
Pf6N2E2_164 - Ribose operon repressor, at 217,827 to 218,846
_164
Pf6N2E2_165 - Ribokinase (EC 2.7.1.15), at 218,870 to 219,784
_165
Pf6N2E2_166 - Ribose ABC transport system, high affinity permease RbsD (TC 3.A.1.2.1), at 219,781 to 220,185
_166
Pf6N2E2_167 - Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1), at 220,216 to 221,244
_167
Position (kb) 219
220
221 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.790 kb on - strand at 218.790 kb on - strand at 218.790 kb on - strand at 218.861 kb on + strand at 218.861 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.869 kb on + strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.870 kb on - strand at 218.883 kb on + strand at 218.883 kb on + strand at 218.883 kb on + strand at 218.883 kb on + strand at 218.884 kb on - strand at 218.890 kb on - strand at 218.892 kb on + strand at 218.892 kb on + strand at 218.892 kb on + strand at 219.377 kb on - strand, within Pf6N2E2_165 at 219.395 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.765 kb on + strand at 219.766 kb on - strand at 219.766 kb on - strand at 219.766 kb on - strand at 220.184 kb on - strand at 220.184 kb on - strand at 220.184 kb on - strand at 220.226 kb on - strand at 220.393 kb on - strand, within Pf6N2E2_167 at 220.393 kb on - strand, within Pf6N2E2_167 at 220.393 kb on - strand, within Pf6N2E2_167 at 220.393 kb on - strand, within Pf6N2E2_167 at 220.495 kb on + strand, within Pf6N2E2_167 at 220.496 kb on - strand, within Pf6N2E2_167 at 220.580 kb on + strand, within Pf6N2E2_167 at 220.581 kb on - strand, within Pf6N2E2_167 at 220.581 kb on - strand, within Pf6N2E2_167 at 220.624 kb on + strand, within Pf6N2E2_167 at 220.785 kb on + strand, within Pf6N2E2_167 at 220.785 kb on + strand, within Pf6N2E2_167 at 220.867 kb on + strand, within Pf6N2E2_167 at 220.978 kb on + strand, within Pf6N2E2_167 at 220.978 kb on + strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 221.002 kb on + strand, within Pf6N2E2_167 at 221.002 kb on + strand, within Pf6N2E2_167 at 221.144 kb on - strand at 221.144 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 218,789 + -0.1 218,789 + -0.1 218,789 + -0.1 218,789 + +0.9 218,789 + -0.7 218,789 + +1.1 218,789 + +0.9 218,789 + +1.3 218,789 + +0.9 218,789 + -0.1 218,790 - +0.9 218,790 - -3.5 218,790 - -3.6 218,861 + +0.9 218,861 + +0.9 218,869 + -0.7 218,869 + -0.7 218,869 + -0.1 218,869 + -2.6 218,869 + +0.9 218,869 + -0.1 218,869 + -0.1 218,869 + +0.9 218,869 + -0.9 218,869 + +0.9 218,870 - -1.4 218,870 - +0.9 218,870 - -2.7 218,870 - +0.9 218,870 - +0.9 218,870 - +0.9 218,870 - +0.9 218,870 - +0.9 218,883 + -3.1 218,883 + -1.1 218,883 + -1.4 218,883 + -2.7 218,884 - +0.9 218,890 - -3.8 218,892 + +0.1 218,892 + -0.1 218,892 + -5.6 219,377 - Pf6N2E2_165 0.55 -0.7 219,395 + Pf6N2E2_165 0.57 -0.1 219,421 + Pf6N2E2_165 0.60 -0.1 219,421 + Pf6N2E2_165 0.60 -0.1 219,421 + Pf6N2E2_165 0.60 +0.9 219,421 + Pf6N2E2_165 0.60 +0.9 219,765 + -0.7 219,766 - -2.3 219,766 - +0.9 219,766 - +0.9 220,184 - -0.7 220,184 - +0.9 220,184 - +0.9 220,226 - -0.1 220,393 - Pf6N2E2_167 0.17 -0.9 220,393 - Pf6N2E2_167 0.17 +0.9 220,393 - Pf6N2E2_167 0.17 +0.9 220,393 - Pf6N2E2_167 0.17 +0.9 220,495 + Pf6N2E2_167 0.27 +0.1 220,496 - Pf6N2E2_167 0.27 +0.9 220,580 + Pf6N2E2_167 0.35 +0.9 220,581 - Pf6N2E2_167 0.35 -0.1 220,581 - Pf6N2E2_167 0.35 +0.9 220,624 + Pf6N2E2_167 0.40 +0.9 220,785 + Pf6N2E2_167 0.55 -0.1 220,785 + Pf6N2E2_167 0.55 +0.9 220,867 + Pf6N2E2_167 0.63 +1.5 220,978 + Pf6N2E2_167 0.74 +0.9 220,978 + Pf6N2E2_167 0.74 +0.3 220,979 - Pf6N2E2_167 0.74 +0.9 220,979 - Pf6N2E2_167 0.74 +0.9 220,979 - Pf6N2E2_167 0.74 -1.0 220,979 - Pf6N2E2_167 0.74 -0.1 220,979 - Pf6N2E2_167 0.74 -0.9 220,979 - Pf6N2E2_167 0.74 +0.9 221,002 + Pf6N2E2_167 0.76 -0.9 221,002 + Pf6N2E2_167 0.76 -0.1 221,144 - +0.9 221,144 - -0.8
Or see this region's nucleotide sequence