Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1565 and Pf6N2E2_1566 overlap by 4 nucleotides Pf6N2E2_1566 and Pf6N2E2_1567 are separated by 39 nucleotides Pf6N2E2_1567 and Pf6N2E2_1568 overlap by 4 nucleotides
Pf6N2E2_1565 - FIG00961164: hypothetical protein, at 1,827,610 to 1,828,500
_1565
Pf6N2E2_1566 - FIG00960770: hypothetical protein, at 1,828,497 to 1,829,270
_1566
Pf6N2E2_1567 - FIG00964846: hypothetical protein, at 1,829,310 to 1,830,194
_1567
Pf6N2E2_1568 - FIG00960890: hypothetical protein, at 1,830,191 to 1,831,420
_1568
Position (kb)
1829
1830
1831 Strain fitness (log2 ratio)
-2
-1
0
1 at 1828.315 kb on + strand, within Pf6N2E2_1565 at 1828.316 kb on - strand, within Pf6N2E2_1565 at 1828.363 kb on - strand, within Pf6N2E2_1565 at 1828.363 kb on - strand, within Pf6N2E2_1565 at 1828.442 kb on + strand at 1828.442 kb on + strand at 1828.512 kb on - strand at 1828.816 kb on + strand, within Pf6N2E2_1566 at 1828.816 kb on + strand, within Pf6N2E2_1566 at 1828.816 kb on + strand, within Pf6N2E2_1566 at 1828.817 kb on - strand, within Pf6N2E2_1566 at 1829.038 kb on + strand, within Pf6N2E2_1566 at 1829.038 kb on + strand, within Pf6N2E2_1566 at 1829.225 kb on - strand at 1829.319 kb on + strand at 1829.457 kb on + strand, within Pf6N2E2_1567 at 1829.457 kb on + strand, within Pf6N2E2_1567 at 1829.457 kb on + strand, within Pf6N2E2_1567 at 1829.457 kb on + strand, within Pf6N2E2_1567 at 1829.743 kb on + strand, within Pf6N2E2_1567 at 1829.743 kb on + strand, within Pf6N2E2_1567 at 1829.743 kb on + strand, within Pf6N2E2_1567 at 1829.744 kb on - strand, within Pf6N2E2_1567 at 1829.744 kb on - strand, within Pf6N2E2_1567 at 1829.744 kb on - strand, within Pf6N2E2_1567 at 1830.231 kb on - strand at 1830.494 kb on + strand, within Pf6N2E2_1568 at 1830.495 kb on - strand, within Pf6N2E2_1568 at 1830.495 kb on - strand, within Pf6N2E2_1568 at 1830.531 kb on - strand, within Pf6N2E2_1568 at 1830.531 kb on - strand, within Pf6N2E2_1568 at 1830.873 kb on - strand, within Pf6N2E2_1568 at 1830.873 kb on - strand, within Pf6N2E2_1568 at 1830.873 kb on - strand, within Pf6N2E2_1568 at 1831.016 kb on + strand, within Pf6N2E2_1568 at 1831.017 kb on - strand, within Pf6N2E2_1568 at 1831.017 kb on - strand, within Pf6N2E2_1568 at 1831.017 kb on - strand, within Pf6N2E2_1568
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 1,828,315 + Pf6N2E2_1565 0.79 +0.0 1,828,316 - Pf6N2E2_1565 0.79 +0.8 1,828,363 - Pf6N2E2_1565 0.85 +0.8 1,828,363 - Pf6N2E2_1565 0.85 -0.2 1,828,442 + +0.2 1,828,442 + +0.8 1,828,512 - +0.4 1,828,816 + Pf6N2E2_1566 0.41 -1.5 1,828,816 + Pf6N2E2_1566 0.41 -0.5 1,828,816 + Pf6N2E2_1566 0.41 -0.8 1,828,817 - Pf6N2E2_1566 0.41 -2.1 1,829,038 + Pf6N2E2_1566 0.70 +0.8 1,829,038 + Pf6N2E2_1566 0.70 -1.2 1,829,225 - -0.6 1,829,319 + -0.2 1,829,457 + Pf6N2E2_1567 0.17 -0.2 1,829,457 + Pf6N2E2_1567 0.17 -0.2 1,829,457 + Pf6N2E2_1567 0.17 +0.8 1,829,457 + Pf6N2E2_1567 0.17 +0.8 1,829,743 + Pf6N2E2_1567 0.49 -2.0 1,829,743 + Pf6N2E2_1567 0.49 +0.8 1,829,743 + Pf6N2E2_1567 0.49 +0.2 1,829,744 - Pf6N2E2_1567 0.49 -0.8 1,829,744 - Pf6N2E2_1567 0.49 -1.5 1,829,744 - Pf6N2E2_1567 0.49 -1.8 1,830,231 - -0.2 1,830,494 + Pf6N2E2_1568 0.25 +0.8 1,830,495 - Pf6N2E2_1568 0.25 +0.8 1,830,495 - Pf6N2E2_1568 0.25 -0.2 1,830,531 - Pf6N2E2_1568 0.28 +0.8 1,830,531 - Pf6N2E2_1568 0.28 -0.9 1,830,873 - Pf6N2E2_1568 0.55 -0.3 1,830,873 - Pf6N2E2_1568 0.55 -0.2 1,830,873 - Pf6N2E2_1568 0.55 +0.8 1,831,016 + Pf6N2E2_1568 0.67 +0.5 1,831,017 - Pf6N2E2_1568 0.67 +0.8 1,831,017 - Pf6N2E2_1568 0.67 +0.8 1,831,017 - Pf6N2E2_1568 0.67 +0.8
Or see this region's nucleotide sequence