Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_531 and Pf6N2E2_532 are separated by 484 nucleotides Pf6N2E2_532 and Pf6N2E2_533 are separated by 179 nucleotides Pf6N2E2_533 and Pf6N2E2_534 overlap by 4 nucleotides Pf6N2E2_534 and Pf6N2E2_535 overlap by 4 nucleotides Pf6N2E2_535 and Pf6N2E2_536 overlap by 4 nucleotides
Pf6N2E2_531 - FIG00966599: hypothetical protein, at 600,519 to 600,956
_531
Pf6N2E2_532 - Na(+) H(+) antiporter subunit G, at 601,441 to 601,554
_532
Pf6N2E2_533 - Na(+) H(+) antiporter subunit G, at 601,734 to 602,087
_533
Pf6N2E2_534 - Na(+) H(+) antiporter subunit F, at 602,084 to 602,362
_534
Pf6N2E2_535 - Na(+) H(+) antiporter subunit E, at 602,359 to 602,847
_535
Pf6N2E2_536 - Na(+) H(+) antiporter subunit D, at 602,844 to 604,529
_536
Position (kb) 601
602
603 Strain fitness (log2 ratio) -2
-1
0
1
2 at 601.169 kb on + strand at 601.979 kb on - strand, within Pf6N2E2_533 at 601.979 kb on - strand, within Pf6N2E2_533 at 601.979 kb on - strand, within Pf6N2E2_533 at 601.979 kb on - strand, within Pf6N2E2_533 at 601.979 kb on - strand, within Pf6N2E2_533 at 602.175 kb on + strand, within Pf6N2E2_534 at 602.176 kb on - strand, within Pf6N2E2_534 at 602.176 kb on - strand, within Pf6N2E2_534 at 602.176 kb on - strand, within Pf6N2E2_534 at 602.232 kb on + strand, within Pf6N2E2_534 at 602.232 kb on + strand, within Pf6N2E2_534 at 602.232 kb on + strand, within Pf6N2E2_534 at 602.232 kb on + strand, within Pf6N2E2_534 at 602.232 kb on + strand, within Pf6N2E2_534 at 602.232 kb on + strand, within Pf6N2E2_534 at 602.232 kb on + strand, within Pf6N2E2_534 at 602.233 kb on - strand, within Pf6N2E2_534 at 602.233 kb on - strand, within Pf6N2E2_534 at 602.233 kb on - strand, within Pf6N2E2_534 at 602.233 kb on - strand, within Pf6N2E2_534 at 602.233 kb on - strand, within Pf6N2E2_534 at 602.239 kb on - strand, within Pf6N2E2_534 at 602.239 kb on - strand, within Pf6N2E2_534 at 602.390 kb on + strand at 602.391 kb on - strand at 602.391 kb on - strand at 602.920 kb on + strand at 602.920 kb on + strand at 602.920 kb on + strand at 602.920 kb on + strand at 602.921 kb on - strand at 602.921 kb on - strand at 602.921 kb on - strand at 602.921 kb on - strand at 602.921 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 601,169 + -0.1 601,979 - Pf6N2E2_533 0.69 +0.9 601,979 - Pf6N2E2_533 0.69 -1.6 601,979 - Pf6N2E2_533 0.69 -0.1 601,979 - Pf6N2E2_533 0.69 -1.8 601,979 - Pf6N2E2_533 0.69 -0.8 602,175 + Pf6N2E2_534 0.33 +1.9 602,176 - Pf6N2E2_534 0.33 +0.9 602,176 - Pf6N2E2_534 0.33 +0.9 602,176 - Pf6N2E2_534 0.33 -0.4 602,232 + Pf6N2E2_534 0.53 -0.6 602,232 + Pf6N2E2_534 0.53 +0.9 602,232 + Pf6N2E2_534 0.53 -1.7 602,232 + Pf6N2E2_534 0.53 -0.1 602,232 + Pf6N2E2_534 0.53 +0.9 602,232 + Pf6N2E2_534 0.53 +0.9 602,232 + Pf6N2E2_534 0.53 -0.9 602,233 - Pf6N2E2_534 0.53 -0.7 602,233 - Pf6N2E2_534 0.53 -0.6 602,233 - Pf6N2E2_534 0.53 +0.9 602,233 - Pf6N2E2_534 0.53 -0.7 602,233 - Pf6N2E2_534 0.53 -0.7 602,239 - Pf6N2E2_534 0.56 +0.9 602,239 - Pf6N2E2_534 0.56 -0.1 602,390 + +0.9 602,391 - -0.1 602,391 - -0.5 602,920 + -1.6 602,920 + -0.1 602,920 + +0.9 602,920 + +0.9 602,921 - +0.9 602,921 - +0.3 602,921 - +0.9 602,921 - -0.7 602,921 - +0.3
Or see this region's nucleotide sequence