Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_485 and Pf6N2E2_486 overlap by 1 nucleotides Pf6N2E2_486 and Pf6N2E2_487 are separated by 75 nucleotides Pf6N2E2_487 and Pf6N2E2_488 are separated by 58 nucleotides
Pf6N2E2_485 - TRAP-type C4-dicarboxylate transport system, large permease component, at 551,539 to 552,819
_485
Pf6N2E2_486 - TRAP-type C4-dicarboxylate transport system, small permease component, at 552,819 to 553,346
_486
Pf6N2E2_487 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 553,422 to 554,390
_487
Pf6N2E2_488 - Gluconolactonase (EC 3.1.1.17), at 554,449 to 555,354
_488
Position (kb) 553
554
555 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 552.566 kb on - strand, within Pf6N2E2_485 at 552.787 kb on + strand at 552.946 kb on + strand, within Pf6N2E2_486 at 552.946 kb on + strand, within Pf6N2E2_486 at 552.946 kb on + strand, within Pf6N2E2_486 at 552.946 kb on + strand, within Pf6N2E2_486 at 552.946 kb on + strand, within Pf6N2E2_486 at 552.946 kb on + strand, within Pf6N2E2_486 at 552.947 kb on - strand, within Pf6N2E2_486 at 552.947 kb on - strand, within Pf6N2E2_486 at 552.947 kb on - strand, within Pf6N2E2_486 at 552.947 kb on - strand, within Pf6N2E2_486 at 553.056 kb on + strand, within Pf6N2E2_486 at 553.056 kb on + strand, within Pf6N2E2_486 at 553.056 kb on + strand, within Pf6N2E2_486 at 553.057 kb on - strand, within Pf6N2E2_486 at 553.057 kb on - strand, within Pf6N2E2_486 at 553.057 kb on - strand, within Pf6N2E2_486 at 553.057 kb on - strand, within Pf6N2E2_486 at 553.169 kb on + strand, within Pf6N2E2_486 at 553.169 kb on + strand, within Pf6N2E2_486 at 553.170 kb on - strand, within Pf6N2E2_486 at 553.173 kb on + strand, within Pf6N2E2_486 at 553.173 kb on + strand, within Pf6N2E2_486 at 553.173 kb on + strand, within Pf6N2E2_486 at 553.174 kb on - strand, within Pf6N2E2_486 at 553.309 kb on + strand at 553.309 kb on + strand at 553.325 kb on + strand at 553.325 kb on + strand at 553.326 kb on - strand at 553.359 kb on + strand at 553.359 kb on + strand at 553.424 kb on - strand at 553.440 kb on - strand at 553.501 kb on + strand at 553.501 kb on + strand at 553.501 kb on + strand at 553.502 kb on - strand at 553.502 kb on - strand at 553.502 kb on - strand at 553.717 kb on + strand, within Pf6N2E2_487 at 553.717 kb on + strand, within Pf6N2E2_487 at 553.717 kb on + strand, within Pf6N2E2_487 at 553.717 kb on + strand, within Pf6N2E2_487 at 553.718 kb on - strand, within Pf6N2E2_487 at 553.718 kb on - strand, within Pf6N2E2_487 at 553.718 kb on - strand, within Pf6N2E2_487 at 553.720 kb on + strand, within Pf6N2E2_487 at 553.720 kb on + strand, within Pf6N2E2_487 at 553.720 kb on + strand, within Pf6N2E2_487 at 553.720 kb on + strand, within Pf6N2E2_487 at 553.720 kb on + strand, within Pf6N2E2_487 at 553.721 kb on - strand, within Pf6N2E2_487 at 553.721 kb on - strand, within Pf6N2E2_487 at 553.735 kb on + strand, within Pf6N2E2_487 at 554.193 kb on + strand, within Pf6N2E2_487 at 554.194 kb on - strand, within Pf6N2E2_487 at 554.210 kb on - strand, within Pf6N2E2_487 at 554.375 kb on + strand at 554.375 kb on + strand at 554.376 kb on - strand at 554.376 kb on - strand at 554.376 kb on - strand at 554.711 kb on + strand, within Pf6N2E2_488 at 554.712 kb on - strand, within Pf6N2E2_488 at 554.814 kb on - strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.862 kb on - strand, within Pf6N2E2_488 at 554.960 kb on + strand, within Pf6N2E2_488 at 554.961 kb on - strand, within Pf6N2E2_488 at 554.961 kb on - strand, within Pf6N2E2_488 at 554.961 kb on - strand, within Pf6N2E2_488 at 555.231 kb on + strand, within Pf6N2E2_488 at 555.231 kb on + strand, within Pf6N2E2_488 at 555.276 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 552,566 - Pf6N2E2_485 0.80 -0.1 552,787 + +0.9 552,946 + Pf6N2E2_486 0.24 -0.1 552,946 + Pf6N2E2_486 0.24 +0.9 552,946 + Pf6N2E2_486 0.24 -1.3 552,946 + Pf6N2E2_486 0.24 -0.1 552,946 + Pf6N2E2_486 0.24 -0.7 552,946 + Pf6N2E2_486 0.24 +0.9 552,947 - Pf6N2E2_486 0.24 -0.1 552,947 - Pf6N2E2_486 0.24 +0.9 552,947 - Pf6N2E2_486 0.24 +0.9 552,947 - Pf6N2E2_486 0.24 +0.9 553,056 + Pf6N2E2_486 0.45 -1.4 553,056 + Pf6N2E2_486 0.45 -0.1 553,056 + Pf6N2E2_486 0.45 -0.7 553,057 - Pf6N2E2_486 0.45 -0.5 553,057 - Pf6N2E2_486 0.45 -0.7 553,057 - Pf6N2E2_486 0.45 -0.1 553,057 - Pf6N2E2_486 0.45 -0.5 553,169 + Pf6N2E2_486 0.66 +0.9 553,169 + Pf6N2E2_486 0.66 +0.9 553,170 - Pf6N2E2_486 0.66 +0.9 553,173 + Pf6N2E2_486 0.67 +0.9 553,173 + Pf6N2E2_486 0.67 -0.1 553,173 + Pf6N2E2_486 0.67 +0.5 553,174 - Pf6N2E2_486 0.67 +0.9 553,309 + -0.6 553,309 + -0.1 553,325 + -1.1 553,325 + +0.9 553,326 - +0.9 553,359 + +0.9 553,359 + +0.9 553,424 - -0.1 553,440 - -0.4 553,501 + +0.9 553,501 + +0.9 553,501 + +0.9 553,502 - -0.7 553,502 - -0.5 553,502 - +0.9 553,717 + Pf6N2E2_487 0.30 +0.9 553,717 + Pf6N2E2_487 0.30 -0.1 553,717 + Pf6N2E2_487 0.30 -3.4 553,717 + Pf6N2E2_487 0.30 +0.9 553,718 - Pf6N2E2_487 0.31 +0.9 553,718 - Pf6N2E2_487 0.31 -0.1 553,718 - Pf6N2E2_487 0.31 -1.2 553,720 + Pf6N2E2_487 0.31 +0.9 553,720 + Pf6N2E2_487 0.31 +0.9 553,720 + Pf6N2E2_487 0.31 +0.9 553,720 + Pf6N2E2_487 0.31 +0.9 553,720 + Pf6N2E2_487 0.31 -0.1 553,721 - Pf6N2E2_487 0.31 +0.9 553,721 - Pf6N2E2_487 0.31 -1.4 553,735 + Pf6N2E2_487 0.32 -1.0 554,193 + Pf6N2E2_487 0.80 +0.9 554,194 - Pf6N2E2_487 0.80 +0.9 554,210 - Pf6N2E2_487 0.81 -0.1 554,375 + +0.9 554,375 + -0.7 554,376 - +0.9 554,376 - -0.1 554,376 - +0.9 554,711 + Pf6N2E2_488 0.29 +0.9 554,712 - Pf6N2E2_488 0.29 +0.9 554,814 - Pf6N2E2_488 0.40 -1.7 554,861 + Pf6N2E2_488 0.45 +0.9 554,861 + Pf6N2E2_488 0.45 -0.3 554,861 + Pf6N2E2_488 0.45 -1.1 554,861 + Pf6N2E2_488 0.45 -0.7 554,861 + Pf6N2E2_488 0.45 +0.9 554,861 + Pf6N2E2_488 0.45 -0.6 554,862 - Pf6N2E2_488 0.46 +0.9 554,960 + Pf6N2E2_488 0.56 +0.9 554,961 - Pf6N2E2_488 0.57 +0.9 554,961 - Pf6N2E2_488 0.57 +0.9 554,961 - Pf6N2E2_488 0.57 -1.0 555,231 + Pf6N2E2_488 0.86 -1.1 555,231 + Pf6N2E2_488 0.86 +0.9 555,276 - -0.1
Or see this region's nucleotide sequence