Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3915 and Pf6N2E2_3916 are separated by 132 nucleotides Pf6N2E2_3916 and Pf6N2E2_3917 are separated by 12 nucleotides
Pf6N2E2_3915 - Transcriptional regulator, LysR family, at 4,550,214 to 4,551,104
_3915
Pf6N2E2_3916 - Short-chain dehydrogenase/reductase SDR, at 4,551,237 to 4,551,977
_3916
Pf6N2E2_3917 - High-affinity choline uptake protein BetT, at 4,551,990 to 4,553,993
_3917
Position (kb)
4551
4552 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4550.428 kb on + strand, within Pf6N2E2_3915 at 4550.469 kb on + strand, within Pf6N2E2_3915 at 4550.510 kb on - strand, within Pf6N2E2_3915 at 4550.536 kb on + strand, within Pf6N2E2_3915 at 4550.536 kb on + strand, within Pf6N2E2_3915 at 4550.536 kb on + strand, within Pf6N2E2_3915 at 4550.536 kb on + strand, within Pf6N2E2_3915 at 4550.536 kb on + strand, within Pf6N2E2_3915 at 4550.537 kb on - strand, within Pf6N2E2_3915 at 4550.537 kb on - strand, within Pf6N2E2_3915 at 4550.537 kb on - strand, within Pf6N2E2_3915 at 4550.537 kb on - strand, within Pf6N2E2_3915 at 4550.537 kb on - strand, within Pf6N2E2_3915 at 4550.537 kb on - strand, within Pf6N2E2_3915 at 4550.587 kb on + strand, within Pf6N2E2_3915 at 4550.672 kb on + strand, within Pf6N2E2_3915 at 4550.748 kb on + strand, within Pf6N2E2_3915 at 4550.748 kb on + strand, within Pf6N2E2_3915 at 4550.748 kb on + strand, within Pf6N2E2_3915 at 4550.748 kb on + strand, within Pf6N2E2_3915 at 4550.748 kb on + strand, within Pf6N2E2_3915 at 4550.749 kb on - strand, within Pf6N2E2_3915 at 4550.749 kb on - strand, within Pf6N2E2_3915 at 4550.749 kb on - strand, within Pf6N2E2_3915 at 4550.749 kb on - strand, within Pf6N2E2_3915 at 4550.749 kb on - strand, within Pf6N2E2_3915 at 4550.749 kb on - strand, within Pf6N2E2_3915 at 4550.798 kb on - strand, within Pf6N2E2_3915 at 4550.911 kb on + strand, within Pf6N2E2_3915 at 4550.911 kb on + strand, within Pf6N2E2_3915 at 4550.911 kb on + strand, within Pf6N2E2_3915 at 4550.911 kb on + strand, within Pf6N2E2_3915 at 4550.912 kb on - strand, within Pf6N2E2_3915 at 4551.023 kb on + strand at 4551.023 kb on + strand at 4551.023 kb on + strand at 4551.023 kb on + strand at 4551.024 kb on - strand at 4551.024 kb on - strand at 4551.024 kb on - strand at 4551.024 kb on - strand at 4551.064 kb on + strand at 4551.064 kb on + strand at 4551.064 kb on + strand at 4551.064 kb on + strand at 4551.064 kb on + strand at 4551.064 kb on + strand at 4551.065 kb on - strand at 4551.065 kb on - strand at 4551.065 kb on - strand at 4551.065 kb on - strand at 4551.065 kb on - strand at 4551.065 kb on - strand at 4551.065 kb on - strand at 4551.065 kb on - strand at 4551.067 kb on - strand at 4551.161 kb on + strand at 4551.165 kb on + strand at 4551.165 kb on + strand at 4551.177 kb on + strand at 4551.178 kb on - strand at 4551.345 kb on + strand, within Pf6N2E2_3916 at 4551.345 kb on + strand, within Pf6N2E2_3916 at 4551.345 kb on + strand, within Pf6N2E2_3916 at 4551.345 kb on + strand, within Pf6N2E2_3916 at 4551.696 kb on + strand, within Pf6N2E2_3916 at 4551.696 kb on + strand, within Pf6N2E2_3916 at 4551.696 kb on + strand, within Pf6N2E2_3916 at 4551.696 kb on + strand, within Pf6N2E2_3916 at 4551.697 kb on - strand, within Pf6N2E2_3916 at 4551.846 kb on + strand, within Pf6N2E2_3916 at 4551.847 kb on - strand, within Pf6N2E2_3916 at 4552.027 kb on + strand at 4552.027 kb on + strand at 4552.066 kb on + strand at 4552.066 kb on + strand at 4552.067 kb on - strand at 4552.081 kb on + strand at 4552.081 kb on + strand at 4552.120 kb on + strand at 4552.121 kb on - strand at 4552.124 kb on - strand at 4552.124 kb on - strand at 4552.124 kb on - strand at 4552.183 kb on + strand at 4552.204 kb on + strand, within Pf6N2E2_3917 at 4552.204 kb on + strand, within Pf6N2E2_3917 at 4552.205 kb on - strand, within Pf6N2E2_3917 at 4552.483 kb on + strand, within Pf6N2E2_3917 at 4552.483 kb on + strand, within Pf6N2E2_3917 at 4552.484 kb on - strand, within Pf6N2E2_3917 at 4552.484 kb on - strand, within Pf6N2E2_3917 at 4552.484 kb on - strand, within Pf6N2E2_3917 at 4552.778 kb on - strand, within Pf6N2E2_3917 at 4552.795 kb on + strand, within Pf6N2E2_3917 at 4552.795 kb on + strand, within Pf6N2E2_3917 at 4552.796 kb on - strand, within Pf6N2E2_3917 at 4552.796 kb on - strand, within Pf6N2E2_3917
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 4,550,428 + Pf6N2E2_3915 0.24 +1.1 4,550,469 + Pf6N2E2_3915 0.29 +1.1 4,550,510 - Pf6N2E2_3915 0.33 +0.1 4,550,536 + Pf6N2E2_3915 0.36 -0.7 4,550,536 + Pf6N2E2_3915 0.36 -0.5 4,550,536 + Pf6N2E2_3915 0.36 +1.1 4,550,536 + Pf6N2E2_3915 0.36 +1.1 4,550,536 + Pf6N2E2_3915 0.36 +0.1 4,550,537 - Pf6N2E2_3915 0.36 -0.7 4,550,537 - Pf6N2E2_3915 0.36 -0.1 4,550,537 - Pf6N2E2_3915 0.36 -0.5 4,550,537 - Pf6N2E2_3915 0.36 +0.2 4,550,537 - Pf6N2E2_3915 0.36 +2.1 4,550,537 - Pf6N2E2_3915 0.36 +0.1 4,550,587 + Pf6N2E2_3915 0.42 +1.1 4,550,672 + Pf6N2E2_3915 0.51 -0.9 4,550,748 + Pf6N2E2_3915 0.60 +1.1 4,550,748 + Pf6N2E2_3915 0.60 -0.5 4,550,748 + Pf6N2E2_3915 0.60 +1.1 4,550,748 + Pf6N2E2_3915 0.60 +1.1 4,550,748 + Pf6N2E2_3915 0.60 +0.1 4,550,749 - Pf6N2E2_3915 0.60 -0.5 4,550,749 - Pf6N2E2_3915 0.60 +0.1 4,550,749 - Pf6N2E2_3915 0.60 -1.3 4,550,749 - Pf6N2E2_3915 0.60 +0.8 4,550,749 - Pf6N2E2_3915 0.60 +1.1 4,550,749 - Pf6N2E2_3915 0.60 -0.9 4,550,798 - Pf6N2E2_3915 0.66 +1.1 4,550,911 + Pf6N2E2_3915 0.78 +0.1 4,550,911 + Pf6N2E2_3915 0.78 -0.3 4,550,911 + Pf6N2E2_3915 0.78 +1.1 4,550,911 + Pf6N2E2_3915 0.78 +1.1 4,550,912 - Pf6N2E2_3915 0.78 +1.1 4,551,023 + +0.6 4,551,023 + +1.1 4,551,023 + +1.1 4,551,023 + -0.7 4,551,024 - +0.1 4,551,024 - +1.1 4,551,024 - +0.1 4,551,024 - -0.9 4,551,064 + +0.2 4,551,064 + -0.5 4,551,064 + -0.9 4,551,064 + +1.1 4,551,064 + -0.2 4,551,064 + -0.5 4,551,065 - +1.1 4,551,065 - -1.7 4,551,065 - +0.1 4,551,065 - +1.1 4,551,065 - -0.5 4,551,065 - -0.6 4,551,065 - -0.9 4,551,065 - +0.1 4,551,067 - +1.1 4,551,161 + -1.4 4,551,165 + -0.9 4,551,165 + +1.1 4,551,177 + -0.5 4,551,178 - +1.1 4,551,345 + Pf6N2E2_3916 0.15 -1.6 4,551,345 + Pf6N2E2_3916 0.15 -0.5 4,551,345 + Pf6N2E2_3916 0.15 -1.3 4,551,345 + Pf6N2E2_3916 0.15 +1.1 4,551,696 + Pf6N2E2_3916 0.62 +1.1 4,551,696 + Pf6N2E2_3916 0.62 -0.9 4,551,696 + Pf6N2E2_3916 0.62 +0.1 4,551,696 + Pf6N2E2_3916 0.62 -1.8 4,551,697 - Pf6N2E2_3916 0.62 -0.9 4,551,846 + Pf6N2E2_3916 0.82 +1.1 4,551,847 - Pf6N2E2_3916 0.82 +0.1 4,552,027 + +0.3 4,552,027 + +0.1 4,552,066 + -1.0 4,552,066 + +1.1 4,552,067 - -0.5 4,552,081 + -0.9 4,552,081 + +1.1 4,552,120 + -0.7 4,552,121 - -1.1 4,552,124 - -0.5 4,552,124 - -0.5 4,552,124 - +1.1 4,552,183 + +1.1 4,552,204 + Pf6N2E2_3917 0.11 +0.4 4,552,204 + Pf6N2E2_3917 0.11 +1.1 4,552,205 - Pf6N2E2_3917 0.11 -0.3 4,552,483 + Pf6N2E2_3917 0.25 +0.1 4,552,483 + Pf6N2E2_3917 0.25 -1.3 4,552,484 - Pf6N2E2_3917 0.25 -0.5 4,552,484 - Pf6N2E2_3917 0.25 +0.1 4,552,484 - Pf6N2E2_3917 0.25 +1.1 4,552,778 - Pf6N2E2_3917 0.39 -1.3 4,552,795 + Pf6N2E2_3917 0.40 +1.1 4,552,795 + Pf6N2E2_3917 0.40 +1.1 4,552,796 - Pf6N2E2_3917 0.40 +0.1 4,552,796 - Pf6N2E2_3917 0.40 +1.1
Or see this region's nucleotide sequence