Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3677 and Pf6N2E2_3678 are separated by 84 nucleotides Pf6N2E2_3678 and Pf6N2E2_3679 are separated by 137 nucleotides
Pf6N2E2_3677 - Sensory box/GGDEF family protein, at 4,284,139 to 4,286,832
_3677
Pf6N2E2_3678 - Peptide methionine sulfoxide reductase MsrA (EC 1.8.4.11), at 4,286,917 to 4,287,564
_3678
Pf6N2E2_3679 - Protein involved in catabolism of external DNA, at 4,287,702 to 4,288,541
_3679
Position (kb)
4286
4287
4288 Strain fitness (log2 ratio)
-1
0
1
2 at 4286.047 kb on + strand, within Pf6N2E2_3677 at 4286.047 kb on + strand, within Pf6N2E2_3677 at 4286.047 kb on + strand, within Pf6N2E2_3677 at 4286.047 kb on + strand, within Pf6N2E2_3677 at 4286.048 kb on - strand, within Pf6N2E2_3677 at 4286.048 kb on - strand, within Pf6N2E2_3677 at 4286.110 kb on + strand, within Pf6N2E2_3677 at 4286.110 kb on + strand, within Pf6N2E2_3677 at 4286.111 kb on - strand, within Pf6N2E2_3677 at 4286.111 kb on - strand, within Pf6N2E2_3677 at 4286.111 kb on - strand, within Pf6N2E2_3677 at 4286.536 kb on + strand, within Pf6N2E2_3677 at 4286.552 kb on - strand, within Pf6N2E2_3677 at 4286.552 kb on - strand, within Pf6N2E2_3677 at 4286.841 kb on + strand at 4286.841 kb on + strand at 4286.841 kb on + strand at 4286.843 kb on + strand at 4286.843 kb on + strand at 4286.843 kb on + strand at 4286.844 kb on - strand at 4286.881 kb on - strand at 4286.881 kb on - strand at 4286.881 kb on - strand at 4287.181 kb on + strand, within Pf6N2E2_3678 at 4287.181 kb on + strand, within Pf6N2E2_3678 at 4287.182 kb on - strand, within Pf6N2E2_3678 at 4287.182 kb on - strand, within Pf6N2E2_3678 at 4287.235 kb on + strand, within Pf6N2E2_3678 at 4287.235 kb on + strand, within Pf6N2E2_3678 at 4287.257 kb on - strand, within Pf6N2E2_3678 at 4287.257 kb on - strand, within Pf6N2E2_3678 at 4287.352 kb on + strand, within Pf6N2E2_3678 at 4287.352 kb on + strand, within Pf6N2E2_3678 at 4287.352 kb on + strand, within Pf6N2E2_3678 at 4287.352 kb on + strand, within Pf6N2E2_3678 at 4287.352 kb on + strand, within Pf6N2E2_3678 at 4287.352 kb on + strand, within Pf6N2E2_3678 at 4287.353 kb on - strand, within Pf6N2E2_3678 at 4287.353 kb on - strand, within Pf6N2E2_3678 at 4287.353 kb on - strand, within Pf6N2E2_3678 at 4287.353 kb on - strand, within Pf6N2E2_3678 at 4287.472 kb on + strand, within Pf6N2E2_3678 at 4287.472 kb on + strand, within Pf6N2E2_3678 at 4287.473 kb on - strand, within Pf6N2E2_3678 at 4287.487 kb on + strand, within Pf6N2E2_3678 at 4287.488 kb on - strand, within Pf6N2E2_3678 at 4287.499 kb on + strand, within Pf6N2E2_3678 at 4287.523 kb on + strand at 4287.523 kb on + strand at 4287.523 kb on + strand at 4287.524 kb on - strand at 4287.635 kb on + strand at 4287.635 kb on + strand at 4287.641 kb on + strand at 4287.641 kb on + strand at 4287.641 kb on + strand at 4287.642 kb on - strand at 4287.642 kb on - strand at 4287.923 kb on - strand, within Pf6N2E2_3679 at 4288.256 kb on - strand, within Pf6N2E2_3679 at 4288.258 kb on + strand, within Pf6N2E2_3679 at 4288.258 kb on + strand, within Pf6N2E2_3679 at 4288.303 kb on + strand, within Pf6N2E2_3679 at 4288.303 kb on + strand, within Pf6N2E2_3679 at 4288.304 kb on - strand, within Pf6N2E2_3679 at 4288.304 kb on - strand, within Pf6N2E2_3679 at 4288.304 kb on - strand, within Pf6N2E2_3679 at 4288.364 kb on - strand, within Pf6N2E2_3679 at 4288.402 kb on + strand, within Pf6N2E2_3679 at 4288.403 kb on - strand, within Pf6N2E2_3679 at 4288.498 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 4,286,047 + Pf6N2E2_3677 0.71 +1.1 4,286,047 + Pf6N2E2_3677 0.71 +1.1 4,286,047 + Pf6N2E2_3677 0.71 -0.5 4,286,047 + Pf6N2E2_3677 0.71 +0.1 4,286,048 - Pf6N2E2_3677 0.71 +0.1 4,286,048 - Pf6N2E2_3677 0.71 -0.5 4,286,110 + Pf6N2E2_3677 0.73 +1.1 4,286,110 + Pf6N2E2_3677 0.73 +1.1 4,286,111 - Pf6N2E2_3677 0.73 +0.1 4,286,111 - Pf6N2E2_3677 0.73 -0.9 4,286,111 - Pf6N2E2_3677 0.73 -0.5 4,286,536 + Pf6N2E2_3677 0.89 +1.1 4,286,552 - Pf6N2E2_3677 0.90 +2.1 4,286,552 - Pf6N2E2_3677 0.90 -0.5 4,286,841 + -0.2 4,286,841 + +1.7 4,286,841 + -1.1 4,286,843 + -0.9 4,286,843 + -1.0 4,286,843 + +0.1 4,286,844 - +0.1 4,286,881 - -0.2 4,286,881 - +1.1 4,286,881 - -0.2 4,287,181 + Pf6N2E2_3678 0.41 -0.5 4,287,181 + Pf6N2E2_3678 0.41 +0.1 4,287,182 - Pf6N2E2_3678 0.41 +0.1 4,287,182 - Pf6N2E2_3678 0.41 -0.2 4,287,235 + Pf6N2E2_3678 0.49 +1.1 4,287,235 + Pf6N2E2_3678 0.49 -0.5 4,287,257 - Pf6N2E2_3678 0.52 +0.1 4,287,257 - Pf6N2E2_3678 0.52 +1.1 4,287,352 + Pf6N2E2_3678 0.67 +1.1 4,287,352 + Pf6N2E2_3678 0.67 -0.1 4,287,352 + Pf6N2E2_3678 0.67 +0.1 4,287,352 + Pf6N2E2_3678 0.67 +1.1 4,287,352 + Pf6N2E2_3678 0.67 +0.5 4,287,352 + Pf6N2E2_3678 0.67 +0.1 4,287,353 - Pf6N2E2_3678 0.67 -0.3 4,287,353 - Pf6N2E2_3678 0.67 -0.5 4,287,353 - Pf6N2E2_3678 0.67 -0.5 4,287,353 - Pf6N2E2_3678 0.67 +1.1 4,287,472 + Pf6N2E2_3678 0.86 +1.1 4,287,472 + Pf6N2E2_3678 0.86 +0.1 4,287,473 - Pf6N2E2_3678 0.86 +0.1 4,287,487 + Pf6N2E2_3678 0.88 +1.1 4,287,488 - Pf6N2E2_3678 0.88 +0.1 4,287,499 + Pf6N2E2_3678 0.90 -1.7 4,287,523 + -0.6 4,287,523 + +0.1 4,287,523 + +1.1 4,287,524 - +1.1 4,287,635 + -0.5 4,287,635 + +1.1 4,287,641 + +0.1 4,287,641 + +1.1 4,287,641 + +0.1 4,287,642 - +1.1 4,287,642 - +0.1 4,287,923 - Pf6N2E2_3679 0.26 -0.9 4,288,256 - Pf6N2E2_3679 0.66 +0.1 4,288,258 + Pf6N2E2_3679 0.66 +1.1 4,288,258 + Pf6N2E2_3679 0.66 +0.1 4,288,303 + Pf6N2E2_3679 0.72 -0.7 4,288,303 + Pf6N2E2_3679 0.72 -1.0 4,288,304 - Pf6N2E2_3679 0.72 +0.1 4,288,304 - Pf6N2E2_3679 0.72 -1.2 4,288,304 - Pf6N2E2_3679 0.72 -0.5 4,288,364 - Pf6N2E2_3679 0.79 +1.1 4,288,402 + Pf6N2E2_3679 0.83 +1.1 4,288,403 - Pf6N2E2_3679 0.83 -1.7 4,288,498 - +2.1
Or see this region's nucleotide sequence