Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2746 and Pf6N2E2_2747 are separated by 17 nucleotides Pf6N2E2_2747 and Pf6N2E2_2748 are separated by 315 nucleotides Pf6N2E2_2748 and Pf6N2E2_2749 are separated by 10 nucleotides
Pf6N2E2_2746 - Outer membrane protein NlpB, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and YfgL); Lipoprotein-34 precursor, at 3,238,554 to 3,239,669
_2746
Pf6N2E2_2747 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 3,239,687 to 3,240,565
_2747
Pf6N2E2_2748 - Glycine cleavage system transcriptional antiactivator GcvR, at 3,240,881 to 3,241,441
_2748
Pf6N2E2_2749 - Thiol peroxidase, Bcp-type (EC 1.11.1.15), at 3,241,452 to 3,241,925
_2749
Position (kb)
3239
3240
3241 Strain fitness (log2 ratio)
-2
-1
0
1 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3239.114 kb on - strand, within Pf6N2E2_2746 at 3239.114 kb on - strand, within Pf6N2E2_2746 at 3239.114 kb on - strand, within Pf6N2E2_2746 at 3239.114 kb on - strand, within Pf6N2E2_2746 at 3239.390 kb on - strand, within Pf6N2E2_2746 at 3239.441 kb on - strand, within Pf6N2E2_2746 at 3239.441 kb on - strand, within Pf6N2E2_2746 at 3239.585 kb on - strand at 3239.631 kb on - strand at 3239.631 kb on - strand at 3239.689 kb on - strand at 3240.633 kb on + strand at 3240.633 kb on + strand at 3240.634 kb on - strand at 3240.634 kb on - strand at 3240.696 kb on + strand at 3240.696 kb on + strand at 3240.696 kb on + strand at 3240.697 kb on - strand at 3240.697 kb on - strand at 3240.697 kb on - strand at 3240.953 kb on - strand, within Pf6N2E2_2748 at 3241.172 kb on + strand, within Pf6N2E2_2748 at 3241.172 kb on + strand, within Pf6N2E2_2748 at 3241.173 kb on - strand, within Pf6N2E2_2748 at 3241.181 kb on + strand, within Pf6N2E2_2748 at 3241.181 kb on + strand, within Pf6N2E2_2748 at 3241.181 kb on + strand, within Pf6N2E2_2748 at 3241.181 kb on + strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.439 kb on + strand at 3241.439 kb on + strand at 3241.439 kb on + strand at 3241.439 kb on + strand at 3241.439 kb on + strand at 3241.440 kb on - strand at 3241.440 kb on - strand at 3241.440 kb on - strand at 3241.440 kb on - strand at 3241.440 kb on - strand at 3241.554 kb on + strand, within Pf6N2E2_2749 at 3241.554 kb on + strand, within Pf6N2E2_2749 at 3241.554 kb on + strand, within Pf6N2E2_2749 at 3241.554 kb on + strand, within Pf6N2E2_2749 at 3241.554 kb on + strand, within Pf6N2E2_2749 at 3241.555 kb on - strand, within Pf6N2E2_2749 at 3241.555 kb on - strand, within Pf6N2E2_2749 at 3241.555 kb on - strand, within Pf6N2E2_2749 at 3241.560 kb on + strand, within Pf6N2E2_2749 at 3241.561 kb on - strand, within Pf6N2E2_2749 at 3241.561 kb on - strand, within Pf6N2E2_2749 at 3241.561 kb on - strand, within Pf6N2E2_2749
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 3,238,790 - Pf6N2E2_2746 0.21 -0.0 3,238,790 - Pf6N2E2_2746 0.21 +1.0 3,238,790 - Pf6N2E2_2746 0.21 +1.0 3,238,790 - Pf6N2E2_2746 0.21 +1.0 3,238,790 - Pf6N2E2_2746 0.21 -0.0 3,238,790 - Pf6N2E2_2746 0.21 +1.0 3,238,793 - Pf6N2E2_2746 0.21 +1.0 3,238,793 - Pf6N2E2_2746 0.21 -2.5 3,238,793 - Pf6N2E2_2746 0.21 -0.6 3,238,793 - Pf6N2E2_2746 0.21 +1.0 3,238,793 - Pf6N2E2_2746 0.21 +1.0 3,238,793 - Pf6N2E2_2746 0.21 +1.0 3,238,793 - Pf6N2E2_2746 0.21 +1.0 3,239,114 - Pf6N2E2_2746 0.50 +1.0 3,239,114 - Pf6N2E2_2746 0.50 +1.0 3,239,114 - Pf6N2E2_2746 0.50 -0.0 3,239,114 - Pf6N2E2_2746 0.50 -1.0 3,239,390 - Pf6N2E2_2746 0.75 -1.8 3,239,441 - Pf6N2E2_2746 0.79 +1.0 3,239,441 - Pf6N2E2_2746 0.79 +1.0 3,239,585 - +1.0 3,239,631 - +1.0 3,239,631 - +1.0 3,239,689 - -1.3 3,240,633 + -0.6 3,240,633 + +1.0 3,240,634 - +1.0 3,240,634 - +1.0 3,240,696 + -1.5 3,240,696 + +1.0 3,240,696 + -2.1 3,240,697 - +1.0 3,240,697 - -1.6 3,240,697 - -0.8 3,240,953 - Pf6N2E2_2748 0.13 -0.0 3,241,172 + Pf6N2E2_2748 0.52 -0.0 3,241,172 + Pf6N2E2_2748 0.52 +1.0 3,241,173 - Pf6N2E2_2748 0.52 -0.1 3,241,181 + Pf6N2E2_2748 0.53 +0.1 3,241,181 + Pf6N2E2_2748 0.53 +1.0 3,241,181 + Pf6N2E2_2748 0.53 +1.0 3,241,181 + Pf6N2E2_2748 0.53 +1.0 3,241,182 - Pf6N2E2_2748 0.54 -1.6 3,241,182 - Pf6N2E2_2748 0.54 +1.0 3,241,182 - Pf6N2E2_2748 0.54 +1.0 3,241,182 - Pf6N2E2_2748 0.54 -0.3 3,241,182 - Pf6N2E2_2748 0.54 -0.0 3,241,182 - Pf6N2E2_2748 0.54 -0.6 3,241,277 + Pf6N2E2_2748 0.71 -0.0 3,241,277 + Pf6N2E2_2748 0.71 -0.6 3,241,277 + Pf6N2E2_2748 0.71 -1.6 3,241,277 + Pf6N2E2_2748 0.71 -0.0 3,241,277 + Pf6N2E2_2748 0.71 -0.0 3,241,277 + Pf6N2E2_2748 0.71 +1.0 3,241,278 - Pf6N2E2_2748 0.71 -0.6 3,241,278 - Pf6N2E2_2748 0.71 -1.8 3,241,278 - Pf6N2E2_2748 0.71 +1.0 3,241,278 - Pf6N2E2_2748 0.71 -0.0 3,241,278 - Pf6N2E2_2748 0.71 +1.0 3,241,278 - Pf6N2E2_2748 0.71 -0.4 3,241,439 + -0.6 3,241,439 + -0.0 3,241,439 + +1.0 3,241,439 + -0.6 3,241,439 + +1.0 3,241,440 - -0.3 3,241,440 - -0.4 3,241,440 - +1.0 3,241,440 - -1.8 3,241,440 - -0.6 3,241,554 + Pf6N2E2_2749 0.22 -0.0 3,241,554 + Pf6N2E2_2749 0.22 -1.0 3,241,554 + Pf6N2E2_2749 0.22 -0.1 3,241,554 + Pf6N2E2_2749 0.22 -0.3 3,241,554 + Pf6N2E2_2749 0.22 -1.3 3,241,555 - Pf6N2E2_2749 0.22 -0.6 3,241,555 - Pf6N2E2_2749 0.22 -0.0 3,241,555 - Pf6N2E2_2749 0.22 -0.0 3,241,560 + Pf6N2E2_2749 0.23 -0.0 3,241,561 - Pf6N2E2_2749 0.23 +1.0 3,241,561 - Pf6N2E2_2749 0.23 +1.0 3,241,561 - Pf6N2E2_2749 0.23 -0.0
Or see this region's nucleotide sequence