Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2186 and Pf6N2E2_2187 are separated by 177 nucleotides Pf6N2E2_2187 and Pf6N2E2_2188 are separated by 197 nucleotides Pf6N2E2_2188 and Pf6N2E2_2189 are separated by 92 nucleotides
Pf6N2E2_2186 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4), at 2,590,235 to 2,591,464
_2186
Pf6N2E2_2187 - Transcriptional regulator, LysR family, at 2,591,642 to 2,592,571
_2187
Pf6N2E2_2188 - Predicted transcriptional regulator LiuR of leucine degradation pathway, MerR family, at 2,592,769 to 2,593,170
_2188
Pf6N2E2_2189 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 2,593,263 to 2,594,162
_2189
Position (kb)
2591
2592
2593 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2591.232 kb on + strand, within Pf6N2E2_2186 at 2591.398 kb on - strand at 2591.451 kb on + strand at 2591.495 kb on - strand at 2591.495 kb on - strand at 2591.495 kb on - strand at 2591.495 kb on - strand at 2591.495 kb on - strand at 2591.495 kb on - strand at 2591.495 kb on - strand at 2591.513 kb on + strand at 2591.513 kb on + strand at 2591.514 kb on - strand at 2591.537 kb on - strand at 2591.576 kb on - strand at 2591.627 kb on + strand at 2591.628 kb on - strand at 2591.693 kb on + strand at 2591.693 kb on + strand at 2591.693 kb on + strand at 2591.693 kb on + strand at 2591.694 kb on - strand at 2592.013 kb on + strand, within Pf6N2E2_2187 at 2592.013 kb on + strand, within Pf6N2E2_2187 at 2592.014 kb on - strand, within Pf6N2E2_2187 at 2592.014 kb on - strand, within Pf6N2E2_2187 at 2592.014 kb on - strand, within Pf6N2E2_2187 at 2592.123 kb on + strand, within Pf6N2E2_2187 at 2592.152 kb on + strand, within Pf6N2E2_2187 at 2592.152 kb on + strand, within Pf6N2E2_2187 at 2592.153 kb on - strand, within Pf6N2E2_2187 at 2592.153 kb on - strand, within Pf6N2E2_2187 at 2592.215 kb on + strand, within Pf6N2E2_2187 at 2592.238 kb on + strand, within Pf6N2E2_2187 at 2592.238 kb on + strand, within Pf6N2E2_2187 at 2592.239 kb on - strand, within Pf6N2E2_2187 at 2592.239 kb on - strand, within Pf6N2E2_2187 at 2592.266 kb on + strand, within Pf6N2E2_2187 at 2592.267 kb on - strand, within Pf6N2E2_2187 at 2592.267 kb on - strand, within Pf6N2E2_2187 at 2592.267 kb on - strand, within Pf6N2E2_2187 at 2592.329 kb on + strand, within Pf6N2E2_2187 at 2592.329 kb on + strand, within Pf6N2E2_2187 at 2592.329 kb on + strand, within Pf6N2E2_2187 at 2592.329 kb on + strand, within Pf6N2E2_2187 at 2592.329 kb on + strand, within Pf6N2E2_2187 at 2592.330 kb on - strand, within Pf6N2E2_2187 at 2592.330 kb on - strand, within Pf6N2E2_2187 at 2592.570 kb on - strand at 2592.631 kb on - strand at 2592.631 kb on - strand at 2592.643 kb on - strand at 2592.721 kb on - strand at 2592.732 kb on + strand at 2592.737 kb on + strand at 2592.784 kb on + strand at 2592.784 kb on + strand at 2592.785 kb on - strand at 2592.785 kb on - strand at 2592.785 kb on - strand at 2592.785 kb on - strand at 2592.841 kb on + strand, within Pf6N2E2_2188 at 2592.842 kb on - strand, within Pf6N2E2_2188 at 2592.842 kb on - strand, within Pf6N2E2_2188 at 2592.892 kb on + strand, within Pf6N2E2_2188 at 2592.986 kb on - strand, within Pf6N2E2_2188 at 2592.986 kb on - strand, within Pf6N2E2_2188 at 2592.986 kb on - strand, within Pf6N2E2_2188 at 2593.169 kb on - strand at 2593.234 kb on + strand at 2593.234 kb on + strand at 2593.234 kb on + strand at 2593.235 kb on - strand at 2593.235 kb on - strand at 2593.283 kb on - strand at 2593.283 kb on - strand at 2593.392 kb on + strand, within Pf6N2E2_2189 at 2593.486 kb on + strand, within Pf6N2E2_2189 at 2593.486 kb on + strand, within Pf6N2E2_2189 at 2593.491 kb on + strand, within Pf6N2E2_2189 at 2593.491 kb on + strand, within Pf6N2E2_2189 at 2593.492 kb on - strand, within Pf6N2E2_2189
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 2,591,232 + Pf6N2E2_2186 0.81 -0.6 2,591,398 - -0.1 2,591,451 + +0.9 2,591,495 - -0.8 2,591,495 - +0.9 2,591,495 - -1.4 2,591,495 - -0.2 2,591,495 - +0.9 2,591,495 - +0.9 2,591,495 - -0.1 2,591,513 + +0.9 2,591,513 + -0.1 2,591,514 - -0.6 2,591,537 - +0.9 2,591,576 - +0.9 2,591,627 + -0.1 2,591,628 - +0.9 2,591,693 + +0.9 2,591,693 + -2.2 2,591,693 + -2.8 2,591,693 + -0.3 2,591,694 - +0.9 2,592,013 + Pf6N2E2_2187 0.40 +0.9 2,592,013 + Pf6N2E2_2187 0.40 -0.5 2,592,014 - Pf6N2E2_2187 0.40 -1.4 2,592,014 - Pf6N2E2_2187 0.40 -1.1 2,592,014 - Pf6N2E2_2187 0.40 -1.4 2,592,123 + Pf6N2E2_2187 0.52 -0.6 2,592,152 + Pf6N2E2_2187 0.55 +0.9 2,592,152 + Pf6N2E2_2187 0.55 -1.0 2,592,153 - Pf6N2E2_2187 0.55 -1.6 2,592,153 - Pf6N2E2_2187 0.55 +0.9 2,592,215 + Pf6N2E2_2187 0.62 -1.1 2,592,238 + Pf6N2E2_2187 0.64 -2.9 2,592,238 + Pf6N2E2_2187 0.64 +0.9 2,592,239 - Pf6N2E2_2187 0.64 -1.4 2,592,239 - Pf6N2E2_2187 0.64 -0.1 2,592,266 + Pf6N2E2_2187 0.67 +0.9 2,592,267 - Pf6N2E2_2187 0.67 +0.9 2,592,267 - Pf6N2E2_2187 0.67 +0.4 2,592,267 - Pf6N2E2_2187 0.67 +0.9 2,592,329 + Pf6N2E2_2187 0.74 +0.4 2,592,329 + Pf6N2E2_2187 0.74 -1.6 2,592,329 + Pf6N2E2_2187 0.74 +0.9 2,592,329 + Pf6N2E2_2187 0.74 -0.1 2,592,329 + Pf6N2E2_2187 0.74 +0.9 2,592,330 - Pf6N2E2_2187 0.74 +0.9 2,592,330 - Pf6N2E2_2187 0.74 -0.5 2,592,570 - -0.6 2,592,631 - +0.9 2,592,631 - -0.1 2,592,643 - -0.1 2,592,721 - +0.9 2,592,732 + -0.1 2,592,737 + -0.1 2,592,784 + +0.9 2,592,784 + +1.3 2,592,785 - -0.6 2,592,785 - -0.1 2,592,785 - -0.6 2,592,785 - -0.1 2,592,841 + Pf6N2E2_2188 0.18 -2.5 2,592,842 - Pf6N2E2_2188 0.18 +0.9 2,592,842 - Pf6N2E2_2188 0.18 -0.6 2,592,892 + Pf6N2E2_2188 0.31 +0.9 2,592,986 - Pf6N2E2_2188 0.54 -2.1 2,592,986 - Pf6N2E2_2188 0.54 +0.9 2,592,986 - Pf6N2E2_2188 0.54 -0.1 2,593,169 - +0.9 2,593,234 + +0.9 2,593,234 + -0.1 2,593,234 + +0.9 2,593,235 - +0.9 2,593,235 - -0.2 2,593,283 - +0.9 2,593,283 - +0.9 2,593,392 + Pf6N2E2_2189 0.14 +0.9 2,593,486 + Pf6N2E2_2189 0.25 +0.9 2,593,486 + Pf6N2E2_2189 0.25 +0.9 2,593,491 + Pf6N2E2_2189 0.25 +0.9 2,593,491 + Pf6N2E2_2189 0.25 +0.9 2,593,492 - Pf6N2E2_2189 0.25 +0.9
Or see this region's nucleotide sequence