Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1802 and Pf6N2E2_1803 overlap by 8 nucleotides Pf6N2E2_1803 and Pf6N2E2_1804 overlap by 4 nucleotides Pf6N2E2_1804 and Pf6N2E2_1805 are separated by 49 nucleotides
Pf6N2E2_1802 - polysaccharide deacetylase, at 2,131,715 to 2,132,563
_1802
Pf6N2E2_1803 - Polysaccharide deacetylase, at 2,132,556 to 2,133,443
_1803
Pf6N2E2_1804 - Immune-responsive protein 1, at 2,133,440 to 2,134,834
_1804
Pf6N2E2_1805 - Multidrug resistance protein B, at 2,134,884 to 2,136,029
_1805
Position (kb)
2133
2134
2135 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2132.502 kb on + strand at 2133.114 kb on + strand, within Pf6N2E2_1803 at 2133.115 kb on - strand, within Pf6N2E2_1803 at 2133.172 kb on - strand, within Pf6N2E2_1803 at 2133.234 kb on + strand, within Pf6N2E2_1803 at 2133.235 kb on - strand, within Pf6N2E2_1803 at 2133.440 kb on - strand at 2133.541 kb on + strand at 2134.345 kb on - strand, within Pf6N2E2_1804 at 2134.424 kb on + strand, within Pf6N2E2_1804 at 2134.425 kb on - strand, within Pf6N2E2_1804 at 2134.620 kb on + strand, within Pf6N2E2_1804 at 2134.620 kb on + strand, within Pf6N2E2_1804 at 2134.620 kb on + strand, within Pf6N2E2_1804 at 2134.620 kb on + strand, within Pf6N2E2_1804 at 2134.621 kb on - strand, within Pf6N2E2_1804 at 2134.621 kb on - strand, within Pf6N2E2_1804 at 2134.723 kb on - strand at 2134.742 kb on - strand at 2134.742 kb on - strand at 2134.742 kb on - strand at 2134.842 kb on + strand at 2134.842 kb on + strand at 2134.843 kb on - strand at 2134.845 kb on - strand at 2134.956 kb on - strand at 2135.032 kb on - strand, within Pf6N2E2_1805 at 2135.032 kb on - strand, within Pf6N2E2_1805 at 2135.040 kb on + strand, within Pf6N2E2_1805 at 2135.535 kb on + strand, within Pf6N2E2_1805
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 2,132,502 + -1.4 2,133,114 + Pf6N2E2_1803 0.63 -0.1 2,133,115 - Pf6N2E2_1803 0.63 +0.9 2,133,172 - Pf6N2E2_1803 0.69 -0.1 2,133,234 + Pf6N2E2_1803 0.76 +0.9 2,133,235 - Pf6N2E2_1803 0.76 -0.4 2,133,440 - +0.9 2,133,541 + +0.9 2,134,345 - Pf6N2E2_1804 0.65 +0.9 2,134,424 + Pf6N2E2_1804 0.71 +0.9 2,134,425 - Pf6N2E2_1804 0.71 -0.1 2,134,620 + Pf6N2E2_1804 0.85 +0.9 2,134,620 + Pf6N2E2_1804 0.85 -0.1 2,134,620 + Pf6N2E2_1804 0.85 -3.4 2,134,620 + Pf6N2E2_1804 0.85 -2.1 2,134,621 - Pf6N2E2_1804 0.85 -0.7 2,134,621 - Pf6N2E2_1804 0.85 -1.6 2,134,723 - +0.9 2,134,742 - +0.9 2,134,742 - -0.6 2,134,742 - +0.9 2,134,842 + +0.9 2,134,842 + -0.8 2,134,843 - -0.1 2,134,845 - -0.1 2,134,956 - +0.9 2,135,032 - Pf6N2E2_1805 0.13 -0.1 2,135,032 - Pf6N2E2_1805 0.13 +0.9 2,135,040 + Pf6N2E2_1805 0.14 -0.1 2,135,535 + Pf6N2E2_1805 0.57 -1.0
Or see this region's nucleotide sequence