Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1788 and Pf6N2E2_1789 are separated by 30 nucleotides Pf6N2E2_1789 and Pf6N2E2_1790 are separated by 361 nucleotides
Pf6N2E2_1788 - Magnesium and cobalt transport protein CorA, at 2,114,995 to 2,115,945
_1788
Pf6N2E2_1789 - Kup system potassium uptake protein, at 2,115,976 to 2,117,817
_1789
Pf6N2E2_1790 - Sigma-54 dependent transcriptional regulator, at 2,118,179 to 2,120,170
_1790
Position (kb)
2115
2116
2117
2118 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2114.985 kb on + strand at 2114.985 kb on + strand at 2115.073 kb on - strand at 2115.234 kb on + strand, within Pf6N2E2_1788 at 2115.276 kb on + strand, within Pf6N2E2_1788 at 2115.277 kb on - strand, within Pf6N2E2_1788 at 2115.374 kb on + strand, within Pf6N2E2_1788 at 2115.374 kb on + strand, within Pf6N2E2_1788 at 2115.375 kb on - strand, within Pf6N2E2_1788 at 2115.375 kb on - strand, within Pf6N2E2_1788 at 2115.375 kb on - strand, within Pf6N2E2_1788 at 2115.375 kb on - strand, within Pf6N2E2_1788 at 2115.398 kb on - strand, within Pf6N2E2_1788 at 2115.514 kb on + strand, within Pf6N2E2_1788 at 2115.515 kb on - strand, within Pf6N2E2_1788 at 2115.517 kb on + strand, within Pf6N2E2_1788 at 2115.517 kb on + strand, within Pf6N2E2_1788 at 2115.518 kb on - strand, within Pf6N2E2_1788 at 2115.869 kb on + strand at 2115.870 kb on - strand at 2115.870 kb on - strand at 2115.929 kb on + strand at 2115.929 kb on + strand at 2115.929 kb on + strand at 2115.978 kb on - strand at 2116.003 kb on + strand at 2116.003 kb on + strand at 2116.147 kb on - strand at 2116.260 kb on - strand, within Pf6N2E2_1789 at 2116.260 kb on - strand, within Pf6N2E2_1789 at 2116.260 kb on - strand, within Pf6N2E2_1789 at 2116.327 kb on + strand, within Pf6N2E2_1789 at 2116.328 kb on - strand, within Pf6N2E2_1789 at 2116.431 kb on + strand, within Pf6N2E2_1789 at 2116.507 kb on - strand, within Pf6N2E2_1789 at 2116.507 kb on - strand, within Pf6N2E2_1789 at 2116.507 kb on - strand, within Pf6N2E2_1789 at 2116.745 kb on + strand, within Pf6N2E2_1789 at 2116.745 kb on + strand, within Pf6N2E2_1789 at 2116.746 kb on - strand, within Pf6N2E2_1789 at 2116.832 kb on + strand, within Pf6N2E2_1789 at 2116.832 kb on + strand, within Pf6N2E2_1789 at 2116.832 kb on + strand, within Pf6N2E2_1789 at 2116.832 kb on + strand, within Pf6N2E2_1789 at 2116.832 kb on + strand, within Pf6N2E2_1789 at 2116.832 kb on + strand, within Pf6N2E2_1789 at 2116.832 kb on + strand, within Pf6N2E2_1789 at 2116.832 kb on + strand, within Pf6N2E2_1789 at 2116.832 kb on + strand, within Pf6N2E2_1789 at 2116.833 kb on - strand, within Pf6N2E2_1789 at 2116.860 kb on + strand, within Pf6N2E2_1789 at 2116.860 kb on + strand, within Pf6N2E2_1789 at 2116.860 kb on + strand, within Pf6N2E2_1789 at 2116.860 kb on + strand, within Pf6N2E2_1789 at 2116.860 kb on + strand, within Pf6N2E2_1789 at 2116.886 kb on + strand, within Pf6N2E2_1789 at 2116.886 kb on + strand, within Pf6N2E2_1789 at 2117.009 kb on - strand, within Pf6N2E2_1789 at 2117.070 kb on - strand, within Pf6N2E2_1789 at 2117.150 kb on + strand, within Pf6N2E2_1789 at 2117.150 kb on + strand, within Pf6N2E2_1789 at 2117.151 kb on - strand, within Pf6N2E2_1789 at 2117.151 kb on - strand, within Pf6N2E2_1789 at 2117.151 kb on - strand, within Pf6N2E2_1789 at 2117.289 kb on - strand, within Pf6N2E2_1789 at 2117.289 kb on - strand, within Pf6N2E2_1789 at 2117.536 kb on + strand, within Pf6N2E2_1789 at 2117.649 kb on - strand at 2117.649 kb on - strand at 2117.649 kb on - strand at 2117.704 kb on + strand at 2117.704 kb on + strand at 2117.883 kb on - strand at 2117.910 kb on + strand at 2117.911 kb on - strand at 2117.911 kb on - strand at 2118.004 kb on + strand at 2118.011 kb on + strand at 2118.012 kb on - strand at 2118.035 kb on + strand at 2118.098 kb on + strand at 2118.120 kb on - strand at 2118.228 kb on + strand at 2118.246 kb on - strand at 2118.495 kb on + strand, within Pf6N2E2_1790 at 2118.495 kb on + strand, within Pf6N2E2_1790 at 2118.593 kb on + strand, within Pf6N2E2_1790 at 2118.593 kb on + strand, within Pf6N2E2_1790 at 2118.654 kb on + strand, within Pf6N2E2_1790 at 2118.655 kb on - strand, within Pf6N2E2_1790 at 2118.655 kb on - strand, within Pf6N2E2_1790 at 2118.788 kb on + strand, within Pf6N2E2_1790 at 2118.788 kb on + strand, within Pf6N2E2_1790 at 2118.789 kb on - strand, within Pf6N2E2_1790 at 2118.789 kb on - strand, within Pf6N2E2_1790 at 2118.789 kb on - strand, within Pf6N2E2_1790 at 2118.789 kb on - strand, within Pf6N2E2_1790 at 2118.789 kb on - strand, within Pf6N2E2_1790 at 2118.791 kb on + strand, within Pf6N2E2_1790 at 2118.791 kb on + strand, within Pf6N2E2_1790 at 2118.791 kb on + strand, within Pf6N2E2_1790 at 2118.791 kb on + strand, within Pf6N2E2_1790 at 2118.792 kb on - strand, within Pf6N2E2_1790 at 2118.792 kb on - strand, within Pf6N2E2_1790
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 2,114,985 + +0.9 2,114,985 + +0.9 2,115,073 - +0.9 2,115,234 + Pf6N2E2_1788 0.25 +0.9 2,115,276 + Pf6N2E2_1788 0.30 -0.1 2,115,277 - Pf6N2E2_1788 0.30 +0.9 2,115,374 + Pf6N2E2_1788 0.40 +0.9 2,115,374 + Pf6N2E2_1788 0.40 +0.9 2,115,375 - Pf6N2E2_1788 0.40 +0.9 2,115,375 - Pf6N2E2_1788 0.40 -0.1 2,115,375 - Pf6N2E2_1788 0.40 -0.7 2,115,375 - Pf6N2E2_1788 0.40 -2.9 2,115,398 - Pf6N2E2_1788 0.42 -0.1 2,115,514 + Pf6N2E2_1788 0.55 +0.3 2,115,515 - Pf6N2E2_1788 0.55 +0.3 2,115,517 + Pf6N2E2_1788 0.55 +0.9 2,115,517 + Pf6N2E2_1788 0.55 +0.9 2,115,518 - Pf6N2E2_1788 0.55 -0.1 2,115,869 + -0.6 2,115,870 - -0.1 2,115,870 - +0.9 2,115,929 + +0.9 2,115,929 + +0.9 2,115,929 + +0.9 2,115,978 - +0.9 2,116,003 + +0.9 2,116,003 + -0.1 2,116,147 - +0.9 2,116,260 - Pf6N2E2_1789 0.15 -0.7 2,116,260 - Pf6N2E2_1789 0.15 -0.1 2,116,260 - Pf6N2E2_1789 0.15 +0.9 2,116,327 + Pf6N2E2_1789 0.19 -0.7 2,116,328 - Pf6N2E2_1789 0.19 +0.3 2,116,431 + Pf6N2E2_1789 0.25 +0.9 2,116,507 - Pf6N2E2_1789 0.29 -0.1 2,116,507 - Pf6N2E2_1789 0.29 +0.9 2,116,507 - Pf6N2E2_1789 0.29 -1.3 2,116,745 + Pf6N2E2_1789 0.42 +0.9 2,116,745 + Pf6N2E2_1789 0.42 +0.9 2,116,746 - Pf6N2E2_1789 0.42 -0.9 2,116,832 + Pf6N2E2_1789 0.46 +0.9 2,116,832 + Pf6N2E2_1789 0.46 +0.9 2,116,832 + Pf6N2E2_1789 0.46 -1.0 2,116,832 + Pf6N2E2_1789 0.46 -0.3 2,116,832 + Pf6N2E2_1789 0.46 +0.9 2,116,832 + Pf6N2E2_1789 0.46 +0.9 2,116,832 + Pf6N2E2_1789 0.46 +0.9 2,116,832 + Pf6N2E2_1789 0.46 +0.9 2,116,832 + Pf6N2E2_1789 0.46 +0.9 2,116,833 - Pf6N2E2_1789 0.47 +0.9 2,116,860 + Pf6N2E2_1789 0.48 +0.9 2,116,860 + Pf6N2E2_1789 0.48 +0.9 2,116,860 + Pf6N2E2_1789 0.48 +0.9 2,116,860 + Pf6N2E2_1789 0.48 -0.1 2,116,860 + Pf6N2E2_1789 0.48 -0.1 2,116,886 + Pf6N2E2_1789 0.49 +0.9 2,116,886 + Pf6N2E2_1789 0.49 -1.7 2,117,009 - Pf6N2E2_1789 0.56 +0.9 2,117,070 - Pf6N2E2_1789 0.59 -0.0 2,117,150 + Pf6N2E2_1789 0.64 +0.9 2,117,150 + Pf6N2E2_1789 0.64 +0.9 2,117,151 - Pf6N2E2_1789 0.64 +0.9 2,117,151 - Pf6N2E2_1789 0.64 +0.8 2,117,151 - Pf6N2E2_1789 0.64 -0.1 2,117,289 - Pf6N2E2_1789 0.71 -0.1 2,117,289 - Pf6N2E2_1789 0.71 +0.9 2,117,536 + Pf6N2E2_1789 0.85 -0.1 2,117,649 - -0.1 2,117,649 - +0.9 2,117,649 - +0.2 2,117,704 + +0.9 2,117,704 + +0.9 2,117,883 - -0.7 2,117,910 + +0.9 2,117,911 - -0.1 2,117,911 - -0.1 2,118,004 + +0.9 2,118,011 + +0.9 2,118,012 - +0.9 2,118,035 + -0.1 2,118,098 + +0.9 2,118,120 - +0.3 2,118,228 + -0.1 2,118,246 - -0.7 2,118,495 + Pf6N2E2_1790 0.16 -0.7 2,118,495 + Pf6N2E2_1790 0.16 +0.9 2,118,593 + Pf6N2E2_1790 0.21 -0.7 2,118,593 + Pf6N2E2_1790 0.21 +0.9 2,118,654 + Pf6N2E2_1790 0.24 -0.7 2,118,655 - Pf6N2E2_1790 0.24 +0.9 2,118,655 - Pf6N2E2_1790 0.24 +0.9 2,118,788 + Pf6N2E2_1790 0.31 -0.7 2,118,788 + Pf6N2E2_1790 0.31 -0.1 2,118,789 - Pf6N2E2_1790 0.31 +1.2 2,118,789 - Pf6N2E2_1790 0.31 -1.4 2,118,789 - Pf6N2E2_1790 0.31 +0.9 2,118,789 - Pf6N2E2_1790 0.31 +0.9 2,118,789 - Pf6N2E2_1790 0.31 +0.9 2,118,791 + Pf6N2E2_1790 0.31 +0.9 2,118,791 + Pf6N2E2_1790 0.31 +0.9 2,118,791 + Pf6N2E2_1790 0.31 +0.1 2,118,791 + Pf6N2E2_1790 0.31 -2.7 2,118,792 - Pf6N2E2_1790 0.31 +0.9 2,118,792 - Pf6N2E2_1790 0.31 -1.0
Or see this region's nucleotide sequence