Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1380 and Pf6N2E2_1381 are separated by 458 nucleotides Pf6N2E2_1381 and Pf6N2E2_1382 are separated by 305 nucleotides
Pf6N2E2_1380 - Alcohol dehydrogenase (EC 1.1.1.1); Acetaldehyde dehydrogenase (EC 1.2.1.10), at 1,618,930 to 1,620,108
_1380
Pf6N2E2_1381 - Aldehyde dehydrogenase (EC 1.2.1.3), at 1,620,567 to 1,622,087
_1381
Pf6N2E2_1382 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 1,622,393 to 1,624,168
_1382
Position (kb)
1620
1621
1622
1623 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1620.097 kb on + strand at 1620.097 kb on + strand at 1620.098 kb on - strand at 1620.098 kb on - strand at 1620.113 kb on - strand at 1620.149 kb on - strand at 1620.236 kb on + strand at 1620.236 kb on + strand at 1620.236 kb on + strand at 1620.258 kb on + strand at 1620.259 kb on - strand at 1620.259 kb on - strand at 1620.259 kb on - strand at 1620.282 kb on + strand at 1620.334 kb on + strand at 1620.334 kb on + strand at 1620.365 kb on - strand at 1620.365 kb on - strand at 1620.365 kb on - strand at 1620.395 kb on + strand at 1620.396 kb on - strand at 1620.398 kb on + strand at 1620.398 kb on + strand at 1620.399 kb on - strand at 1620.493 kb on - strand at 1620.595 kb on + strand at 1620.596 kb on - strand at 1620.644 kb on + strand at 1620.644 kb on + strand at 1620.677 kb on - strand at 1620.937 kb on + strand, within Pf6N2E2_1381 at 1620.937 kb on + strand, within Pf6N2E2_1381 at 1620.937 kb on + strand, within Pf6N2E2_1381 at 1620.937 kb on + strand, within Pf6N2E2_1381 at 1620.937 kb on + strand, within Pf6N2E2_1381 at 1620.937 kb on + strand, within Pf6N2E2_1381 at 1620.937 kb on + strand at 1620.938 kb on - strand, within Pf6N2E2_1381 at 1620.938 kb on - strand, within Pf6N2E2_1381 at 1620.938 kb on - strand, within Pf6N2E2_1381 at 1620.938 kb on - strand, within Pf6N2E2_1381 at 1620.938 kb on - strand, within Pf6N2E2_1381 at 1620.938 kb on - strand, within Pf6N2E2_1381 at 1620.940 kb on + strand, within Pf6N2E2_1381 at 1620.941 kb on - strand, within Pf6N2E2_1381 at 1621.024 kb on + strand, within Pf6N2E2_1381 at 1621.025 kb on - strand, within Pf6N2E2_1381 at 1621.025 kb on - strand, within Pf6N2E2_1381 at 1621.604 kb on + strand, within Pf6N2E2_1381 at 1621.604 kb on + strand, within Pf6N2E2_1381 at 1621.708 kb on + strand, within Pf6N2E2_1381 at 1621.984 kb on + strand at 1621.984 kb on + strand at 1621.985 kb on - strand at 1622.080 kb on + strand at 1622.080 kb on + strand at 1622.141 kb on + strand at 1622.141 kb on + strand at 1622.142 kb on - strand at 1622.142 kb on - strand at 1622.192 kb on + strand at 1622.246 kb on - strand at 1622.529 kb on + strand at 1622.529 kb on + strand at 1622.529 kb on + strand at 1622.530 kb on - strand at 1622.704 kb on - strand, within Pf6N2E2_1382 at 1622.765 kb on - strand, within Pf6N2E2_1382 at 1622.765 kb on - strand, within Pf6N2E2_1382 at 1622.765 kb on - strand, within Pf6N2E2_1382 at 1622.765 kb on - strand, within Pf6N2E2_1382 at 1622.766 kb on + strand, within Pf6N2E2_1382 at 1622.766 kb on + strand, within Pf6N2E2_1382 at 1622.766 kb on + strand at 1622.766 kb on + strand, within Pf6N2E2_1382 at 1622.767 kb on - strand, within Pf6N2E2_1382 at 1622.767 kb on - strand, within Pf6N2E2_1382 at 1622.767 kb on - strand, within Pf6N2E2_1382 at 1622.767 kb on - strand, within Pf6N2E2_1382 at 1622.767 kb on - strand, within Pf6N2E2_1382 at 1622.826 kb on + strand, within Pf6N2E2_1382 at 1622.877 kb on + strand, within Pf6N2E2_1382 at 1622.877 kb on + strand, within Pf6N2E2_1382 at 1622.878 kb on - strand, within Pf6N2E2_1382 at 1622.878 kb on - strand, within Pf6N2E2_1382 at 1623.006 kb on + strand, within Pf6N2E2_1382 at 1623.006 kb on + strand, within Pf6N2E2_1382 at 1623.007 kb on - strand, within Pf6N2E2_1382
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 1,620,097 + +0.8 1,620,097 + -1.2 1,620,098 - -0.2 1,620,098 - +0.8 1,620,113 - +0.8 1,620,149 - +0.8 1,620,236 + -1.2 1,620,236 + +0.8 1,620,236 + +0.8 1,620,258 + -0.3 1,620,259 - +0.8 1,620,259 - -0.8 1,620,259 - +0.8 1,620,282 + +0.8 1,620,334 + +0.3 1,620,334 + -0.2 1,620,365 - -0.2 1,620,365 - +0.8 1,620,365 - -0.2 1,620,395 + -1.2 1,620,396 - -0.4 1,620,398 + -1.1 1,620,398 + +0.8 1,620,399 - +0.8 1,620,493 - -0.2 1,620,595 + +0.8 1,620,596 - -3.9 1,620,644 + +0.8 1,620,644 + +0.8 1,620,677 - -0.2 1,620,937 + Pf6N2E2_1381 0.24 +0.8 1,620,937 + Pf6N2E2_1381 0.24 -3.2 1,620,937 + Pf6N2E2_1381 0.24 -0.2 1,620,937 + Pf6N2E2_1381 0.24 +0.8 1,620,937 + Pf6N2E2_1381 0.24 -0.2 1,620,937 + Pf6N2E2_1381 0.24 +0.8 1,620,937 + +0.8 1,620,938 - Pf6N2E2_1381 0.24 -3.0 1,620,938 - Pf6N2E2_1381 0.24 -0.2 1,620,938 - Pf6N2E2_1381 0.24 -1.2 1,620,938 - Pf6N2E2_1381 0.24 +0.8 1,620,938 - Pf6N2E2_1381 0.24 +0.8 1,620,938 - Pf6N2E2_1381 0.24 -0.2 1,620,940 + Pf6N2E2_1381 0.25 -0.2 1,620,941 - Pf6N2E2_1381 0.25 -1.7 1,621,024 + Pf6N2E2_1381 0.30 -0.2 1,621,025 - Pf6N2E2_1381 0.30 -1.0 1,621,025 - Pf6N2E2_1381 0.30 +0.8 1,621,604 + Pf6N2E2_1381 0.68 -0.7 1,621,604 + Pf6N2E2_1381 0.68 +0.8 1,621,708 + Pf6N2E2_1381 0.75 +0.8 1,621,984 + -1.2 1,621,984 + -2.5 1,621,985 - +0.8 1,622,080 + +0.8 1,622,080 + -0.2 1,622,141 + +0.8 1,622,141 + -2.9 1,622,142 - +0.8 1,622,142 - -0.7 1,622,192 + -0.2 1,622,246 - -1.1 1,622,529 + +0.8 1,622,529 + +0.8 1,622,529 + -0.1 1,622,530 - -1.2 1,622,704 - Pf6N2E2_1382 0.18 +0.8 1,622,765 - Pf6N2E2_1382 0.21 +0.8 1,622,765 - Pf6N2E2_1382 0.21 +0.8 1,622,765 - Pf6N2E2_1382 0.21 -1.5 1,622,765 - Pf6N2E2_1382 0.21 -0.7 1,622,766 + Pf6N2E2_1382 0.21 -0.7 1,622,766 + Pf6N2E2_1382 0.21 +0.1 1,622,766 + +0.8 1,622,766 + Pf6N2E2_1382 0.21 -0.2 1,622,767 - Pf6N2E2_1382 0.21 +0.8 1,622,767 - Pf6N2E2_1382 0.21 +0.8 1,622,767 - Pf6N2E2_1382 0.21 +0.8 1,622,767 - Pf6N2E2_1382 0.21 +0.8 1,622,767 - Pf6N2E2_1382 0.21 +0.8 1,622,826 + Pf6N2E2_1382 0.24 +0.5 1,622,877 + Pf6N2E2_1382 0.27 +0.1 1,622,877 + Pf6N2E2_1382 0.27 +0.8 1,622,878 - Pf6N2E2_1382 0.27 -1.0 1,622,878 - Pf6N2E2_1382 0.27 -0.3 1,623,006 + Pf6N2E2_1382 0.35 +0.8 1,623,006 + Pf6N2E2_1382 0.35 +0.8 1,623,007 - Pf6N2E2_1382 0.35 +0.8
Or see this region's nucleotide sequence