Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3442

Experiment: Ying_OrganicAcid7 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3441 and Pf6N2E2_3442 are separated by 139 nucleotidesPf6N2E2_3442 and Pf6N2E2_3443 are separated by 33 nucleotides Pf6N2E2_3441 - Gluconate transporter family protein, at 4,010,266 to 4,011,615 _3441 Pf6N2E2_3442 - hypothetical protein, at 4,011,755 to 4,011,916 _3442 Pf6N2E2_3443 - Sulfate permease, at 4,011,950 to 4,013,395 _3443 Position (kb) 4011 4012Strain fitness (log2 ratio) -3 -2 -1 0 1at 4011.017 kb on + strand, within Pf6N2E2_3441at 4011.065 kb on + strand, within Pf6N2E2_3441at 4011.065 kb on + strand, within Pf6N2E2_3441at 4011.447 kb on - strand, within Pf6N2E2_3441at 4011.447 kb on - strand, within Pf6N2E2_3441at 4011.447 kb on - strand, within Pf6N2E2_3441at 4011.447 kb on - strand, within Pf6N2E2_3441at 4011.620 kb on + strandat 4011.621 kb on - strandat 4011.621 kb on - strandat 4011.661 kb on + strandat 4011.701 kb on - strandat 4011.944 kb on + strandat 4011.944 kb on + strandat 4011.945 kb on - strandat 4011.945 kb on - strandat 4012.038 kb on + strandat 4012.039 kb on - strandat 4012.039 kb on - strandat 4012.190 kb on + strand, within Pf6N2E2_3443at 4012.190 kb on + strand, within Pf6N2E2_3443at 4012.191 kb on - strand, within Pf6N2E2_3443at 4012.382 kb on + strand, within Pf6N2E2_3443at 4012.382 kb on + strand, within Pf6N2E2_3443at 4012.383 kb on - strand, within Pf6N2E2_3443at 4012.383 kb on - strand, within Pf6N2E2_3443at 4012.431 kb on + strand, within Pf6N2E2_3443at 4012.497 kb on - strand, within Pf6N2E2_3443

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 2
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4,011,017 + Pf6N2E2_3441 0.56 +1.0
4,011,065 + Pf6N2E2_3441 0.59 +1.0
4,011,065 + Pf6N2E2_3441 0.59 -1.1
4,011,447 - Pf6N2E2_3441 0.87 +0.0
4,011,447 - Pf6N2E2_3441 0.87 +0.0
4,011,447 - Pf6N2E2_3441 0.87 -0.9
4,011,447 - Pf6N2E2_3441 0.87 +1.0
4,011,620 + +0.0
4,011,621 - -1.5
4,011,621 - -2.9
4,011,661 + +0.0
4,011,701 - -0.6
4,011,944 + -0.6
4,011,944 + +1.0
4,011,945 - -0.4
4,011,945 - -0.2
4,012,038 + -1.0
4,012,039 - +1.0
4,012,039 - -0.9
4,012,190 + Pf6N2E2_3443 0.17 +0.0
4,012,190 + Pf6N2E2_3443 0.17 +0.0
4,012,191 - Pf6N2E2_3443 0.17 +0.6
4,012,382 + Pf6N2E2_3443 0.30 +1.0
4,012,382 + Pf6N2E2_3443 0.30 +0.4
4,012,383 - Pf6N2E2_3443 0.30 +1.0
4,012,383 - Pf6N2E2_3443 0.30 +0.0
4,012,431 + Pf6N2E2_3443 0.33 +0.0
4,012,497 - Pf6N2E2_3443 0.38 +1.0

Or see this region's nucleotide sequence