Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3169

Experiment: Ying_OrganicAcid7 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3168 and Pf6N2E2_3169 are separated by 3 nucleotidesPf6N2E2_3169 and Pf6N2E2_3170 are separated by 459 nucleotides Pf6N2E2_3168 - Exodeoxyribonuclease VII large subunit (EC 3.1.11.6), at 3,662,480 to 3,663,868 _3168 Pf6N2E2_3169 - Membrane proteins related to metalloendopeptidases, at 3,663,872 to 3,664,693 _3169 Pf6N2E2_3170 - 2-isopropylmalate synthase (EC 2.3.3.13), at 3,665,153 to 3,666,832 _3170 Position (kb) 3663 3664 3665Strain fitness (log2 ratio) -2 -1 0 1at 3663.256 kb on - strand, within Pf6N2E2_3168at 3663.503 kb on - strand, within Pf6N2E2_3168at 3663.576 kb on + strand, within Pf6N2E2_3168at 3663.714 kb on - strand, within Pf6N2E2_3168at 3663.817 kb on - strandat 3663.859 kb on - strandat 3663.891 kb on + strandat 3663.892 kb on - strandat 3663.935 kb on + strandat 3663.935 kb on + strandat 3663.935 kb on + strandat 3663.935 kb on + strandat 3663.935 kb on + strandat 3663.936 kb on - strandat 3663.936 kb on - strandat 3663.936 kb on - strandat 3663.936 kb on - strandat 3663.936 kb on - strandat 3664.022 kb on + strand, within Pf6N2E2_3169at 3664.163 kb on + strand, within Pf6N2E2_3169at 3664.164 kb on - strand, within Pf6N2E2_3169at 3664.460 kb on - strand, within Pf6N2E2_3169at 3664.460 kb on - strand, within Pf6N2E2_3169at 3664.463 kb on + strand, within Pf6N2E2_3169at 3664.463 kb on + strand, within Pf6N2E2_3169at 3664.463 kb on + strand, within Pf6N2E2_3169at 3664.463 kb on + strand, within Pf6N2E2_3169at 3664.463 kb on + strand, within Pf6N2E2_3169at 3664.464 kb on - strand, within Pf6N2E2_3169at 3664.464 kb on - strand, within Pf6N2E2_3169at 3664.464 kb on - strand, within Pf6N2E2_3169at 3664.464 kb on - strand, within Pf6N2E2_3169at 3664.464 kb on - strand, within Pf6N2E2_3169at 3664.748 kb on - strandat 3664.776 kb on - strandat 3665.184 kb on - strandat 3665.495 kb on - strand, within Pf6N2E2_3170at 3665.540 kb on + strand, within Pf6N2E2_3170at 3665.540 kb on + strand, within Pf6N2E2_3170at 3665.541 kb on - strand, within Pf6N2E2_3170at 3665.639 kb on + strand, within Pf6N2E2_3170at 3665.639 kb on + strand, within Pf6N2E2_3170at 3665.640 kb on - strand, within Pf6N2E2_3170at 3665.678 kb on + strand, within Pf6N2E2_3170

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 2
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3,663,256 - Pf6N2E2_3168 0.56 +1.1
3,663,503 - Pf6N2E2_3168 0.74 -0.6
3,663,576 + Pf6N2E2_3168 0.79 +1.1
3,663,714 - Pf6N2E2_3168 0.89 +0.1
3,663,817 - +0.1
3,663,859 - +0.1
3,663,891 + +0.1
3,663,892 - +0.5
3,663,935 + +0.1
3,663,935 + +0.1
3,663,935 + -2.1
3,663,935 + +0.1
3,663,935 + +1.1
3,663,936 - +0.1
3,663,936 - +0.1
3,663,936 - +1.1
3,663,936 - +1.1
3,663,936 - -0.4
3,664,022 + Pf6N2E2_3169 0.18 +1.1
3,664,163 + Pf6N2E2_3169 0.35 +1.1
3,664,164 - Pf6N2E2_3169 0.36 +0.1
3,664,460 - Pf6N2E2_3169 0.72 +0.1
3,664,460 - Pf6N2E2_3169 0.72 +1.1
3,664,463 + Pf6N2E2_3169 0.72 +0.1
3,664,463 + Pf6N2E2_3169 0.72 +0.1
3,664,463 + Pf6N2E2_3169 0.72 +1.1
3,664,463 + Pf6N2E2_3169 0.72 -0.5
3,664,463 + Pf6N2E2_3169 0.72 +1.1
3,664,464 - Pf6N2E2_3169 0.72 -0.5
3,664,464 - Pf6N2E2_3169 0.72 -0.5
3,664,464 - Pf6N2E2_3169 0.72 -0.9
3,664,464 - Pf6N2E2_3169 0.72 -0.5
3,664,464 - Pf6N2E2_3169 0.72 +1.1
3,664,748 - +0.1
3,664,776 - +1.1
3,665,184 - +0.1
3,665,495 - Pf6N2E2_3170 0.20 -1.3
3,665,540 + Pf6N2E2_3170 0.23 +1.1
3,665,540 + Pf6N2E2_3170 0.23 +1.1
3,665,541 - Pf6N2E2_3170 0.23 -0.9
3,665,639 + Pf6N2E2_3170 0.29 +1.1
3,665,639 + Pf6N2E2_3170 0.29 +1.1
3,665,640 - Pf6N2E2_3170 0.29 +0.1
3,665,678 + Pf6N2E2_3170 0.31 +1.1

Or see this region's nucleotide sequence