Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_638

Experiment: Ying_OrganicAcid7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_637 and Pf6N2E2_638 are separated by 195 nucleotidesPf6N2E2_638 and Pf6N2E2_639 are separated by 180 nucleotidesPf6N2E2_639 and Pf6N2E2_640 are separated by 205 nucleotides Pf6N2E2_637 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 728,851 to 730,284 _637 Pf6N2E2_638 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 730,480 to 730,920 _638 Pf6N2E2_639 - FIG01285020: hypothetical protein, at 731,101 to 731,442 _639 Pf6N2E2_640 - Outer membrane TonB-dependent transducer VreA of trans-envelope signaling system, at 731,648 to 732,280 _640 Position (kb) 730 731Strain fitness (log2 ratio) -1 0 1at 729.531 kb on - strand, within Pf6N2E2_637at 729.825 kb on - strand, within Pf6N2E2_637at 729.825 kb on - strand, within Pf6N2E2_637at 730.061 kb on + strand, within Pf6N2E2_637at 730.061 kb on + strand, within Pf6N2E2_637at 730.061 kb on + strand, within Pf6N2E2_637at 730.062 kb on - strand, within Pf6N2E2_637at 730.062 kb on - strand, within Pf6N2E2_637at 730.101 kb on - strand, within Pf6N2E2_637at 730.101 kb on - strand, within Pf6N2E2_637at 730.115 kb on + strand, within Pf6N2E2_637at 730.116 kb on - strand, within Pf6N2E2_637at 730.232 kb on + strandat 730.233 kb on - strandat 730.233 kb on - strandat 730.233 kb on - strandat 730.358 kb on + strandat 730.359 kb on - strandat 730.426 kb on + strandat 730.498 kb on + strandat 730.498 kb on + strandat 730.501 kb on + strandat 730.501 kb on + strandat 730.502 kb on - strandat 730.502 kb on - strandat 730.502 kb on - strandat 730.502 kb on - strandat 730.502 kb on - strandat 730.801 kb on + strand, within Pf6N2E2_638at 730.801 kb on + strand, within Pf6N2E2_638at 730.802 kb on - strand, within Pf6N2E2_638at 730.802 kb on - strand, within Pf6N2E2_638at 731.029 kb on - strandat 731.103 kb on - strandat 731.169 kb on + strand, within Pf6N2E2_639at 731.170 kb on - strand, within Pf6N2E2_639at 731.170 kb on - strand, within Pf6N2E2_639at 731.170 kb on - strand, within Pf6N2E2_639at 731.170 kb on - strand, within Pf6N2E2_639at 731.495 kb on + strandat 731.528 kb on + strandat 731.528 kb on + strandat 731.529 kb on - strandat 731.540 kb on - strandat 731.540 kb on - strandat 731.572 kb on + strandat 731.573 kb on - strandat 731.573 kb on - strandat 731.803 kb on + strand, within Pf6N2E2_640at 731.803 kb on + strand, within Pf6N2E2_640at 731.804 kb on - strand, within Pf6N2E2_640at 731.882 kb on + strand, within Pf6N2E2_640

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2
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729,531 - Pf6N2E2_637 0.47 -0.2
729,825 - Pf6N2E2_637 0.68 +0.4
729,825 - Pf6N2E2_637 0.68 +0.8
730,061 + Pf6N2E2_637 0.84 +0.8
730,061 + Pf6N2E2_637 0.84 -0.2
730,061 + Pf6N2E2_637 0.84 +0.1
730,062 - Pf6N2E2_637 0.84 -0.2
730,062 - Pf6N2E2_637 0.84 +0.8
730,101 - Pf6N2E2_637 0.87 +0.8
730,101 - Pf6N2E2_637 0.87 -0.2
730,115 + Pf6N2E2_637 0.88 -0.8
730,116 - Pf6N2E2_637 0.88 +0.8
730,232 + +0.8
730,233 - +0.8
730,233 - +0.8
730,233 - -0.3
730,358 + -1.5
730,359 - -0.2
730,426 + -0.2
730,498 + +0.8
730,498 + -0.2
730,501 + +0.1
730,501 + +0.8
730,502 - +0.8
730,502 - +0.8
730,502 - +0.3
730,502 - -0.2
730,502 - +0.8
730,801 + Pf6N2E2_638 0.73 -0.2
730,801 + Pf6N2E2_638 0.73 -0.2
730,802 - Pf6N2E2_638 0.73 -1.0
730,802 - Pf6N2E2_638 0.73 +0.8
731,029 - -0.8
731,103 - -0.5
731,169 + Pf6N2E2_639 0.20 +0.8
731,170 - Pf6N2E2_639 0.20 -0.5
731,170 - Pf6N2E2_639 0.20 +0.8
731,170 - Pf6N2E2_639 0.20 +0.8
731,170 - Pf6N2E2_639 0.20 -0.8
731,495 + +0.8
731,528 + +0.8
731,528 + +0.8
731,529 - +0.8
731,540 - -0.2
731,540 - +0.8
731,572 + -0.3
731,573 - -0.8
731,573 - -0.6
731,803 + Pf6N2E2_640 0.24 -0.2
731,803 + Pf6N2E2_640 0.24 +0.8
731,804 - Pf6N2E2_640 0.25 +0.4
731,882 + Pf6N2E2_640 0.37 +0.8

Or see this region's nucleotide sequence