Experiment: Ying_OrganicAcid7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_60 and Pf6N2E2_61 are separated by 69 nucleotides Pf6N2E2_61 and Pf6N2E2_62 overlap by 31 nucleotides Pf6N2E2_62 and Pf6N2E2_63 are separated by 157 nucleotides
Pf6N2E2_60 - Sensory box/GGDEF family protein, at 98,612 to 101,890
_60
Pf6N2E2_61 - Cys regulon transcriptional activator CysB, at 101,960 to 102,238
_61
Pf6N2E2_62 - Cys regulon transcriptional activator CysB, at 102,208 to 102,852
_62
Pf6N2E2_63 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 103,010 to 104,428
_63
Position (kb) 102
103 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 101.241 kb on - strand, within Pf6N2E2_60 at 101.331 kb on + strand, within Pf6N2E2_60 at 101.649 kb on - strand at 101.661 kb on + strand at 101.662 kb on - strand at 101.662 kb on - strand at 101.891 kb on + strand at 101.921 kb on + strand at 101.922 kb on - strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.962 kb on - strand at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.054 kb on + strand, within Pf6N2E2_61 at 102.054 kb on + strand, within Pf6N2E2_61 at 102.054 kb on + strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.059 kb on + strand, within Pf6N2E2_61 at 102.059 kb on + strand, within Pf6N2E2_61 at 102.060 kb on - strand, within Pf6N2E2_61 at 102.060 kb on - strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.078 kb on - strand, within Pf6N2E2_61 at 102.168 kb on - strand, within Pf6N2E2_61 at 102.180 kb on + strand, within Pf6N2E2_61 at 102.250 kb on + strand at 102.393 kb on - strand at 102.455 kb on + strand, within Pf6N2E2_62 at 102.550 kb on + strand, within Pf6N2E2_62 at 102.550 kb on + strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.823 kb on + strand at 102.824 kb on - strand at 102.824 kb on - strand at 102.824 kb on - strand at 102.824 kb on - strand at 102.825 kb on + strand at 102.825 kb on + strand at 102.825 kb on + strand at 102.825 kb on + strand at 102.826 kb on - strand at 102.826 kb on - strand at 102.920 kb on + strand at 102.921 kb on - strand at 102.929 kb on - strand at 102.955 kb on + strand at 102.981 kb on + strand at 102.982 kb on - strand at 103.028 kb on + strand at 103.028 kb on + strand at 103.088 kb on + strand at 103.088 kb on + strand at 103.088 kb on + strand at 103.206 kb on + strand, within Pf6N2E2_63 at 103.207 kb on - strand, within Pf6N2E2_63 at 103.288 kb on + strand, within Pf6N2E2_63 at 103.748 kb on + strand, within Pf6N2E2_63 at 103.749 kb on - strand, within Pf6N2E2_63 at 103.749 kb on - strand, within Pf6N2E2_63
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2 remove 101,241 - Pf6N2E2_60 0.80 -1.1 101,331 + Pf6N2E2_60 0.83 +0.9 101,649 - +0.9 101,661 + +0.9 101,662 - -1.4 101,662 - -0.1 101,891 + -0.8 101,921 + -0.1 101,922 - -0.5 101,961 + +0.9 101,961 + -0.1 101,961 + -0.7 101,961 + -0.0 101,961 + +0.9 101,962 - +0.9 102,052 + Pf6N2E2_61 0.33 +0.3 102,052 + Pf6N2E2_61 0.33 -0.5 102,052 + Pf6N2E2_61 0.33 -0.1 102,052 + Pf6N2E2_61 0.33 -0.7 102,052 + Pf6N2E2_61 0.33 +0.9 102,052 + Pf6N2E2_61 0.33 -1.0 102,052 + Pf6N2E2_61 0.33 -0.1 102,052 + Pf6N2E2_61 0.33 +0.9 102,053 - Pf6N2E2_61 0.33 -0.5 102,053 - Pf6N2E2_61 0.33 +0.9 102,053 - Pf6N2E2_61 0.33 +0.9 102,053 - Pf6N2E2_61 0.33 -0.7 102,053 - Pf6N2E2_61 0.33 +0.9 102,054 + Pf6N2E2_61 0.34 +0.9 102,054 + Pf6N2E2_61 0.34 +0.9 102,054 + Pf6N2E2_61 0.34 -0.1 102,055 - Pf6N2E2_61 0.34 -1.0 102,055 - Pf6N2E2_61 0.34 +0.9 102,055 - Pf6N2E2_61 0.34 -1.1 102,055 - Pf6N2E2_61 0.34 -0.1 102,059 + Pf6N2E2_61 0.35 -0.1 102,059 + Pf6N2E2_61 0.35 +0.6 102,060 - Pf6N2E2_61 0.36 +0.9 102,060 - Pf6N2E2_61 0.36 -0.7 102,077 + Pf6N2E2_61 0.42 +0.6 102,077 + Pf6N2E2_61 0.42 -0.2 102,077 + Pf6N2E2_61 0.42 -0.1 102,077 + Pf6N2E2_61 0.42 +0.9 102,078 - Pf6N2E2_61 0.42 +0.9 102,168 - Pf6N2E2_61 0.75 -0.4 102,180 + Pf6N2E2_61 0.79 -0.1 102,250 + +0.3 102,393 - -0.6 102,455 + Pf6N2E2_62 0.38 -0.1 102,550 + Pf6N2E2_62 0.53 -2.5 102,550 + Pf6N2E2_62 0.53 -1.1 102,551 - Pf6N2E2_62 0.53 +0.9 102,551 - Pf6N2E2_62 0.53 -0.4 102,551 - Pf6N2E2_62 0.53 -1.4 102,551 - Pf6N2E2_62 0.53 +0.9 102,823 + +0.9 102,824 - +0.9 102,824 - +0.9 102,824 - -3.9 102,824 - -0.1 102,825 + +0.9 102,825 + +0.9 102,825 + +0.9 102,825 + +0.9 102,826 - -4.0 102,826 - -5.3 102,920 + -2.1 102,921 - +0.9 102,929 - -7.4 102,955 + -0.7 102,981 + +0.9 102,982 - +0.9 103,028 + -0.7 103,028 + +0.9 103,088 + -1.4 103,088 + +0.9 103,088 + -0.7 103,206 + Pf6N2E2_63 0.14 +0.9 103,207 - Pf6N2E2_63 0.14 -7.4 103,288 + Pf6N2E2_63 0.20 -3.2 103,748 + Pf6N2E2_63 0.52 -0.1 103,749 - Pf6N2E2_63 0.52 -1.4 103,749 - Pf6N2E2_63 0.52 -0.1
Or see this region's nucleotide sequence