Experiment: Ying_OrganicAcid7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5374 and Pf6N2E2_5375 are separated by 272 nucleotides Pf6N2E2_5375 and Pf6N2E2_5376 are separated by 37 nucleotides Pf6N2E2_5376 and Pf6N2E2_5377 are separated by 171 nucleotides
Pf6N2E2_5374 - Membrane fusion component of tripartite multidrug resistance system, at 6,175,344 to 6,176,207
_5374
Pf6N2E2_5375 - Oxidoreductase, short chain dehydrogenase/reductase family, at 6,176,480 to 6,177,304
_5375
Pf6N2E2_5376 - Permeases of the drug/metabolite transporter (DMT) superfamily, at 6,177,342 to 6,177,806
_5376
Pf6N2E2_5377 - Mannose-6-phosphate isomerase (EC 5.3.1.8) / Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22), at 6,177,978 to 6,179,429
_5377
Position (kb)
6176
6177
6178 Strain fitness (log2 ratio)
-1
0
1
2 at 6175.821 kb on + strand, within Pf6N2E2_5374 at 6175.911 kb on + strand, within Pf6N2E2_5374 at 6175.911 kb on + strand, within Pf6N2E2_5374 at 6175.912 kb on - strand, within Pf6N2E2_5374 at 6175.912 kb on - strand, within Pf6N2E2_5374 at 6175.912 kb on - strand, within Pf6N2E2_5374 at 6176.028 kb on + strand, within Pf6N2E2_5374 at 6176.028 kb on + strand, within Pf6N2E2_5374 at 6176.029 kb on - strand, within Pf6N2E2_5374 at 6176.029 kb on - strand, within Pf6N2E2_5374 at 6176.137 kb on + strand at 6176.310 kb on + strand at 6176.364 kb on + strand at 6176.364 kb on + strand at 6176.364 kb on + strand at 6176.365 kb on - strand at 6176.365 kb on - strand at 6176.365 kb on - strand at 6176.457 kb on + strand at 6176.457 kb on + strand at 6176.458 kb on - strand at 6176.481 kb on + strand at 6176.482 kb on - strand at 6176.877 kb on + strand, within Pf6N2E2_5375 at 6177.192 kb on - strand, within Pf6N2E2_5375 at 6177.192 kb on - strand, within Pf6N2E2_5375 at 6177.529 kb on + strand, within Pf6N2E2_5376 at 6177.530 kb on - strand, within Pf6N2E2_5376 at 6177.530 kb on - strand, within Pf6N2E2_5376 at 6177.627 kb on - strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.628 kb on + strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.629 kb on - strand, within Pf6N2E2_5376 at 6177.633 kb on + strand, within Pf6N2E2_5376 at 6177.956 kb on + strand at 6177.956 kb on + strand at 6177.956 kb on + strand at 6177.956 kb on + strand at 6177.957 kb on - strand at 6177.957 kb on - strand at 6177.957 kb on - strand at 6177.957 kb on - strand at 6177.957 kb on - strand at 6177.980 kb on - strand at 6178.033 kb on + strand at 6178.033 kb on + strand at 6178.033 kb on + strand at 6178.034 kb on - strand at 6178.034 kb on - strand at 6178.034 kb on - strand at 6178.072 kb on + strand at 6178.072 kb on + strand at 6178.073 kb on - strand at 6178.153 kb on + strand, within Pf6N2E2_5377 at 6178.153 kb on + strand, within Pf6N2E2_5377 at 6178.153 kb on + strand, within Pf6N2E2_5377 at 6178.153 kb on + strand, within Pf6N2E2_5377 at 6178.153 kb on + strand, within Pf6N2E2_5377 at 6178.154 kb on - strand, within Pf6N2E2_5377 at 6178.154 kb on - strand, within Pf6N2E2_5377 at 6178.154 kb on - strand, within Pf6N2E2_5377
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2 remove 6,175,821 + Pf6N2E2_5374 0.55 +1.0 6,175,911 + Pf6N2E2_5374 0.66 +1.0 6,175,911 + Pf6N2E2_5374 0.66 -0.2 6,175,912 - Pf6N2E2_5374 0.66 +1.0 6,175,912 - Pf6N2E2_5374 0.66 +1.0 6,175,912 - Pf6N2E2_5374 0.66 +1.0 6,176,028 + Pf6N2E2_5374 0.79 -0.1 6,176,028 + Pf6N2E2_5374 0.79 +0.0 6,176,029 - Pf6N2E2_5374 0.79 -0.6 6,176,029 - Pf6N2E2_5374 0.79 -0.2 6,176,137 + -1.3 6,176,310 + -0.9 6,176,364 + +1.0 6,176,364 + +1.0 6,176,364 + +0.0 6,176,365 - +0.0 6,176,365 - +1.0 6,176,365 - +0.0 6,176,457 + +1.0 6,176,457 + +1.0 6,176,458 - +1.0 6,176,481 + +0.0 6,176,482 - +0.0 6,176,877 + Pf6N2E2_5375 0.48 -0.0 6,177,192 - Pf6N2E2_5375 0.86 +0.0 6,177,192 - Pf6N2E2_5375 0.86 +1.0 6,177,529 + Pf6N2E2_5376 0.40 +1.0 6,177,530 - Pf6N2E2_5376 0.40 -0.9 6,177,530 - Pf6N2E2_5376 0.40 +0.0 6,177,627 - Pf6N2E2_5376 0.61 +0.0 6,177,628 + Pf6N2E2_5376 0.62 -0.6 6,177,628 + Pf6N2E2_5376 0.62 +2.6 6,177,628 + Pf6N2E2_5376 0.62 -0.6 6,177,628 + Pf6N2E2_5376 0.62 -0.5 6,177,628 + Pf6N2E2_5376 0.62 +1.0 6,177,628 + Pf6N2E2_5376 0.62 +0.0 6,177,628 + Pf6N2E2_5376 0.62 +1.0 6,177,628 + Pf6N2E2_5376 0.62 +1.0 6,177,628 + Pf6N2E2_5376 0.62 +0.0 6,177,629 - Pf6N2E2_5376 0.62 -0.5 6,177,629 - Pf6N2E2_5376 0.62 +0.4 6,177,629 - Pf6N2E2_5376 0.62 +1.0 6,177,629 - Pf6N2E2_5376 0.62 +1.0 6,177,629 - Pf6N2E2_5376 0.62 -0.7 6,177,629 - Pf6N2E2_5376 0.62 +1.0 6,177,629 - Pf6N2E2_5376 0.62 +1.0 6,177,629 - Pf6N2E2_5376 0.62 -0.2 6,177,629 - Pf6N2E2_5376 0.62 -0.2 6,177,633 + Pf6N2E2_5376 0.63 +0.4 6,177,956 + +1.0 6,177,956 + +1.0 6,177,956 + +0.0 6,177,956 + +1.0 6,177,957 - -0.3 6,177,957 - -0.4 6,177,957 - -0.7 6,177,957 - +1.0 6,177,957 - +1.0 6,177,980 - +1.0 6,178,033 + -0.3 6,178,033 + +1.0 6,178,033 + +1.0 6,178,034 - -0.6 6,178,034 - -0.6 6,178,034 - +0.0 6,178,072 + -1.0 6,178,072 + +1.0 6,178,073 - +1.0 6,178,153 + Pf6N2E2_5377 0.12 -0.6 6,178,153 + Pf6N2E2_5377 0.12 +1.0 6,178,153 + Pf6N2E2_5377 0.12 -1.0 6,178,153 + Pf6N2E2_5377 0.12 +0.6 6,178,153 + Pf6N2E2_5377 0.12 +0.5 6,178,154 - Pf6N2E2_5377 0.12 +0.0 6,178,154 - Pf6N2E2_5377 0.12 -0.8 6,178,154 - Pf6N2E2_5377 0.12 +0.0
Or see this region's nucleotide sequence