Experiment: Ying_OrganicAcid7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4796 and Pf6N2E2_4797 overlap by 10 nucleotides Pf6N2E2_4797 and Pf6N2E2_4798 are separated by 79 nucleotides Pf6N2E2_4798 and Pf6N2E2_4799 are separated by 63 nucleotides
Pf6N2E2_4796 - Dihydroneopterin aldolase (EC 4.1.2.25), at 5,545,503 to 5,545,847
_4796
Pf6N2E2_4797 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 5,545,838 to 5,546,356
_4797
Pf6N2E2_4798 - tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25), at 5,546,436 to 5,547,662
_4798
Pf6N2E2_4799 - ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) , at 5,547,726 to 5,549,291
_4799
Position (kb)
5546
5547
5548 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5545.452 kb on + strand at 5545.452 kb on + strand at 5546.129 kb on + strand, within Pf6N2E2_4797 at 5546.130 kb on - strand, within Pf6N2E2_4797 at 5546.335 kb on + strand at 5546.335 kb on + strand at 5546.335 kb on + strand at 5546.335 kb on + strand at 5546.399 kb on - strand at 5546.399 kb on - strand at 5546.454 kb on - strand at 5546.455 kb on + strand at 5546.455 kb on + strand at 5546.455 kb on + strand at 5546.456 kb on - strand at 5546.456 kb on - strand at 5547.653 kb on - strand at 5547.653 kb on - strand at 5547.653 kb on - strand at 5547.653 kb on - strand at 5547.653 kb on - strand at 5547.653 kb on - strand at 5547.653 kb on - strand at 5547.663 kb on - strand at 5547.689 kb on + strand at 5547.761 kb on - strand at 5547.761 kb on - strand at 5547.761 kb on - strand at 5547.964 kb on + strand, within Pf6N2E2_4799 at 5547.964 kb on + strand, within Pf6N2E2_4799 at 5547.964 kb on + strand, within Pf6N2E2_4799 at 5547.964 kb on + strand, within Pf6N2E2_4799 at 5547.965 kb on - strand, within Pf6N2E2_4799 at 5547.965 kb on - strand, within Pf6N2E2_4799 at 5547.965 kb on - strand, within Pf6N2E2_4799 at 5547.965 kb on - strand, within Pf6N2E2_4799 at 5548.001 kb on - strand, within Pf6N2E2_4799 at 5548.001 kb on - strand, within Pf6N2E2_4799 at 5548.001 kb on - strand, within Pf6N2E2_4799 at 5548.001 kb on - strand, within Pf6N2E2_4799 at 5548.136 kb on - strand, within Pf6N2E2_4799 at 5548.136 kb on - strand, within Pf6N2E2_4799 at 5548.136 kb on - strand, within Pf6N2E2_4799 at 5548.136 kb on - strand, within Pf6N2E2_4799 at 5548.273 kb on + strand, within Pf6N2E2_4799 at 5548.273 kb on + strand, within Pf6N2E2_4799 at 5548.273 kb on + strand, within Pf6N2E2_4799 at 5548.273 kb on + strand, within Pf6N2E2_4799 at 5548.274 kb on - strand, within Pf6N2E2_4799 at 5548.274 kb on - strand, within Pf6N2E2_4799 at 5548.294 kb on + strand, within Pf6N2E2_4799 at 5548.295 kb on - strand, within Pf6N2E2_4799 at 5548.312 kb on + strand, within Pf6N2E2_4799 at 5548.312 kb on + strand, within Pf6N2E2_4799 at 5548.313 kb on - strand, within Pf6N2E2_4799 at 5548.313 kb on - strand, within Pf6N2E2_4799 at 5548.315 kb on + strand, within Pf6N2E2_4799 at 5548.315 kb on + strand, within Pf6N2E2_4799 at 5548.426 kb on + strand, within Pf6N2E2_4799 at 5548.427 kb on - strand, within Pf6N2E2_4799 at 5548.480 kb on + strand, within Pf6N2E2_4799 at 5548.481 kb on - strand, within Pf6N2E2_4799 at 5548.486 kb on + strand, within Pf6N2E2_4799 at 5548.486 kb on + strand, within Pf6N2E2_4799 at 5548.486 kb on + strand, within Pf6N2E2_4799 at 5548.486 kb on + strand, within Pf6N2E2_4799 at 5548.486 kb on + strand, within Pf6N2E2_4799 at 5548.486 kb on + strand, within Pf6N2E2_4799 at 5548.486 kb on + strand, within Pf6N2E2_4799 at 5548.487 kb on - strand, within Pf6N2E2_4799 at 5548.487 kb on - strand, within Pf6N2E2_4799 at 5548.487 kb on - strand, within Pf6N2E2_4799 at 5548.487 kb on - strand, within Pf6N2E2_4799 at 5548.564 kb on + strand, within Pf6N2E2_4799 at 5548.564 kb on + strand, within Pf6N2E2_4799 at 5548.564 kb on + strand, within Pf6N2E2_4799 at 5548.565 kb on - strand, within Pf6N2E2_4799 at 5548.618 kb on + strand, within Pf6N2E2_4799 at 5548.618 kb on + strand, within Pf6N2E2_4799 at 5548.618 kb on + strand, within Pf6N2E2_4799 at 5548.618 kb on + strand, within Pf6N2E2_4799 at 5548.618 kb on + strand, within Pf6N2E2_4799 at 5548.618 kb on + strand, within Pf6N2E2_4799 at 5548.619 kb on - strand, within Pf6N2E2_4799 at 5548.619 kb on - strand, within Pf6N2E2_4799 at 5548.619 kb on - strand, within Pf6N2E2_4799 at 5548.619 kb on - strand, within Pf6N2E2_4799
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2 remove 5,545,452 + -0.9 5,545,452 + -0.4 5,546,129 + Pf6N2E2_4797 0.56 -0.4 5,546,130 - Pf6N2E2_4797 0.56 +1.1 5,546,335 + -1.2 5,546,335 + +1.1 5,546,335 + +1.1 5,546,335 + +1.1 5,546,399 - +1.1 5,546,399 - -0.1 5,546,454 - +1.1 5,546,455 + -0.9 5,546,455 + +0.1 5,546,455 + -0.2 5,546,456 - -0.5 5,546,456 - +0.1 5,547,653 - +1.1 5,547,653 - +1.1 5,547,653 - -0.9 5,547,653 - +1.1 5,547,653 - +1.1 5,547,653 - +0.1 5,547,653 - +1.1 5,547,663 - +0.1 5,547,689 + +1.1 5,547,761 - +1.1 5,547,761 - -0.5 5,547,761 - +0.1 5,547,964 + Pf6N2E2_4799 0.15 +1.1 5,547,964 + Pf6N2E2_4799 0.15 +1.1 5,547,964 + Pf6N2E2_4799 0.15 +1.1 5,547,964 + Pf6N2E2_4799 0.15 -0.9 5,547,965 - Pf6N2E2_4799 0.15 +0.1 5,547,965 - Pf6N2E2_4799 0.15 -1.6 5,547,965 - Pf6N2E2_4799 0.15 +1.1 5,547,965 - Pf6N2E2_4799 0.15 +1.1 5,548,001 - Pf6N2E2_4799 0.18 +1.1 5,548,001 - Pf6N2E2_4799 0.18 -3.8 5,548,001 - Pf6N2E2_4799 0.18 +1.1 5,548,001 - Pf6N2E2_4799 0.18 +1.1 5,548,136 - Pf6N2E2_4799 0.26 -0.9 5,548,136 - Pf6N2E2_4799 0.26 -0.5 5,548,136 - Pf6N2E2_4799 0.26 +1.1 5,548,136 - Pf6N2E2_4799 0.26 -2.5 5,548,273 + Pf6N2E2_4799 0.35 +1.1 5,548,273 + Pf6N2E2_4799 0.35 -0.5 5,548,273 + Pf6N2E2_4799 0.35 -0.5 5,548,273 + Pf6N2E2_4799 0.35 -2.6 5,548,274 - Pf6N2E2_4799 0.35 -1.4 5,548,274 - Pf6N2E2_4799 0.35 -2.2 5,548,294 + Pf6N2E2_4799 0.36 -3.1 5,548,295 - Pf6N2E2_4799 0.36 +1.1 5,548,312 + Pf6N2E2_4799 0.37 -3.8 5,548,312 + Pf6N2E2_4799 0.37 -2.3 5,548,313 - Pf6N2E2_4799 0.37 +0.1 5,548,313 - Pf6N2E2_4799 0.37 +1.1 5,548,315 + Pf6N2E2_4799 0.38 -2.5 5,548,315 + Pf6N2E2_4799 0.38 +0.1 5,548,426 + Pf6N2E2_4799 0.45 +0.1 5,548,427 - Pf6N2E2_4799 0.45 -0.5 5,548,480 + Pf6N2E2_4799 0.48 -2.9 5,548,481 - Pf6N2E2_4799 0.48 -0.5 5,548,486 + Pf6N2E2_4799 0.49 +1.1 5,548,486 + Pf6N2E2_4799 0.49 +0.1 5,548,486 + Pf6N2E2_4799 0.49 +1.1 5,548,486 + Pf6N2E2_4799 0.49 +1.1 5,548,486 + Pf6N2E2_4799 0.49 -0.9 5,548,486 + Pf6N2E2_4799 0.49 +1.1 5,548,486 + Pf6N2E2_4799 0.49 -0.5 5,548,487 - Pf6N2E2_4799 0.49 -0.5 5,548,487 - Pf6N2E2_4799 0.49 -3.3 5,548,487 - Pf6N2E2_4799 0.49 +1.1 5,548,487 - Pf6N2E2_4799 0.49 -0.5 5,548,564 + Pf6N2E2_4799 0.54 -1.4 5,548,564 + Pf6N2E2_4799 0.54 -2.1 5,548,564 + Pf6N2E2_4799 0.54 -2.3 5,548,565 - Pf6N2E2_4799 0.54 -0.9 5,548,618 + Pf6N2E2_4799 0.57 +1.1 5,548,618 + Pf6N2E2_4799 0.57 +0.1 5,548,618 + Pf6N2E2_4799 0.57 +1.1 5,548,618 + Pf6N2E2_4799 0.57 +1.1 5,548,618 + Pf6N2E2_4799 0.57 +1.1 5,548,618 + Pf6N2E2_4799 0.57 +1.1 5,548,619 - Pf6N2E2_4799 0.57 +1.1 5,548,619 - Pf6N2E2_4799 0.57 -2.1 5,548,619 - Pf6N2E2_4799 0.57 -3.4 5,548,619 - Pf6N2E2_4799 0.57 +0.1
Or see this region's nucleotide sequence