Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4560

Experiment: Ying_OrganicAcid7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4559 and Pf6N2E2_4560 are separated by 4 nucleotidesPf6N2E2_4560 and Pf6N2E2_4561 are separated by 86 nucleotidesPf6N2E2_4561 and Pf6N2E2_4562 are separated by 144 nucleotides Pf6N2E2_4559 - probable integral membrane protein NMA1898, at 5,287,585 to 5,288,946 _4559 Pf6N2E2_4560 - probable integral membrane protein NMA1899, at 5,288,951 to 5,290,318 _4560 Pf6N2E2_4561 - Dihydrofolate reductase (EC 1.5.1.3), at 5,290,405 to 5,290,917 _4561 Pf6N2E2_4562 - L-cystine uptake protein TcyP, at 5,291,062 to 5,292,453 _4562 Position (kb) 5288 5289 5290 5291Strain fitness (log2 ratio) -1 0 1 2 3at 5288.372 kb on + strand, within Pf6N2E2_4559at 5288.372 kb on + strand, within Pf6N2E2_4559at 5288.373 kb on - strand, within Pf6N2E2_4559at 5288.552 kb on + strand, within Pf6N2E2_4559at 5288.552 kb on + strand, within Pf6N2E2_4559at 5288.553 kb on - strand, within Pf6N2E2_4559at 5288.553 kb on - strand, within Pf6N2E2_4559at 5288.553 kb on - strand, within Pf6N2E2_4559at 5288.588 kb on + strand, within Pf6N2E2_4559at 5288.588 kb on + strand, within Pf6N2E2_4559at 5288.588 kb on + strand, within Pf6N2E2_4559at 5288.588 kb on + strand, within Pf6N2E2_4559at 5288.589 kb on - strand, within Pf6N2E2_4559at 5288.774 kb on + strand, within Pf6N2E2_4559at 5288.774 kb on + strand, within Pf6N2E2_4559at 5288.774 kb on + strand, within Pf6N2E2_4559at 5288.775 kb on - strand, within Pf6N2E2_4559at 5288.775 kb on - strand, within Pf6N2E2_4559at 5288.775 kb on - strand, within Pf6N2E2_4559at 5288.883 kb on + strandat 5288.883 kb on + strandat 5289.435 kb on + strand, within Pf6N2E2_4560at 5289.531 kb on + strand, within Pf6N2E2_4560at 5289.531 kb on + strand, within Pf6N2E2_4560at 5289.532 kb on - strand, within Pf6N2E2_4560at 5289.532 kb on - strand, within Pf6N2E2_4560at 5289.706 kb on + strand, within Pf6N2E2_4560at 5289.755 kb on + strand, within Pf6N2E2_4560at 5289.755 kb on + strand, within Pf6N2E2_4560at 5290.202 kb on + strandat 5290.280 kb on + strandat 5290.310 kb on + strandat 5290.310 kb on + strandat 5290.353 kb on - strandat 5290.392 kb on + strandat 5290.926 kb on + strandat 5290.926 kb on + strandat 5290.926 kb on + strandat 5290.926 kb on + strandat 5290.926 kb on + strandat 5290.926 kb on + strandat 5290.927 kb on - strandat 5290.927 kb on - strandat 5290.927 kb on - strandat 5290.927 kb on - strandat 5290.927 kb on - strandat 5291.071 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2
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5,288,372 + Pf6N2E2_4559 0.58 +1.1
5,288,372 + Pf6N2E2_4559 0.58 -0.2
5,288,373 - Pf6N2E2_4559 0.58 +0.1
5,288,552 + Pf6N2E2_4559 0.71 +1.1
5,288,552 + Pf6N2E2_4559 0.71 +1.1
5,288,553 - Pf6N2E2_4559 0.71 -0.5
5,288,553 - Pf6N2E2_4559 0.71 +0.1
5,288,553 - Pf6N2E2_4559 0.71 +0.1
5,288,588 + Pf6N2E2_4559 0.74 +0.2
5,288,588 + Pf6N2E2_4559 0.74 +1.1
5,288,588 + Pf6N2E2_4559 0.74 -0.2
5,288,588 + Pf6N2E2_4559 0.74 +0.4
5,288,589 - Pf6N2E2_4559 0.74 -0.3
5,288,774 + Pf6N2E2_4559 0.87 +1.1
5,288,774 + Pf6N2E2_4559 0.87 +1.1
5,288,774 + Pf6N2E2_4559 0.87 +1.1
5,288,775 - Pf6N2E2_4559 0.87 +0.4
5,288,775 - Pf6N2E2_4559 0.87 -0.1
5,288,775 - Pf6N2E2_4559 0.87 -0.3
5,288,883 + +1.1
5,288,883 + +1.1
5,289,435 + Pf6N2E2_4560 0.35 +0.1
5,289,531 + Pf6N2E2_4560 0.42 +0.1
5,289,531 + Pf6N2E2_4560 0.42 +0.8
5,289,532 - Pf6N2E2_4560 0.42 +1.1
5,289,532 - Pf6N2E2_4560 0.42 +0.1
5,289,706 + Pf6N2E2_4560 0.55 +0.1
5,289,755 + Pf6N2E2_4560 0.59 +0.5
5,289,755 + Pf6N2E2_4560 0.59 +1.1
5,290,202 + +1.1
5,290,280 + -0.1
5,290,310 + +0.1
5,290,310 + +0.1
5,290,353 - -0.7
5,290,392 + +0.1
5,290,926 + +0.1
5,290,926 + +0.1
5,290,926 + -1.0
5,290,926 + +0.1
5,290,926 + -0.5
5,290,926 + +0.1
5,290,927 - -1.2
5,290,927 - -0.2
5,290,927 - +3.4
5,290,927 - +0.1
5,290,927 - +0.1
5,291,071 + +1.1

Or see this region's nucleotide sequence