Experiment: Ying_OrganicAcid7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3322 and Pf6N2E2_3323 are separated by 320 nucleotides Pf6N2E2_3323 and Pf6N2E2_3324 are separated by 53 nucleotides Pf6N2E2_3324 and Pf6N2E2_3325 are separated by 659 nucleotides
Pf6N2E2_3322 - Peptide synthetase, at 3,837,738 to 3,843,569
_3322
Pf6N2E2_3323 - hypothetical protein, at 3,843,890 to 3,844,618
_3323
Pf6N2E2_3324 - hypothetical protein, at 3,844,672 to 3,844,800
_3324
Pf6N2E2_3325 - ABC transporter, ATP-binding protein, at 3,845,460 to 3,846,485
_3325
Position (kb)
3843
3844
3845 Strain fitness (log2 ratio)
-2
-1
0
1 at 3843.090 kb on + strand at 3843.091 kb on - strand at 3843.102 kb on - strand at 3843.412 kb on + strand at 3843.412 kb on + strand at 3843.413 kb on - strand at 3843.413 kb on - strand at 3843.422 kb on + strand at 3843.436 kb on - strand at 3843.465 kb on - strand at 3843.465 kb on - strand at 3843.465 kb on - strand at 3843.538 kb on - strand at 3843.569 kb on + strand at 3843.570 kb on - strand at 3843.571 kb on + strand at 3843.571 kb on + strand at 3843.572 kb on - strand at 3843.572 kb on - strand at 3843.572 kb on - strand at 3843.597 kb on - strand at 3843.597 kb on - strand at 3843.597 kb on - strand at 3843.597 kb on - strand at 3843.614 kb on - strand at 3843.614 kb on - strand at 3843.614 kb on - strand at 3843.625 kb on - strand at 3843.690 kb on - strand at 3843.828 kb on + strand at 3843.829 kb on - strand at 3843.848 kb on + strand at 3843.862 kb on + strand at 3843.862 kb on + strand at 3843.862 kb on + strand at 3843.863 kb on - strand at 3843.869 kb on + strand at 3843.909 kb on + strand at 3844.049 kb on + strand, within Pf6N2E2_3323 at 3844.049 kb on + strand, within Pf6N2E2_3323 at 3844.052 kb on + strand, within Pf6N2E2_3323 at 3844.052 kb on + strand, within Pf6N2E2_3323 at 3844.318 kb on + strand, within Pf6N2E2_3323 at 3844.380 kb on + strand, within Pf6N2E2_3323 at 3844.380 kb on + strand, within Pf6N2E2_3323 at 3845.014 kb on + strand at 3845.014 kb on + strand at 3845.042 kb on + strand at 3845.238 kb on + strand at 3845.386 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2 remove 3,843,090 + +0.1 3,843,091 - +1.1 3,843,102 - +0.1 3,843,412 + -0.5 3,843,412 + +1.1 3,843,413 - +0.1 3,843,413 - +1.1 3,843,422 + -1.9 3,843,436 - -1.8 3,843,465 - +0.1 3,843,465 - -0.9 3,843,465 - +1.1 3,843,538 - +1.1 3,843,569 + -1.6 3,843,570 - +1.1 3,843,571 + +1.1 3,843,571 + +1.1 3,843,572 - +1.1 3,843,572 - +1.1 3,843,572 - +0.1 3,843,597 - +1.1 3,843,597 - +0.1 3,843,597 - +1.1 3,843,597 - +1.1 3,843,614 - -0.3 3,843,614 - -0.5 3,843,614 - +1.1 3,843,625 - +1.1 3,843,690 - -0.4 3,843,828 + -0.5 3,843,829 - +0.1 3,843,848 + +1.1 3,843,862 + +1.1 3,843,862 + -1.0 3,843,862 + -1.6 3,843,863 - +1.1 3,843,869 + -0.2 3,843,909 + +0.1 3,844,049 + Pf6N2E2_3323 0.22 -1.3 3,844,049 + Pf6N2E2_3323 0.22 -1.3 3,844,052 + Pf6N2E2_3323 0.22 +0.1 3,844,052 + Pf6N2E2_3323 0.22 +1.1 3,844,318 + Pf6N2E2_3323 0.59 +1.1 3,844,380 + Pf6N2E2_3323 0.67 -1.3 3,844,380 + Pf6N2E2_3323 0.67 +1.1 3,845,014 + +0.5 3,845,014 + +1.1 3,845,042 + +1.1 3,845,238 + +1.1 3,845,386 + -1.2
Or see this region's nucleotide sequence