Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3179

Experiment: Ying_OrganicAcid7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3178 and Pf6N2E2_3179 are separated by 3 nucleotidesPf6N2E2_3179 and Pf6N2E2_3180 overlap by 1 nucleotidesPf6N2E2_3180 and Pf6N2E2_3181 are separated by 12 nucleotides Pf6N2E2_3178 - 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (EC 1.17.7.1), at 3,674,452 to 3,675,561 _3178 Pf6N2E2_3179 - FIG021952: putative membrane protein, at 3,675,565 to 3,676,584 _3179 Pf6N2E2_3180 - Type IV pilus biogenesis protein PilF, at 3,676,584 to 3,677,342 _3180 Pf6N2E2_3181 - Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-), at 3,677,355 to 3,678,503 _3181 Position (kb) 3675 3676 3677Strain fitness (log2 ratio) -1 0 1at 3675.636 kb on - strandat 3675.636 kb on - strandat 3675.636 kb on - strandat 3675.660 kb on - strandat 3675.794 kb on - strand, within Pf6N2E2_3179at 3675.794 kb on - strand, within Pf6N2E2_3179at 3676.326 kb on - strand, within Pf6N2E2_3179at 3676.326 kb on - strand, within Pf6N2E2_3179at 3676.601 kb on - strandat 3676.601 kb on - strandat 3676.601 kb on - strandat 3676.601 kb on - strandat 3676.601 kb on - strandat 3676.601 kb on - strandat 3676.708 kb on - strand, within Pf6N2E2_3180at 3676.708 kb on - strand, within Pf6N2E2_3180at 3676.713 kb on - strand, within Pf6N2E2_3180at 3676.713 kb on - strand, within Pf6N2E2_3180at 3676.713 kb on - strand, within Pf6N2E2_3180at 3676.713 kb on - strand, within Pf6N2E2_3180at 3676.751 kb on - strand, within Pf6N2E2_3180at 3676.754 kb on - strand, within Pf6N2E2_3180at 3676.772 kb on - strand, within Pf6N2E2_3180at 3676.772 kb on - strand, within Pf6N2E2_3180at 3676.772 kb on - strand, within Pf6N2E2_3180at 3676.772 kb on - strand, within Pf6N2E2_3180at 3676.772 kb on - strand, within Pf6N2E2_3180at 3676.787 kb on - strand, within Pf6N2E2_3180at 3676.970 kb on - strand, within Pf6N2E2_3180at 3677.012 kb on - strand, within Pf6N2E2_3180at 3677.012 kb on - strand, within Pf6N2E2_3180at 3677.273 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.343 kb on - strandat 3677.357 kb on - strandat 3677.357 kb on - strandat 3677.420 kb on - strandat 3677.420 kb on - strandat 3677.420 kb on - strandat 3677.420 kb on - strandat 3677.420 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2
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3,675,636 - -0.6
3,675,636 - -0.6
3,675,636 - -1.6
3,675,660 - +1.0
3,675,794 - Pf6N2E2_3179 0.22 -1.0
3,675,794 - Pf6N2E2_3179 0.22 +1.0
3,676,326 - Pf6N2E2_3179 0.75 -1.3
3,676,326 - Pf6N2E2_3179 0.75 +1.0
3,676,601 - +0.0
3,676,601 - +0.0
3,676,601 - +0.0
3,676,601 - +0.0
3,676,601 - +1.0
3,676,601 - -0.6
3,676,708 - Pf6N2E2_3180 0.16 +1.0
3,676,708 - Pf6N2E2_3180 0.16 +1.0
3,676,713 - Pf6N2E2_3180 0.17 +0.0
3,676,713 - Pf6N2E2_3180 0.17 +1.0
3,676,713 - Pf6N2E2_3180 0.17 +0.0
3,676,713 - Pf6N2E2_3180 0.17 -0.5
3,676,751 - Pf6N2E2_3180 0.22 +0.0
3,676,754 - Pf6N2E2_3180 0.22 +1.0
3,676,772 - Pf6N2E2_3180 0.25 +0.3
3,676,772 - Pf6N2E2_3180 0.25 +0.0
3,676,772 - Pf6N2E2_3180 0.25 +0.0
3,676,772 - Pf6N2E2_3180 0.25 -0.6
3,676,772 - Pf6N2E2_3180 0.25 +1.0
3,676,787 - Pf6N2E2_3180 0.27 -0.6
3,676,970 - Pf6N2E2_3180 0.51 -0.6
3,677,012 - Pf6N2E2_3180 0.56 +0.0
3,677,012 - Pf6N2E2_3180 0.56 +1.0
3,677,273 - +1.0
3,677,343 - -0.6
3,677,343 - +1.0
3,677,343 - +0.0
3,677,343 - -0.4
3,677,343 - +0.0
3,677,343 - +0.0
3,677,343 - +1.0
3,677,343 - -0.2
3,677,343 - -0.6
3,677,343 - +0.0
3,677,343 - +0.1
3,677,343 - -1.3
3,677,343 - -1.0
3,677,343 - -0.6
3,677,343 - +0.0
3,677,343 - +0.0
3,677,343 - +0.0
3,677,357 - +1.0
3,677,357 - +0.0
3,677,420 - +1.0
3,677,420 - +0.0
3,677,420 - -0.2
3,677,420 - +0.0
3,677,420 - +1.0

Or see this region's nucleotide sequence