Experiment: Ying_OrganicAcid7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2498 and Pf6N2E2_2499 are separated by 154 nucleotides Pf6N2E2_2499 and Pf6N2E2_2500 are separated by 452 nucleotides Pf6N2E2_2500 and Pf6N2E2_2501 are separated by 155 nucleotides Pf6N2E2_2501 and Pf6N2E2_2502 are separated by 124 nucleotides
Pf6N2E2_2498 - Transcriptional regulator, GntR family, at 2,947,549 to 2,948,202
_2498
Pf6N2E2_2499 - Competence protein ComEA helix-hairpin-helix region precursor, at 2,948,357 to 2,948,692
_2499
Pf6N2E2_2500 - Putative membrane protein, at 2,949,145 to 2,950,236
_2500
Pf6N2E2_2501 - hypothetical protein, at 2,950,392 to 2,950,514
_2501
Pf6N2E2_2502 - O-antigen acetylase, at 2,950,639 to 2,952,537
_2502
Position (kb)
2949
2950
2951 Strain fitness (log2 ratio)
-1
0
1
2 at 2948.158 kb on + strand at 2948.193 kb on + strand at 2948.193 kb on + strand at 2948.193 kb on + strand at 2948.193 kb on + strand at 2948.193 kb on + strand at 2948.194 kb on - strand at 2948.194 kb on - strand at 2948.246 kb on + strand at 2948.247 kb on - strand at 2948.253 kb on + strand at 2948.253 kb on + strand at 2948.254 kb on - strand at 2948.254 kb on - strand at 2948.254 kb on - strand at 2948.258 kb on - strand at 2948.540 kb on + strand, within Pf6N2E2_2499 at 2948.554 kb on + strand, within Pf6N2E2_2499 at 2948.554 kb on + strand, within Pf6N2E2_2499 at 2948.554 kb on + strand, within Pf6N2E2_2499 at 2948.674 kb on - strand at 2948.679 kb on + strand at 2948.679 kb on + strand at 2948.680 kb on - strand at 2948.680 kb on - strand at 2948.692 kb on + strand at 2948.715 kb on + strand at 2948.716 kb on - strand at 2948.785 kb on + strand at 2948.786 kb on - strand at 2948.797 kb on + strand at 2948.801 kb on + strand at 2949.068 kb on - strand at 2949.128 kb on - strand at 2949.233 kb on - strand at 2949.246 kb on + strand at 2949.271 kb on - strand, within Pf6N2E2_2500 at 2949.292 kb on - strand, within Pf6N2E2_2500 at 2949.384 kb on - strand, within Pf6N2E2_2500 at 2949.397 kb on - strand, within Pf6N2E2_2500 at 2949.473 kb on + strand, within Pf6N2E2_2500 at 2949.501 kb on + strand, within Pf6N2E2_2500 at 2949.502 kb on - strand, within Pf6N2E2_2500 at 2949.541 kb on - strand, within Pf6N2E2_2500 at 2949.587 kb on - strand, within Pf6N2E2_2500 at 2950.076 kb on - strand, within Pf6N2E2_2500 at 2950.118 kb on + strand, within Pf6N2E2_2500 at 2950.192 kb on + strand at 2950.211 kb on - strand at 2950.259 kb on + strand at 2950.282 kb on + strand at 2950.310 kb on + strand at 2950.379 kb on - strand at 2950.457 kb on - strand, within Pf6N2E2_2501 at 2950.457 kb on - strand, within Pf6N2E2_2501 at 2950.459 kb on - strand, within Pf6N2E2_2501 at 2950.540 kb on - strand at 2950.618 kb on - strand at 2950.785 kb on - strand at 2951.169 kb on + strand, within Pf6N2E2_2502
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2 remove 2,948,158 + +0.9 2,948,193 + +0.9 2,948,193 + -0.6 2,948,193 + -0.7 2,948,193 + -0.7 2,948,193 + -0.1 2,948,194 - -0.1 2,948,194 - -0.1 2,948,246 + -0.1 2,948,247 - -0.7 2,948,253 + +0.9 2,948,253 + -0.1 2,948,254 - +0.9 2,948,254 - +0.9 2,948,254 - +0.9 2,948,258 - -0.1 2,948,540 + Pf6N2E2_2499 0.54 +0.9 2,948,554 + Pf6N2E2_2499 0.59 -0.1 2,948,554 + Pf6N2E2_2499 0.59 -0.1 2,948,554 + Pf6N2E2_2499 0.59 +0.9 2,948,674 - -0.1 2,948,679 + -0.1 2,948,679 + +0.9 2,948,680 - -0.5 2,948,680 - +0.5 2,948,692 + -1.1 2,948,715 + -0.1 2,948,716 - -0.7 2,948,785 + +0.9 2,948,786 - -0.6 2,948,797 + +0.9 2,948,801 + +0.9 2,949,068 - +0.9 2,949,128 - +0.9 2,949,233 - +0.9 2,949,246 + +0.9 2,949,271 - Pf6N2E2_2500 0.12 +0.3 2,949,292 - Pf6N2E2_2500 0.13 -1.4 2,949,384 - Pf6N2E2_2500 0.22 -0.3 2,949,397 - Pf6N2E2_2500 0.23 +0.9 2,949,473 + Pf6N2E2_2500 0.30 -0.1 2,949,501 + Pf6N2E2_2500 0.33 +0.9 2,949,502 - Pf6N2E2_2500 0.33 +1.9 2,949,541 - Pf6N2E2_2500 0.36 +0.3 2,949,587 - Pf6N2E2_2500 0.40 -0.1 2,950,076 - Pf6N2E2_2500 0.85 +0.9 2,950,118 + Pf6N2E2_2500 0.89 +0.7 2,950,192 + +1.5 2,950,211 - +0.9 2,950,259 + +0.9 2,950,282 + -0.3 2,950,310 + +0.9 2,950,379 - +0.9 2,950,457 - Pf6N2E2_2501 0.53 +0.9 2,950,457 - Pf6N2E2_2501 0.53 -0.7 2,950,459 - Pf6N2E2_2501 0.54 +1.3 2,950,540 - +0.9 2,950,618 - +0.9 2,950,785 - +0.9 2,951,169 + Pf6N2E2_2502 0.28 -0.1
Or see this region's nucleotide sequence