Experiment: Ying_OrganicAcid7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2487 and Pf6N2E2_2488 are separated by 105 nucleotides Pf6N2E2_2488 and Pf6N2E2_2489 are separated by 207 nucleotides Pf6N2E2_2489 and Pf6N2E2_2490 are separated by 178 nucleotides Pf6N2E2_2490 and Pf6N2E2_2491 are separated by 64 nucleotides
Pf6N2E2_2487 - FIG00956090: hypothetical protein, at 2,933,689 to 2,934,450
_2487
Pf6N2E2_2488 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 2,934,556 to 2,934,846
_2488
Pf6N2E2_2489 - UPF0028 protein YchK, at 2,935,054 to 2,936,091
_2489
Pf6N2E2_2490 - sensor histidine kinase, at 2,936,270 to 2,936,968
_2490
Pf6N2E2_2491 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 2,937,033 to 2,939,684
_2491
Position (kb)
2935
2936
2937 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2934.146 kb on + strand, within Pf6N2E2_2487 at 2934.457 kb on + strand at 2934.458 kb on - strand at 2934.527 kb on - strand at 2934.527 kb on - strand at 2934.527 kb on - strand at 2934.527 kb on - strand at 2934.584 kb on + strand at 2934.585 kb on - strand, within Pf6N2E2_2488 at 2934.680 kb on + strand, within Pf6N2E2_2488 at 2934.850 kb on + strand at 2934.851 kb on - strand at 2934.880 kb on + strand at 2934.880 kb on + strand at 2934.881 kb on - strand at 2934.881 kb on - strand at 2934.881 kb on - strand at 2934.883 kb on + strand at 2934.883 kb on + strand at 2934.884 kb on - strand at 2934.884 kb on - strand at 2934.884 kb on - strand at 2934.884 kb on - strand at 2934.925 kb on - strand at 2934.946 kb on + strand at 2934.946 kb on + strand at 2934.946 kb on + strand at 2934.946 kb on + strand at 2935.102 kb on + strand at 2935.145 kb on - strand at 2935.145 kb on - strand at 2935.198 kb on + strand, within Pf6N2E2_2489 at 2935.198 kb on + strand, within Pf6N2E2_2489 at 2935.198 kb on + strand, within Pf6N2E2_2489 at 2935.198 kb on + strand, within Pf6N2E2_2489 at 2935.198 kb on + strand, within Pf6N2E2_2489 at 2935.198 kb on + strand, within Pf6N2E2_2489 at 2935.199 kb on - strand, within Pf6N2E2_2489 at 2935.199 kb on - strand, within Pf6N2E2_2489 at 2935.250 kb on - strand, within Pf6N2E2_2489 at 2935.271 kb on + strand, within Pf6N2E2_2489 at 2935.372 kb on + strand, within Pf6N2E2_2489 at 2935.939 kb on + strand, within Pf6N2E2_2489 at 2935.939 kb on + strand, within Pf6N2E2_2489 at 2936.011 kb on + strand at 2936.011 kb on + strand at 2936.012 kb on - strand at 2936.058 kb on + strand at 2936.059 kb on - strand at 2936.272 kb on - strand at 2936.388 kb on + strand, within Pf6N2E2_2490 at 2936.490 kb on + strand, within Pf6N2E2_2490 at 2936.668 kb on - strand, within Pf6N2E2_2490 at 2936.668 kb on - strand, within Pf6N2E2_2490 at 2936.883 kb on + strand, within Pf6N2E2_2490 at 2936.883 kb on + strand, within Pf6N2E2_2490 at 2936.883 kb on + strand, within Pf6N2E2_2490 at 2936.883 kb on + strand, within Pf6N2E2_2490 at 2936.883 kb on + strand, within Pf6N2E2_2490 at 2936.884 kb on - strand, within Pf6N2E2_2490 at 2937.009 kb on - strand at 2937.067 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2 remove 2,934,146 + Pf6N2E2_2487 0.60 -1.4 2,934,457 + -0.1 2,934,458 - -0.1 2,934,527 - +0.8 2,934,527 - -0.5 2,934,527 - +0.9 2,934,527 - +0.9 2,934,584 + +0.9 2,934,585 - Pf6N2E2_2488 0.10 -0.6 2,934,680 + Pf6N2E2_2488 0.43 -0.1 2,934,850 + +0.9 2,934,851 - -0.1 2,934,880 + +1.9 2,934,880 + -0.1 2,934,881 - -0.3 2,934,881 - -2.1 2,934,881 - -0.4 2,934,883 + -0.1 2,934,883 + -0.1 2,934,884 - +0.9 2,934,884 - +0.9 2,934,884 - +0.6 2,934,884 - +0.9 2,934,925 - -0.1 2,934,946 + +0.9 2,934,946 + -0.9 2,934,946 + +0.9 2,934,946 + -0.3 2,935,102 + -1.1 2,935,145 - -0.1 2,935,145 - +0.4 2,935,198 + Pf6N2E2_2489 0.14 +0.9 2,935,198 + Pf6N2E2_2489 0.14 -0.1 2,935,198 + Pf6N2E2_2489 0.14 +0.9 2,935,198 + Pf6N2E2_2489 0.14 +0.9 2,935,198 + Pf6N2E2_2489 0.14 -0.1 2,935,198 + Pf6N2E2_2489 0.14 +0.9 2,935,199 - Pf6N2E2_2489 0.14 +0.3 2,935,199 - Pf6N2E2_2489 0.14 +0.9 2,935,250 - Pf6N2E2_2489 0.19 +0.9 2,935,271 + Pf6N2E2_2489 0.21 +0.9 2,935,372 + Pf6N2E2_2489 0.31 +1.9 2,935,939 + Pf6N2E2_2489 0.85 -1.1 2,935,939 + Pf6N2E2_2489 0.85 -0.1 2,936,011 + +0.5 2,936,011 + -0.2 2,936,012 - -1.1 2,936,058 + +0.4 2,936,059 - -1.1 2,936,272 - +0.9 2,936,388 + Pf6N2E2_2490 0.17 +0.9 2,936,490 + Pf6N2E2_2490 0.31 -0.5 2,936,668 - Pf6N2E2_2490 0.57 +0.9 2,936,668 - Pf6N2E2_2490 0.57 -0.1 2,936,883 + Pf6N2E2_2490 0.88 +0.9 2,936,883 + Pf6N2E2_2490 0.88 +0.9 2,936,883 + Pf6N2E2_2490 0.88 +0.9 2,936,883 + Pf6N2E2_2490 0.88 -0.6 2,936,883 + Pf6N2E2_2490 0.88 -2.4 2,936,884 - Pf6N2E2_2490 0.88 -0.5 2,937,009 - -0.2 2,937,067 - +0.9
Or see this region's nucleotide sequence