Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2365

Experiment: Ying_OrganicAcid7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2363 and Pf6N2E2_2364 are separated by 4 nucleotidesPf6N2E2_2364 and Pf6N2E2_2365 are separated by 8 nucleotidesPf6N2E2_2365 and Pf6N2E2_2366 overlap by 8 nucleotidesPf6N2E2_2366 and Pf6N2E2_2367 are separated by 541 nucleotides Pf6N2E2_2363 - Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components), at 2,798,350 to 2,799,153 _2363 Pf6N2E2_2364 - Type III secretion inner membrane protein (YscS,homologous to flagellar export components), at 2,799,158 to 2,799,412 _2364 Pf6N2E2_2365 - Type III secretion inner membrane protein (YscR,SpaR,HrcR,EscR,homologous to flagellar export components), at 2,799,421 to 2,800,080 _2365 Pf6N2E2_2366 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components), at 2,800,073 to 2,800,453 _2366 Pf6N2E2_2367 - hypothetical protein, at 2,800,995 to 2,801,870 _2367 Position (kb) 2799 2800 2801Strain fitness (log2 ratio) -1 0 1 2at 2798.465 kb on + strand, within Pf6N2E2_2363at 2798.659 kb on + strand, within Pf6N2E2_2363at 2798.693 kb on + strand, within Pf6N2E2_2363at 2798.745 kb on - strand, within Pf6N2E2_2363at 2798.908 kb on + strand, within Pf6N2E2_2363at 2798.985 kb on - strand, within Pf6N2E2_2363at 2799.125 kb on + strandat 2799.126 kb on - strandat 2799.159 kb on + strandat 2799.440 kb on + strandat 2799.599 kb on + strand, within Pf6N2E2_2365at 2799.599 kb on + strand, within Pf6N2E2_2365at 2799.599 kb on + strand, within Pf6N2E2_2365at 2799.605 kb on + strand, within Pf6N2E2_2365at 2799.605 kb on + strand, within Pf6N2E2_2365at 2799.605 kb on + strand, within Pf6N2E2_2365at 2799.606 kb on - strand, within Pf6N2E2_2365at 2799.768 kb on - strand, within Pf6N2E2_2365at 2799.779 kb on + strand, within Pf6N2E2_2365at 2799.788 kb on + strand, within Pf6N2E2_2365at 2799.789 kb on - strand, within Pf6N2E2_2365at 2799.789 kb on - strand, within Pf6N2E2_2365at 2799.838 kb on - strand, within Pf6N2E2_2365at 2799.860 kb on + strand, within Pf6N2E2_2365at 2799.860 kb on + strand, within Pf6N2E2_2365at 2799.860 kb on + strand, within Pf6N2E2_2365at 2799.860 kb on + strand, within Pf6N2E2_2365at 2799.860 kb on + strand, within Pf6N2E2_2365at 2799.861 kb on - strand, within Pf6N2E2_2365at 2799.861 kb on - strand, within Pf6N2E2_2365at 2799.861 kb on - strand, within Pf6N2E2_2365at 2799.864 kb on - strand, within Pf6N2E2_2365at 2799.864 kb on - strand, within Pf6N2E2_2365at 2800.004 kb on + strand, within Pf6N2E2_2365at 2800.005 kb on - strand, within Pf6N2E2_2365at 2800.370 kb on + strand, within Pf6N2E2_2366at 2800.371 kb on - strand, within Pf6N2E2_2366at 2800.371 kb on - strand, within Pf6N2E2_2366at 2800.371 kb on - strand, within Pf6N2E2_2366at 2800.371 kb on - strand, within Pf6N2E2_2366at 2800.384 kb on + strand, within Pf6N2E2_2366at 2800.385 kb on - strand, within Pf6N2E2_2366at 2800.878 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2
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2,798,465 + Pf6N2E2_2363 0.14 +1.0
2,798,659 + Pf6N2E2_2363 0.38 +0.7
2,798,693 + Pf6N2E2_2363 0.43 +1.0
2,798,745 - Pf6N2E2_2363 0.49 -0.6
2,798,908 + Pf6N2E2_2363 0.69 -0.6
2,798,985 - Pf6N2E2_2363 0.79 +1.0
2,799,125 + -0.0
2,799,126 - +1.0
2,799,159 + +1.0
2,799,440 + -0.0
2,799,599 + Pf6N2E2_2365 0.27 -0.1
2,799,599 + Pf6N2E2_2365 0.27 +0.6
2,799,599 + Pf6N2E2_2365 0.27 -0.6
2,799,605 + Pf6N2E2_2365 0.28 -0.0
2,799,605 + Pf6N2E2_2365 0.28 -0.0
2,799,605 + Pf6N2E2_2365 0.28 -0.0
2,799,606 - Pf6N2E2_2365 0.28 +1.3
2,799,768 - Pf6N2E2_2365 0.53 -0.0
2,799,779 + Pf6N2E2_2365 0.54 -0.3
2,799,788 + Pf6N2E2_2365 0.56 +0.6
2,799,789 - Pf6N2E2_2365 0.56 -1.0
2,799,789 - Pf6N2E2_2365 0.56 -0.0
2,799,838 - Pf6N2E2_2365 0.63 +1.0
2,799,860 + Pf6N2E2_2365 0.67 -0.0
2,799,860 + Pf6N2E2_2365 0.67 -0.3
2,799,860 + Pf6N2E2_2365 0.67 +1.0
2,799,860 + Pf6N2E2_2365 0.67 -0.2
2,799,860 + Pf6N2E2_2365 0.67 +1.0
2,799,861 - Pf6N2E2_2365 0.67 -1.1
2,799,861 - Pf6N2E2_2365 0.67 -0.1
2,799,861 - Pf6N2E2_2365 0.67 +2.0
2,799,864 - Pf6N2E2_2365 0.67 -0.6
2,799,864 - Pf6N2E2_2365 0.67 -0.0
2,800,004 + Pf6N2E2_2365 0.88 -0.0
2,800,005 - Pf6N2E2_2365 0.88 -0.6
2,800,370 + Pf6N2E2_2366 0.78 -0.2
2,800,371 - Pf6N2E2_2366 0.78 +0.3
2,800,371 - Pf6N2E2_2366 0.78 +1.0
2,800,371 - Pf6N2E2_2366 0.78 +1.0
2,800,371 - Pf6N2E2_2366 0.78 -0.4
2,800,384 + Pf6N2E2_2366 0.82 -0.0
2,800,385 - Pf6N2E2_2366 0.82 -0.6
2,800,878 - -0.0

Or see this region's nucleotide sequence