Experiment: Ying_OrganicAcid7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2097 and Pf6N2E2_2098 overlap by 4 nucleotides Pf6N2E2_2098 and Pf6N2E2_2099 are separated by 114 nucleotides Pf6N2E2_2099 and Pf6N2E2_2100 are separated by 242 nucleotides
Pf6N2E2_2097 - Fe2+-dicitrate sensor, membrane component, at 2,496,012 to 2,496,971
_2097
Pf6N2E2_2098 - heme uptake regulator, at 2,496,968 to 2,497,489
_2098
Pf6N2E2_2099 - response regulator receiver protein, at 2,497,604 to 2,498,011
_2099
Pf6N2E2_2100 - DNA topoisomerase III (EC 5.99.1.2), at 2,498,254 to 2,500,200
_2100
Position (kb)
2497
2498
2499 Strain fitness (log2 ratio)
-1
0
1 at 2496.714 kb on + strand, within Pf6N2E2_2097 at 2496.715 kb on - strand, within Pf6N2E2_2097 at 2496.715 kb on - strand, within Pf6N2E2_2097 at 2496.729 kb on + strand, within Pf6N2E2_2097 at 2496.729 kb on + strand, within Pf6N2E2_2097 at 2496.730 kb on - strand, within Pf6N2E2_2097 at 2496.730 kb on - strand, within Pf6N2E2_2097 at 2496.919 kb on + strand at 2496.919 kb on + strand at 2497.031 kb on - strand, within Pf6N2E2_2098 at 2497.120 kb on + strand, within Pf6N2E2_2098 at 2497.236 kb on + strand, within Pf6N2E2_2098 at 2497.236 kb on + strand, within Pf6N2E2_2098 at 2497.236 kb on + strand, within Pf6N2E2_2098 at 2497.237 kb on - strand, within Pf6N2E2_2098 at 2497.302 kb on + strand, within Pf6N2E2_2098 at 2497.303 kb on - strand, within Pf6N2E2_2098 at 2497.303 kb on - strand, within Pf6N2E2_2098 at 2497.582 kb on + strand at 2497.630 kb on + strand at 2497.631 kb on - strand at 2497.837 kb on - strand, within Pf6N2E2_2099 at 2497.976 kb on - strand at 2498.036 kb on - strand at 2498.036 kb on - strand at 2498.069 kb on + strand at 2498.070 kb on - strand at 2498.070 kb on - strand at 2498.178 kb on + strand at 2498.218 kb on + strand at 2498.219 kb on - strand at 2498.219 kb on - strand at 2498.263 kb on + strand at 2498.263 kb on + strand at 2498.263 kb on + strand at 2498.264 kb on - strand at 2498.264 kb on - strand at 2498.264 kb on - strand at 2498.264 kb on - strand at 2498.264 kb on - strand at 2498.264 kb on - strand at 2498.264 kb on - strand at 2498.264 kb on - strand at 2498.408 kb on - strand at 2498.408 kb on - strand at 2498.420 kb on - strand at 2498.500 kb on + strand, within Pf6N2E2_2100 at 2498.500 kb on + strand, within Pf6N2E2_2100 at 2498.500 kb on + strand, within Pf6N2E2_2100 at 2498.500 kb on + strand, within Pf6N2E2_2100 at 2498.501 kb on - strand, within Pf6N2E2_2100 at 2498.501 kb on - strand, within Pf6N2E2_2100 at 2498.711 kb on - strand, within Pf6N2E2_2100
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2 remove 2,496,714 + Pf6N2E2_2097 0.73 -0.1 2,496,715 - Pf6N2E2_2097 0.73 +0.9 2,496,715 - Pf6N2E2_2097 0.73 -1.1 2,496,729 + Pf6N2E2_2097 0.75 +0.9 2,496,729 + Pf6N2E2_2097 0.75 +0.9 2,496,730 - Pf6N2E2_2097 0.75 +0.9 2,496,730 - Pf6N2E2_2097 0.75 +0.9 2,496,919 + +0.3 2,496,919 + +0.9 2,497,031 - Pf6N2E2_2098 0.12 +0.9 2,497,120 + Pf6N2E2_2098 0.29 -0.7 2,497,236 + Pf6N2E2_2098 0.51 -0.8 2,497,236 + Pf6N2E2_2098 0.51 +0.9 2,497,236 + Pf6N2E2_2098 0.51 -0.1 2,497,237 - Pf6N2E2_2098 0.52 +0.9 2,497,302 + Pf6N2E2_2098 0.64 -0.1 2,497,303 - Pf6N2E2_2098 0.64 +0.9 2,497,303 - Pf6N2E2_2098 0.64 +0.9 2,497,582 + +0.9 2,497,630 + +0.9 2,497,631 - -0.7 2,497,837 - Pf6N2E2_2099 0.57 +0.9 2,497,976 - -0.5 2,498,036 - +0.9 2,498,036 - +0.9 2,498,069 + +0.9 2,498,070 - -0.7 2,498,070 - +0.9 2,498,178 + -0.1 2,498,218 + +0.9 2,498,219 - +0.9 2,498,219 - +0.9 2,498,263 + +0.9 2,498,263 + +0.9 2,498,263 + +0.9 2,498,264 - -0.1 2,498,264 - -0.1 2,498,264 - +0.9 2,498,264 - +0.9 2,498,264 - -0.1 2,498,264 - +0.9 2,498,264 - -0.1 2,498,264 - +0.9 2,498,408 - +0.9 2,498,408 - -0.9 2,498,420 - -1.1 2,498,500 + Pf6N2E2_2100 0.13 -0.8 2,498,500 + Pf6N2E2_2100 0.13 -0.7 2,498,500 + Pf6N2E2_2100 0.13 +0.5 2,498,500 + Pf6N2E2_2100 0.13 +0.3 2,498,501 - Pf6N2E2_2100 0.13 -0.8 2,498,501 - Pf6N2E2_2100 0.13 -0.7 2,498,711 - Pf6N2E2_2100 0.23 +0.9
Or see this region's nucleotide sequence