Experiment: Ying_OrganicAcid7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1838 and Pf6N2E2_1839 overlap by 1 nucleotides Pf6N2E2_1839 and Pf6N2E2_1840 overlap by 4 nucleotides Pf6N2E2_1840 and Pf6N2E2_1841 are separated by 10 nucleotides Pf6N2E2_1841 and Pf6N2E2_1842 are separated by 179 nucleotides
Pf6N2E2_1838 - 6-hexanolactone hydrolase, at 2,176,918 to 2,177,823
_1838
Pf6N2E2_1839 - 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (EC 1.1.1.-), at 2,177,823 to 2,178,557
_1839
Pf6N2E2_1840 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,178,554 to 2,179,366
_1840
Pf6N2E2_1841 - hypothetical protein, at 2,179,377 to 2,179,526
_1841
Pf6N2E2_1842 - Transcriptional regulator, TetR family, at 2,179,706 to 2,180,350
_1842
Position (kb)
2178
2179
2180 Strain fitness (log2 ratio)
-1
0
1 at 2177.603 kb on + strand, within Pf6N2E2_1838 at 2177.792 kb on - strand at 2178.115 kb on + strand, within Pf6N2E2_1839 at 2178.225 kb on + strand, within Pf6N2E2_1839 at 2178.254 kb on - strand, within Pf6N2E2_1839 at 2178.254 kb on - strand, within Pf6N2E2_1839 at 2178.302 kb on - strand, within Pf6N2E2_1839 at 2178.435 kb on - strand, within Pf6N2E2_1839 at 2178.500 kb on + strand at 2178.707 kb on - strand, within Pf6N2E2_1840 at 2179.087 kb on - strand, within Pf6N2E2_1840 at 2179.087 kb on - strand, within Pf6N2E2_1840 at 2179.105 kb on + strand, within Pf6N2E2_1840 at 2179.107 kb on + strand, within Pf6N2E2_1840 at 2179.108 kb on - strand, within Pf6N2E2_1840 at 2179.108 kb on - strand, within Pf6N2E2_1840 at 2179.141 kb on + strand, within Pf6N2E2_1840 at 2179.141 kb on + strand, within Pf6N2E2_1840 at 2179.142 kb on - strand, within Pf6N2E2_1840 at 2179.560 kb on + strand at 2179.566 kb on - strand at 2179.744 kb on + strand at 2179.773 kb on + strand, within Pf6N2E2_1842 at 2179.773 kb on + strand, within Pf6N2E2_1842 at 2179.774 kb on - strand, within Pf6N2E2_1842 at 2179.774 kb on - strand, within Pf6N2E2_1842 at 2179.996 kb on - strand, within Pf6N2E2_1842 at 2180.171 kb on - strand, within Pf6N2E2_1842 at 2180.171 kb on - strand, within Pf6N2E2_1842 at 2180.178 kb on - strand, within Pf6N2E2_1842 at 2180.224 kb on + strand, within Pf6N2E2_1842 at 2180.224 kb on + strand, within Pf6N2E2_1842 at 2180.225 kb on - strand, within Pf6N2E2_1842 at 2180.225 kb on - strand, within Pf6N2E2_1842 at 2180.241 kb on + strand, within Pf6N2E2_1842 at 2180.360 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2 remove 2,177,603 + Pf6N2E2_1838 0.76 +0.9 2,177,792 - +0.9 2,178,115 + Pf6N2E2_1839 0.40 +0.9 2,178,225 + Pf6N2E2_1839 0.55 +0.9 2,178,254 - Pf6N2E2_1839 0.59 -0.1 2,178,254 - Pf6N2E2_1839 0.59 +0.9 2,178,302 - Pf6N2E2_1839 0.65 +0.9 2,178,435 - Pf6N2E2_1839 0.83 -0.0 2,178,500 + +0.9 2,178,707 - Pf6N2E2_1840 0.19 +0.8 2,179,087 - Pf6N2E2_1840 0.66 +0.9 2,179,087 - Pf6N2E2_1840 0.66 +0.9 2,179,105 + Pf6N2E2_1840 0.68 -0.7 2,179,107 + Pf6N2E2_1840 0.68 +0.9 2,179,108 - Pf6N2E2_1840 0.68 +0.4 2,179,108 - Pf6N2E2_1840 0.68 +0.9 2,179,141 + Pf6N2E2_1840 0.72 -0.1 2,179,141 + Pf6N2E2_1840 0.72 -0.1 2,179,142 - Pf6N2E2_1840 0.72 -0.1 2,179,560 + -0.9 2,179,566 - -0.1 2,179,744 + -0.1 2,179,773 + Pf6N2E2_1842 0.10 +0.9 2,179,773 + Pf6N2E2_1842 0.10 +0.9 2,179,774 - Pf6N2E2_1842 0.11 +0.9 2,179,774 - Pf6N2E2_1842 0.11 +0.9 2,179,996 - Pf6N2E2_1842 0.45 +0.9 2,180,171 - Pf6N2E2_1842 0.72 -0.1 2,180,171 - Pf6N2E2_1842 0.72 +0.9 2,180,178 - Pf6N2E2_1842 0.73 +0.9 2,180,224 + Pf6N2E2_1842 0.80 +0.9 2,180,224 + Pf6N2E2_1842 0.80 +0.9 2,180,225 - Pf6N2E2_1842 0.80 -0.2 2,180,225 - Pf6N2E2_1842 0.80 -0.5 2,180,241 + Pf6N2E2_1842 0.83 -0.3 2,180,360 - -0.5
Or see this region's nucleotide sequence