Experiment: Ying_OrganicAcid7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1817 and Pf6N2E2_1818 are separated by 46 nucleotides Pf6N2E2_1818 and Pf6N2E2_1819 are separated by 137 nucleotides Pf6N2E2_1819 and Pf6N2E2_1820 are separated by 18 nucleotides Pf6N2E2_1820 and Pf6N2E2_1821 are separated by 64 nucleotides Pf6N2E2_1821 and Pf6N2E2_1822 are separated by 25 nucleotides
Pf6N2E2_1817 - hypothetical protein, at 2,151,486 to 2,151,908
_1817
Pf6N2E2_1818 - hypothetical protein, at 2,151,955 to 2,152,413
_1818
Pf6N2E2_1819 - 1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7), at 2,152,551 to 2,153,567
_1819
Pf6N2E2_1820 - hypothetical protein, at 2,153,586 to 2,153,702
_1820
Pf6N2E2_1821 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 2,153,767 to 2,154,276
_1821
Pf6N2E2_1822 - sensor histidine kinase, at 2,154,302 to 2,158,021
_1822
Position (kb)
2152
2153
2154 Strain fitness (log2 ratio)
-1
0
1 at 2151.559 kb on - strand, within Pf6N2E2_1817 at 2151.559 kb on - strand, within Pf6N2E2_1817 at 2151.559 kb on - strand, within Pf6N2E2_1817 at 2151.568 kb on - strand, within Pf6N2E2_1817 at 2151.845 kb on + strand, within Pf6N2E2_1817 at 2151.909 kb on - strand at 2152.181 kb on + strand, within Pf6N2E2_1818 at 2152.182 kb on - strand, within Pf6N2E2_1818 at 2152.271 kb on + strand, within Pf6N2E2_1818 at 2152.294 kb on + strand, within Pf6N2E2_1818 at 2152.295 kb on - strand, within Pf6N2E2_1818 at 2152.322 kb on + strand, within Pf6N2E2_1818 at 2152.322 kb on + strand, within Pf6N2E2_1818 at 2152.322 kb on + strand, within Pf6N2E2_1818 at 2152.323 kb on - strand, within Pf6N2E2_1818 at 2152.577 kb on - strand at 2152.687 kb on + strand, within Pf6N2E2_1819 at 2152.845 kb on - strand, within Pf6N2E2_1819 at 2152.994 kb on - strand, within Pf6N2E2_1819 at 2152.994 kb on - strand, within Pf6N2E2_1819 at 2153.190 kb on + strand, within Pf6N2E2_1819 at 2153.191 kb on - strand, within Pf6N2E2_1819 at 2153.239 kb on + strand, within Pf6N2E2_1819 at 2153.240 kb on - strand, within Pf6N2E2_1819 at 2153.398 kb on + strand, within Pf6N2E2_1819 at 2153.399 kb on - strand, within Pf6N2E2_1819 at 2153.465 kb on - strand, within Pf6N2E2_1819 at 2153.549 kb on + strand at 2153.585 kb on - strand at 2154.134 kb on - strand, within Pf6N2E2_1821 at 2154.199 kb on + strand, within Pf6N2E2_1821 at 2154.299 kb on + strand at 2154.311 kb on - strand at 2154.476 kb on - strand at 2154.533 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2 remove 2,151,559 - Pf6N2E2_1817 0.17 -0.1 2,151,559 - Pf6N2E2_1817 0.17 +0.9 2,151,559 - Pf6N2E2_1817 0.17 -0.5 2,151,568 - Pf6N2E2_1817 0.19 +0.9 2,151,845 + Pf6N2E2_1817 0.85 +0.9 2,151,909 - +0.9 2,152,181 + Pf6N2E2_1818 0.49 +0.9 2,152,182 - Pf6N2E2_1818 0.49 -1.0 2,152,271 + Pf6N2E2_1818 0.69 +0.9 2,152,294 + Pf6N2E2_1818 0.74 +0.9 2,152,295 - Pf6N2E2_1818 0.74 +0.9 2,152,322 + Pf6N2E2_1818 0.80 +0.9 2,152,322 + Pf6N2E2_1818 0.80 -0.5 2,152,322 + Pf6N2E2_1818 0.80 -1.6 2,152,323 - Pf6N2E2_1818 0.80 -0.7 2,152,577 - +0.9 2,152,687 + Pf6N2E2_1819 0.13 +0.5 2,152,845 - Pf6N2E2_1819 0.29 +0.9 2,152,994 - Pf6N2E2_1819 0.44 +0.1 2,152,994 - Pf6N2E2_1819 0.44 -1.6 2,153,190 + Pf6N2E2_1819 0.63 +0.9 2,153,191 - Pf6N2E2_1819 0.63 +0.9 2,153,239 + Pf6N2E2_1819 0.68 +1.5 2,153,240 - Pf6N2E2_1819 0.68 +0.9 2,153,398 + Pf6N2E2_1819 0.83 +0.9 2,153,399 - Pf6N2E2_1819 0.83 +0.9 2,153,465 - Pf6N2E2_1819 0.90 -0.1 2,153,549 + -0.1 2,153,585 - -0.7 2,154,134 - Pf6N2E2_1821 0.72 +0.9 2,154,199 + Pf6N2E2_1821 0.85 -0.1 2,154,299 + +0.9 2,154,311 - +0.9 2,154,476 - -0.7 2,154,533 + +0.9
Or see this region's nucleotide sequence