Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1819

Experiment: Ying_OrganicAcid7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1817 and Pf6N2E2_1818 are separated by 46 nucleotidesPf6N2E2_1818 and Pf6N2E2_1819 are separated by 137 nucleotidesPf6N2E2_1819 and Pf6N2E2_1820 are separated by 18 nucleotidesPf6N2E2_1820 and Pf6N2E2_1821 are separated by 64 nucleotidesPf6N2E2_1821 and Pf6N2E2_1822 are separated by 25 nucleotides Pf6N2E2_1817 - hypothetical protein, at 2,151,486 to 2,151,908 _1817 Pf6N2E2_1818 - hypothetical protein, at 2,151,955 to 2,152,413 _1818 Pf6N2E2_1819 - 1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7), at 2,152,551 to 2,153,567 _1819 Pf6N2E2_1820 - hypothetical protein, at 2,153,586 to 2,153,702 _1820 Pf6N2E2_1821 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 2,153,767 to 2,154,276 _1821 Pf6N2E2_1822 - sensor histidine kinase, at 2,154,302 to 2,158,021 _1822 Position (kb) 2152 2153 2154Strain fitness (log2 ratio) -1 0 1at 2151.559 kb on - strand, within Pf6N2E2_1817at 2151.559 kb on - strand, within Pf6N2E2_1817at 2151.559 kb on - strand, within Pf6N2E2_1817at 2151.568 kb on - strand, within Pf6N2E2_1817at 2151.845 kb on + strand, within Pf6N2E2_1817at 2151.909 kb on - strandat 2152.181 kb on + strand, within Pf6N2E2_1818at 2152.182 kb on - strand, within Pf6N2E2_1818at 2152.271 kb on + strand, within Pf6N2E2_1818at 2152.294 kb on + strand, within Pf6N2E2_1818at 2152.295 kb on - strand, within Pf6N2E2_1818at 2152.322 kb on + strand, within Pf6N2E2_1818at 2152.322 kb on + strand, within Pf6N2E2_1818at 2152.322 kb on + strand, within Pf6N2E2_1818at 2152.323 kb on - strand, within Pf6N2E2_1818at 2152.577 kb on - strandat 2152.687 kb on + strand, within Pf6N2E2_1819at 2152.845 kb on - strand, within Pf6N2E2_1819at 2152.994 kb on - strand, within Pf6N2E2_1819at 2152.994 kb on - strand, within Pf6N2E2_1819at 2153.190 kb on + strand, within Pf6N2E2_1819at 2153.191 kb on - strand, within Pf6N2E2_1819at 2153.239 kb on + strand, within Pf6N2E2_1819at 2153.240 kb on - strand, within Pf6N2E2_1819at 2153.398 kb on + strand, within Pf6N2E2_1819at 2153.399 kb on - strand, within Pf6N2E2_1819at 2153.465 kb on - strand, within Pf6N2E2_1819at 2153.549 kb on + strandat 2153.585 kb on - strandat 2154.134 kb on - strand, within Pf6N2E2_1821at 2154.199 kb on + strand, within Pf6N2E2_1821at 2154.299 kb on + strandat 2154.311 kb on - strandat 2154.476 kb on - strandat 2154.533 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2
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2,151,559 - Pf6N2E2_1817 0.17 -0.1
2,151,559 - Pf6N2E2_1817 0.17 +0.9
2,151,559 - Pf6N2E2_1817 0.17 -0.5
2,151,568 - Pf6N2E2_1817 0.19 +0.9
2,151,845 + Pf6N2E2_1817 0.85 +0.9
2,151,909 - +0.9
2,152,181 + Pf6N2E2_1818 0.49 +0.9
2,152,182 - Pf6N2E2_1818 0.49 -1.0
2,152,271 + Pf6N2E2_1818 0.69 +0.9
2,152,294 + Pf6N2E2_1818 0.74 +0.9
2,152,295 - Pf6N2E2_1818 0.74 +0.9
2,152,322 + Pf6N2E2_1818 0.80 +0.9
2,152,322 + Pf6N2E2_1818 0.80 -0.5
2,152,322 + Pf6N2E2_1818 0.80 -1.6
2,152,323 - Pf6N2E2_1818 0.80 -0.7
2,152,577 - +0.9
2,152,687 + Pf6N2E2_1819 0.13 +0.5
2,152,845 - Pf6N2E2_1819 0.29 +0.9
2,152,994 - Pf6N2E2_1819 0.44 +0.1
2,152,994 - Pf6N2E2_1819 0.44 -1.6
2,153,190 + Pf6N2E2_1819 0.63 +0.9
2,153,191 - Pf6N2E2_1819 0.63 +0.9
2,153,239 + Pf6N2E2_1819 0.68 +1.5
2,153,240 - Pf6N2E2_1819 0.68 +0.9
2,153,398 + Pf6N2E2_1819 0.83 +0.9
2,153,399 - Pf6N2E2_1819 0.83 +0.9
2,153,465 - Pf6N2E2_1819 0.90 -0.1
2,153,549 + -0.1
2,153,585 - -0.7
2,154,134 - Pf6N2E2_1821 0.72 +0.9
2,154,199 + Pf6N2E2_1821 0.85 -0.1
2,154,299 + +0.9
2,154,311 - +0.9
2,154,476 - -0.7
2,154,533 + +0.9

Or see this region's nucleotide sequence