Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1500

Experiment: Ying_OrganicAcid7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1499 and Pf6N2E2_1500 are separated by 240 nucleotidesPf6N2E2_1500 and Pf6N2E2_1501 are separated by 34 nucleotidesPf6N2E2_1501 and Pf6N2E2_1502 are separated by 111 nucleotidesPf6N2E2_1502 and Pf6N2E2_1503 are separated by 20 nucleotides Pf6N2E2_1499 - ABC transporter amino acid-binding protein, at 1,753,015 to 1,753,857 _1499 Pf6N2E2_1500 - LysR family transcriptional regulator lrhA, at 1,754,098 to 1,754,955 _1500 Pf6N2E2_1501 - Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) (EC 3.6.1.17), at 1,754,990 to 1,755,433 _1501 Pf6N2E2_1502 - Two-component system regulatory protein, at 1,755,545 to 1,755,922 _1502 Pf6N2E2_1503 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 1,755,943 to 1,758,126 _1503 Position (kb) 1754 1755Strain fitness (log2 ratio) -1 0 1at 1753.296 kb on + strand, within Pf6N2E2_1499at 1753.379 kb on + strand, within Pf6N2E2_1499at 1753.380 kb on - strand, within Pf6N2E2_1499at 1753.453 kb on + strand, within Pf6N2E2_1499at 1753.479 kb on - strand, within Pf6N2E2_1499at 1753.862 kb on - strandat 1753.947 kb on - strandat 1753.957 kb on + strandat 1754.321 kb on + strand, within Pf6N2E2_1500at 1754.611 kb on + strand, within Pf6N2E2_1500at 1754.612 kb on - strand, within Pf6N2E2_1500at 1754.669 kb on - strand, within Pf6N2E2_1500at 1754.725 kb on + strand, within Pf6N2E2_1500at 1754.866 kb on + strand, within Pf6N2E2_1500at 1754.866 kb on + strand, within Pf6N2E2_1500at 1754.866 kb on + strand, within Pf6N2E2_1500at 1754.866 kb on + strand, within Pf6N2E2_1500at 1754.866 kb on + strandat 1754.866 kb on + strand, within Pf6N2E2_1500at 1754.867 kb on - strand, within Pf6N2E2_1500at 1754.867 kb on - strand, within Pf6N2E2_1500at 1754.889 kb on + strandat 1754.890 kb on - strandat 1754.904 kb on - strandat 1754.918 kb on - strandat 1754.918 kb on - strandat 1754.918 kb on - strandat 1754.979 kb on - strandat 1755.123 kb on + strand, within Pf6N2E2_1501at 1755.123 kb on + strand, within Pf6N2E2_1501at 1755.123 kb on + strand, within Pf6N2E2_1501at 1755.124 kb on - strand, within Pf6N2E2_1501at 1755.355 kb on - strand, within Pf6N2E2_1501at 1755.414 kb on + strandat 1755.414 kb on + strandat 1755.414 kb on + strandat 1755.454 kb on + strandat 1755.454 kb on + strandat 1755.486 kb on + strandat 1755.486 kb on + strandat 1755.486 kb on + strandat 1755.486 kb on + strandat 1755.487 kb on - strandat 1755.715 kb on + strand, within Pf6N2E2_1502at 1755.715 kb on + strand, within Pf6N2E2_1502at 1755.932 kb on + strandat 1755.933 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2
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1,753,296 + Pf6N2E2_1499 0.33 +0.8
1,753,379 + Pf6N2E2_1499 0.43 +0.8
1,753,380 - Pf6N2E2_1499 0.43 +0.8
1,753,453 + Pf6N2E2_1499 0.52 -0.5
1,753,479 - Pf6N2E2_1499 0.55 -0.2
1,753,862 - -0.2
1,753,947 - -0.2
1,753,957 + +0.8
1,754,321 + Pf6N2E2_1500 0.26 +0.8
1,754,611 + Pf6N2E2_1500 0.60 +0.8
1,754,612 - Pf6N2E2_1500 0.60 -0.2
1,754,669 - Pf6N2E2_1500 0.67 +0.8
1,754,725 + Pf6N2E2_1500 0.73 -1.2
1,754,866 + Pf6N2E2_1500 0.90 +0.8
1,754,866 + Pf6N2E2_1500 0.90 +0.8
1,754,866 + Pf6N2E2_1500 0.90 +0.8
1,754,866 + Pf6N2E2_1500 0.90 -0.8
1,754,866 + +0.8
1,754,866 + Pf6N2E2_1500 0.90 -0.2
1,754,867 - Pf6N2E2_1500 0.90 -0.1
1,754,867 - Pf6N2E2_1500 0.90 +0.3
1,754,889 + +0.8
1,754,890 - -0.5
1,754,904 - +0.8
1,754,918 - -0.6
1,754,918 - +0.8
1,754,918 - +0.8
1,754,979 - -0.7
1,755,123 + Pf6N2E2_1501 0.30 +0.8
1,755,123 + Pf6N2E2_1501 0.30 +0.8
1,755,123 + Pf6N2E2_1501 0.30 +0.8
1,755,124 - Pf6N2E2_1501 0.30 +0.8
1,755,355 - Pf6N2E2_1501 0.82 +0.8
1,755,414 + -0.2
1,755,414 + -0.3
1,755,414 + -0.2
1,755,454 + -0.8
1,755,454 + -0.8
1,755,486 + +0.8
1,755,486 + -0.6
1,755,486 + -0.7
1,755,486 + +0.8
1,755,487 - +1.4
1,755,715 + Pf6N2E2_1502 0.45 -0.8
1,755,715 + Pf6N2E2_1502 0.45 -0.8
1,755,932 + -0.8
1,755,933 - -0.3

Or see this region's nucleotide sequence