Experiment: Ying_OrganicAcid7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1430 and Pf6N2E2_1431 are separated by 72 nucleotides Pf6N2E2_1431 and Pf6N2E2_1432 are separated by 11 nucleotides Pf6N2E2_1432 and Pf6N2E2_1433 are separated by 67 nucleotides
Pf6N2E2_1430 - Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein, at 1,679,300 to 1,680,625
_1430
Pf6N2E2_1431 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 1,680,698 to 1,681,765
_1431
Pf6N2E2_1432 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 1,681,777 to 1,682,658
_1432
Pf6N2E2_1433 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 1,682,726 to 1,683,508
_1433
Position (kb)
1680
1681
1682 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1679.720 kb on + strand, within Pf6N2E2_1430 at 1679.721 kb on - strand, within Pf6N2E2_1430 at 1679.957 kb on - strand, within Pf6N2E2_1430 at 1680.024 kb on + strand, within Pf6N2E2_1430 at 1680.084 kb on + strand, within Pf6N2E2_1430 at 1680.085 kb on - strand, within Pf6N2E2_1430 at 1680.144 kb on + strand, within Pf6N2E2_1430 at 1680.145 kb on - strand, within Pf6N2E2_1430 at 1680.148 kb on + strand, within Pf6N2E2_1430 at 1680.148 kb on + strand, within Pf6N2E2_1430 at 1680.149 kb on - strand, within Pf6N2E2_1430 at 1680.149 kb on - strand, within Pf6N2E2_1430 at 1680.149 kb on - strand, within Pf6N2E2_1430 at 1680.299 kb on - strand, within Pf6N2E2_1430 at 1680.363 kb on + strand, within Pf6N2E2_1430 at 1680.364 kb on - strand, within Pf6N2E2_1430 at 1680.391 kb on - strand, within Pf6N2E2_1430 at 1680.391 kb on - strand, within Pf6N2E2_1430 at 1680.391 kb on - strand, within Pf6N2E2_1430 at 1680.454 kb on - strand, within Pf6N2E2_1430 at 1680.454 kb on - strand, within Pf6N2E2_1430 at 1680.462 kb on + strand, within Pf6N2E2_1430 at 1680.462 kb on + strand, within Pf6N2E2_1430 at 1680.463 kb on - strand, within Pf6N2E2_1430 at 1680.537 kb on + strand at 1680.537 kb on + strand at 1680.538 kb on - strand at 1680.538 kb on - strand at 1680.538 kb on - strand at 1680.538 kb on - strand at 1680.641 kb on - strand at 1680.641 kb on - strand at 1680.655 kb on - strand at 1681.371 kb on + strand, within Pf6N2E2_1431 at 1681.372 kb on - strand, within Pf6N2E2_1431 at 1681.492 kb on - strand, within Pf6N2E2_1431 at 1681.779 kb on - strand at 1681.895 kb on + strand, within Pf6N2E2_1432 at 1681.896 kb on - strand, within Pf6N2E2_1432 at 1681.963 kb on - strand, within Pf6N2E2_1432 at 1682.351 kb on + strand, within Pf6N2E2_1432 at 1682.351 kb on + strand, within Pf6N2E2_1432 at 1682.352 kb on - strand, within Pf6N2E2_1432 at 1682.573 kb on + strand at 1682.606 kb on + strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.638 kb on + strand at 1682.638 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2 remove 1,679,720 + Pf6N2E2_1430 0.32 +0.8 1,679,721 - Pf6N2E2_1430 0.32 -0.8 1,679,957 - Pf6N2E2_1430 0.50 +0.8 1,680,024 + Pf6N2E2_1430 0.55 +0.8 1,680,084 + Pf6N2E2_1430 0.59 +0.8 1,680,085 - Pf6N2E2_1430 0.59 -0.2 1,680,144 + Pf6N2E2_1430 0.64 -0.2 1,680,145 - Pf6N2E2_1430 0.64 +0.8 1,680,148 + Pf6N2E2_1430 0.64 +0.8 1,680,148 + Pf6N2E2_1430 0.64 +0.8 1,680,149 - Pf6N2E2_1430 0.64 -0.9 1,680,149 - Pf6N2E2_1430 0.64 +0.8 1,680,149 - Pf6N2E2_1430 0.64 +0.8 1,680,299 - Pf6N2E2_1430 0.75 +0.8 1,680,363 + Pf6N2E2_1430 0.80 +0.8 1,680,364 - Pf6N2E2_1430 0.80 +0.8 1,680,391 - Pf6N2E2_1430 0.82 -0.1 1,680,391 - Pf6N2E2_1430 0.82 +0.8 1,680,391 - Pf6N2E2_1430 0.82 -0.2 1,680,454 - Pf6N2E2_1430 0.87 -0.2 1,680,454 - Pf6N2E2_1430 0.87 +0.3 1,680,462 + Pf6N2E2_1430 0.88 +0.8 1,680,462 + Pf6N2E2_1430 0.88 -0.8 1,680,463 - Pf6N2E2_1430 0.88 -0.2 1,680,537 + -0.4 1,680,537 + +0.8 1,680,538 - +0.8 1,680,538 - -0.3 1,680,538 - -0.6 1,680,538 - +0.8 1,680,641 - -0.8 1,680,641 - +0.8 1,680,655 - -1.0 1,681,371 + Pf6N2E2_1431 0.63 +0.8 1,681,372 - Pf6N2E2_1431 0.63 -1.8 1,681,492 - Pf6N2E2_1431 0.74 -0.2 1,681,779 - -0.2 1,681,895 + Pf6N2E2_1432 0.13 +0.8 1,681,896 - Pf6N2E2_1432 0.13 +0.8 1,681,963 - Pf6N2E2_1432 0.21 -0.2 1,682,351 + Pf6N2E2_1432 0.65 -0.2 1,682,351 + Pf6N2E2_1432 0.65 -0.3 1,682,352 - Pf6N2E2_1432 0.65 -0.4 1,682,573 + -0.5 1,682,606 + +0.8 1,682,607 - -0.8 1,682,607 - -0.6 1,682,607 - -0.2 1,682,607 - +0.8 1,682,607 - +2.4 1,682,607 - -0.2 1,682,638 + +0.8 1,682,638 + -0.2
Or see this region's nucleotide sequence