Experiment: Ying_OrganicAcid7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1202 and Pf6N2E2_1203 are separated by 139 nucleotides Pf6N2E2_1203 and Pf6N2E2_1204 are separated by 12 nucleotides
Pf6N2E2_1202 - hypothetical protein, at 1,404,775 to 1,408,689
_1202
Pf6N2E2_1203 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 1,408,829 to 1,409,302
_1203
Pf6N2E2_1204 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 1,409,315 to 1,411,552
_1204
Position (kb)
1408
1409
1410 Strain fitness (log2 ratio)
-1
0
1 at 1407.927 kb on + strand, within Pf6N2E2_1202 at 1408.012 kb on + strand, within Pf6N2E2_1202 at 1408.012 kb on + strand, within Pf6N2E2_1202 at 1408.012 kb on + strand, within Pf6N2E2_1202 at 1408.013 kb on - strand, within Pf6N2E2_1202 at 1408.013 kb on - strand, within Pf6N2E2_1202 at 1408.013 kb on - strand, within Pf6N2E2_1202 at 1408.013 kb on - strand, within Pf6N2E2_1202 at 1408.017 kb on - strand, within Pf6N2E2_1202 at 1408.112 kb on + strand, within Pf6N2E2_1202 at 1408.265 kb on + strand, within Pf6N2E2_1202 at 1408.266 kb on - strand, within Pf6N2E2_1202 at 1408.290 kb on + strand, within Pf6N2E2_1202 at 1408.436 kb on - strand at 1408.436 kb on - strand at 1408.441 kb on + strand at 1408.442 kb on - strand at 1408.703 kb on + strand at 1408.780 kb on + strand at 1408.780 kb on + strand at 1408.788 kb on + strand at 1408.789 kb on - strand at 1408.789 kb on - strand at 1408.790 kb on + strand at 1408.829 kb on - strand at 1409.252 kb on + strand, within Pf6N2E2_1203 at 1409.253 kb on - strand, within Pf6N2E2_1203 at 1409.253 kb on - strand, within Pf6N2E2_1203 at 1409.253 kb on - strand, within Pf6N2E2_1203 at 1409.253 kb on - strand, within Pf6N2E2_1203 at 1409.253 kb on - strand, within Pf6N2E2_1203 at 1409.687 kb on + strand, within Pf6N2E2_1204 at 1409.748 kb on - strand, within Pf6N2E2_1204 at 1409.748 kb on - strand, within Pf6N2E2_1204 at 1410.139 kb on - strand, within Pf6N2E2_1204 at 1410.203 kb on + strand, within Pf6N2E2_1204 at 1410.204 kb on - strand, within Pf6N2E2_1204
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2 remove 1,407,927 + Pf6N2E2_1202 0.81 +0.8 1,408,012 + Pf6N2E2_1202 0.83 +0.8 1,408,012 + Pf6N2E2_1202 0.83 +0.8 1,408,012 + Pf6N2E2_1202 0.83 +0.8 1,408,013 - Pf6N2E2_1202 0.83 +0.8 1,408,013 - Pf6N2E2_1202 0.83 -0.1 1,408,013 - Pf6N2E2_1202 0.83 -1.2 1,408,013 - Pf6N2E2_1202 0.83 +0.8 1,408,017 - Pf6N2E2_1202 0.83 -0.8 1,408,112 + Pf6N2E2_1202 0.85 -0.2 1,408,265 + Pf6N2E2_1202 0.89 +0.8 1,408,266 - Pf6N2E2_1202 0.89 +0.8 1,408,290 + Pf6N2E2_1202 0.90 +0.8 1,408,436 - -0.2 1,408,436 - +0.8 1,408,441 + -0.2 1,408,442 - +0.8 1,408,703 + +0.8 1,408,780 + -0.8 1,408,780 + -0.8 1,408,788 + -1.4 1,408,789 - +0.8 1,408,789 - -0.2 1,408,790 + +0.8 1,408,829 - +0.8 1,409,252 + Pf6N2E2_1203 0.89 +0.8 1,409,253 - Pf6N2E2_1203 0.89 +0.8 1,409,253 - Pf6N2E2_1203 0.89 -1.5 1,409,253 - Pf6N2E2_1203 0.89 +0.8 1,409,253 - Pf6N2E2_1203 0.89 -0.2 1,409,253 - Pf6N2E2_1203 0.89 -0.2 1,409,687 + Pf6N2E2_1204 0.17 +0.8 1,409,748 - Pf6N2E2_1204 0.19 +0.8 1,409,748 - Pf6N2E2_1204 0.19 +0.8 1,410,139 - Pf6N2E2_1204 0.37 -0.8 1,410,203 + Pf6N2E2_1204 0.40 +0.8 1,410,204 - Pf6N2E2_1204 0.40 +1.0
Or see this region's nucleotide sequence