Experiment: Ying_OrganicAcid7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1001 and Pf6N2E2_1002 are separated by 92 nucleotides Pf6N2E2_1002 and Pf6N2E2_1003 are separated by 68 nucleotides
Pf6N2E2_1001 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 1,170,296 to 1,171,150
_1001
Pf6N2E2_1002 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 1,171,243 to 1,172,244
_1002
Pf6N2E2_1003 - Fucose permease, at 1,172,313 to 1,173,695
_1003
Position (kb)
1171
1172
1173 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1170.245 kb on + strand at 1170.245 kb on + strand at 1170.246 kb on - strand at 1170.291 kb on + strand at 1170.292 kb on - strand at 1170.330 kb on - strand at 1170.330 kb on - strand at 1170.353 kb on + strand at 1170.402 kb on + strand, within Pf6N2E2_1001 at 1170.403 kb on - strand, within Pf6N2E2_1001 at 1170.425 kb on + strand, within Pf6N2E2_1001 at 1170.587 kb on - strand, within Pf6N2E2_1001 at 1170.587 kb on - strand, within Pf6N2E2_1001 at 1170.657 kb on + strand, within Pf6N2E2_1001 at 1170.658 kb on - strand, within Pf6N2E2_1001 at 1170.676 kb on + strand, within Pf6N2E2_1001 at 1170.728 kb on + strand, within Pf6N2E2_1001 at 1170.728 kb on + strand, within Pf6N2E2_1001 at 1170.729 kb on - strand, within Pf6N2E2_1001 at 1170.763 kb on + strand, within Pf6N2E2_1001 at 1170.776 kb on - strand, within Pf6N2E2_1001 at 1170.776 kb on - strand, within Pf6N2E2_1001 at 1170.934 kb on + strand, within Pf6N2E2_1001 at 1170.935 kb on - strand, within Pf6N2E2_1001 at 1170.935 kb on - strand, within Pf6N2E2_1001 at 1170.938 kb on + strand at 1171.018 kb on + strand, within Pf6N2E2_1001 at 1171.018 kb on + strand, within Pf6N2E2_1001 at 1171.021 kb on + strand, within Pf6N2E2_1001 at 1171.021 kb on + strand, within Pf6N2E2_1001 at 1171.021 kb on + strand, within Pf6N2E2_1001 at 1171.028 kb on - strand, within Pf6N2E2_1001 at 1171.148 kb on + strand at 1171.148 kb on + strand at 1171.148 kb on + strand at 1171.154 kb on + strand at 1171.154 kb on + strand at 1171.154 kb on + strand at 1171.219 kb on + strand at 1171.239 kb on + strand at 1171.239 kb on + strand at 1171.239 kb on + strand at 1171.239 kb on + strand at 1171.239 kb on + strand at 1171.240 kb on - strand at 1171.240 kb on - strand at 1171.334 kb on + strand at 1171.334 kb on + strand at 1171.335 kb on - strand at 1171.396 kb on + strand, within Pf6N2E2_1002 at 1171.396 kb on + strand, within Pf6N2E2_1002 at 1171.402 kb on + strand, within Pf6N2E2_1002 at 1171.402 kb on + strand, within Pf6N2E2_1002 at 1171.403 kb on - strand, within Pf6N2E2_1002 at 1171.422 kb on + strand, within Pf6N2E2_1002 at 1171.481 kb on + strand, within Pf6N2E2_1002 at 1171.509 kb on + strand, within Pf6N2E2_1002 at 1171.537 kb on + strand, within Pf6N2E2_1002 at 1171.537 kb on + strand, within Pf6N2E2_1002 at 1171.758 kb on + strand, within Pf6N2E2_1002 at 1171.758 kb on + strand, within Pf6N2E2_1002 at 1171.758 kb on + strand, within Pf6N2E2_1002 at 1171.758 kb on + strand, within Pf6N2E2_1002 at 1171.890 kb on + strand, within Pf6N2E2_1002 at 1171.890 kb on + strand, within Pf6N2E2_1002 at 1171.937 kb on + strand, within Pf6N2E2_1002 at 1171.937 kb on + strand, within Pf6N2E2_1002 at 1171.937 kb on + strand, within Pf6N2E2_1002 at 1171.937 kb on + strand, within Pf6N2E2_1002 at 1171.937 kb on + strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.939 kb on + strand, within Pf6N2E2_1002 at 1171.939 kb on + strand, within Pf6N2E2_1002 at 1171.940 kb on - strand, within Pf6N2E2_1002 at 1172.004 kb on + strand, within Pf6N2E2_1002 at 1172.005 kb on - strand, within Pf6N2E2_1002 at 1172.005 kb on - strand, within Pf6N2E2_1002 at 1172.092 kb on + strand, within Pf6N2E2_1002 at 1172.092 kb on + strand, within Pf6N2E2_1002 at 1172.093 kb on - strand, within Pf6N2E2_1002 at 1172.093 kb on - strand, within Pf6N2E2_1002 at 1172.133 kb on + strand, within Pf6N2E2_1002 at 1172.134 kb on - strand, within Pf6N2E2_1002 at 1172.225 kb on + strand at 1172.225 kb on + strand at 1172.225 kb on + strand at 1172.225 kb on + strand at 1172.225 kb on + strand at 1172.226 kb on - strand at 1172.243 kb on - strand at 1172.247 kb on + strand at 1172.248 kb on - strand at 1172.276 kb on - strand at 1172.276 kb on - strand at 1172.330 kb on - strand at 1172.339 kb on + strand at 1172.340 kb on - strand at 1172.444 kb on + strand at 1172.444 kb on + strand at 1172.508 kb on - strand, within Pf6N2E2_1003 at 1172.594 kb on + strand, within Pf6N2E2_1003 at 1172.594 kb on + strand, within Pf6N2E2_1003 at 1172.595 kb on - strand, within Pf6N2E2_1003 at 1172.722 kb on + strand, within Pf6N2E2_1003 at 1172.723 kb on - strand, within Pf6N2E2_1003 at 1172.775 kb on - strand, within Pf6N2E2_1003 at 1172.927 kb on - strand, within Pf6N2E2_1003 at 1173.009 kb on + strand, within Pf6N2E2_1003 at 1173.009 kb on + strand, within Pf6N2E2_1003 at 1173.010 kb on - strand, within Pf6N2E2_1003 at 1173.010 kb on - strand, within Pf6N2E2_1003 at 1173.010 kb on - strand, within Pf6N2E2_1003 at 1173.125 kb on - strand, within Pf6N2E2_1003 at 1173.125 kb on - strand, within Pf6N2E2_1003 at 1173.125 kb on - strand, within Pf6N2E2_1003 at 1173.189 kb on + strand, within Pf6N2E2_1003 at 1173.189 kb on + strand, within Pf6N2E2_1003 at 1173.190 kb on - strand, within Pf6N2E2_1003 at 1173.219 kb on + strand, within Pf6N2E2_1003
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2 remove 1,170,245 + -0.7 1,170,245 + +0.8 1,170,246 - +0.8 1,170,291 + -0.2 1,170,292 - +0.8 1,170,330 - +0.8 1,170,330 - +0.3 1,170,353 + -1.2 1,170,402 + Pf6N2E2_1001 0.12 +0.8 1,170,403 - Pf6N2E2_1001 0.13 -1.2 1,170,425 + Pf6N2E2_1001 0.15 +0.8 1,170,587 - Pf6N2E2_1001 0.34 -0.7 1,170,587 - Pf6N2E2_1001 0.34 +0.8 1,170,657 + Pf6N2E2_1001 0.42 -0.5 1,170,658 - Pf6N2E2_1001 0.42 -1.0 1,170,676 + Pf6N2E2_1001 0.44 +0.8 1,170,728 + Pf6N2E2_1001 0.51 +0.8 1,170,728 + Pf6N2E2_1001 0.51 +0.8 1,170,729 - Pf6N2E2_1001 0.51 +0.3 1,170,763 + Pf6N2E2_1001 0.55 +0.8 1,170,776 - Pf6N2E2_1001 0.56 +0.6 1,170,776 - Pf6N2E2_1001 0.56 -0.2 1,170,934 + Pf6N2E2_1001 0.75 +0.8 1,170,935 - Pf6N2E2_1001 0.75 +0.8 1,170,935 - Pf6N2E2_1001 0.75 +0.8 1,170,938 + +0.8 1,171,018 + Pf6N2E2_1001 0.84 +0.8 1,171,018 + Pf6N2E2_1001 0.84 +0.8 1,171,021 + Pf6N2E2_1001 0.85 -0.7 1,171,021 + Pf6N2E2_1001 0.85 -0.3 1,171,021 + Pf6N2E2_1001 0.85 -0.3 1,171,028 - Pf6N2E2_1001 0.86 -1.3 1,171,148 + +0.8 1,171,148 + -0.2 1,171,148 + -0.2 1,171,154 + +0.8 1,171,154 + +0.8 1,171,154 + +0.8 1,171,219 + +0.1 1,171,239 + -0.2 1,171,239 + +0.8 1,171,239 + -0.4 1,171,239 + +0.8 1,171,239 + -1.2 1,171,240 - -0.2 1,171,240 - +0.8 1,171,334 + -0.6 1,171,334 + -0.7 1,171,335 - +0.8 1,171,396 + Pf6N2E2_1002 0.15 +0.8 1,171,396 + Pf6N2E2_1002 0.15 -0.2 1,171,402 + Pf6N2E2_1002 0.16 +0.8 1,171,402 + Pf6N2E2_1002 0.16 +0.8 1,171,403 - Pf6N2E2_1002 0.16 +0.8 1,171,422 + Pf6N2E2_1002 0.18 -0.2 1,171,481 + Pf6N2E2_1002 0.24 -0.2 1,171,509 + Pf6N2E2_1002 0.27 +0.8 1,171,537 + Pf6N2E2_1002 0.29 -0.5 1,171,537 + Pf6N2E2_1002 0.29 +0.8 1,171,758 + Pf6N2E2_1002 0.51 -0.2 1,171,758 + Pf6N2E2_1002 0.51 -1.7 1,171,758 + Pf6N2E2_1002 0.51 -0.2 1,171,758 + Pf6N2E2_1002 0.51 +0.8 1,171,890 + Pf6N2E2_1002 0.65 +0.8 1,171,890 + Pf6N2E2_1002 0.65 +0.8 1,171,937 + Pf6N2E2_1002 0.69 -1.3 1,171,937 + Pf6N2E2_1002 0.69 -0.2 1,171,937 + Pf6N2E2_1002 0.69 +0.8 1,171,937 + Pf6N2E2_1002 0.69 -0.2 1,171,937 + Pf6N2E2_1002 0.69 -0.2 1,171,938 - Pf6N2E2_1002 0.69 +0.8 1,171,938 - Pf6N2E2_1002 0.69 -0.2 1,171,938 - +0.8 1,171,938 - Pf6N2E2_1002 0.69 +0.8 1,171,938 - Pf6N2E2_1002 0.69 -0.2 1,171,938 - Pf6N2E2_1002 0.69 -0.9 1,171,938 - Pf6N2E2_1002 0.69 +0.8 1,171,938 - Pf6N2E2_1002 0.69 -0.2 1,171,939 + Pf6N2E2_1002 0.69 +0.8 1,171,939 + Pf6N2E2_1002 0.69 +0.8 1,171,940 - Pf6N2E2_1002 0.70 +0.8 1,172,004 + Pf6N2E2_1002 0.76 +0.8 1,172,005 - Pf6N2E2_1002 0.76 +0.8 1,172,005 - Pf6N2E2_1002 0.76 +0.8 1,172,092 + Pf6N2E2_1002 0.85 -0.2 1,172,092 + Pf6N2E2_1002 0.85 +0.8 1,172,093 - Pf6N2E2_1002 0.85 +0.8 1,172,093 - Pf6N2E2_1002 0.85 +3.2 1,172,133 + Pf6N2E2_1002 0.89 -0.2 1,172,134 - Pf6N2E2_1002 0.89 -0.7 1,172,225 + -0.7 1,172,225 + +0.8 1,172,225 + -0.4 1,172,225 + +0.8 1,172,225 + -0.6 1,172,226 - +0.8 1,172,243 - +0.8 1,172,247 + -0.6 1,172,248 - +0.8 1,172,276 - +0.8 1,172,276 - -1.2 1,172,330 - -0.2 1,172,339 + +0.4 1,172,340 - +0.8 1,172,444 + +0.8 1,172,444 + -1.4 1,172,508 - Pf6N2E2_1003 0.14 +0.8 1,172,594 + Pf6N2E2_1003 0.20 -2.0 1,172,594 + Pf6N2E2_1003 0.20 +0.8 1,172,595 - Pf6N2E2_1003 0.20 +0.8 1,172,722 + Pf6N2E2_1003 0.30 -0.3 1,172,723 - Pf6N2E2_1003 0.30 -0.7 1,172,775 - Pf6N2E2_1003 0.33 +0.8 1,172,927 - Pf6N2E2_1003 0.44 +0.8 1,173,009 + Pf6N2E2_1003 0.50 +0.8 1,173,009 + Pf6N2E2_1003 0.50 +0.8 1,173,010 - Pf6N2E2_1003 0.50 -0.2 1,173,010 - Pf6N2E2_1003 0.50 +0.8 1,173,010 - Pf6N2E2_1003 0.50 +0.0 1,173,125 - Pf6N2E2_1003 0.59 +0.8 1,173,125 - Pf6N2E2_1003 0.59 +0.8 1,173,125 - Pf6N2E2_1003 0.59 +0.8 1,173,189 + Pf6N2E2_1003 0.63 -0.3 1,173,189 + Pf6N2E2_1003 0.63 +0.8 1,173,190 - Pf6N2E2_1003 0.63 +0.8 1,173,219 + Pf6N2E2_1003 0.66 -0.2
Or see this region's nucleotide sequence