Experiment: Ying_OrganicAcid7 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_64 and Pf6N2E2_65 are separated by 138 nucleotides Pf6N2E2_65 and Pf6N2E2_66 are separated by 66 nucleotides Pf6N2E2_66 and Pf6N2E2_67 are separated by 54 nucleotides
Pf6N2E2_64 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 104,440 to 105,084
_64
Pf6N2E2_65 - SAM-dependent methyltransferase YafE (UbiE paralog), at 105,223 to 105,987
_65
Pf6N2E2_66 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 106,054 to 107,136
_66
Pf6N2E2_67 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 107,191 to 108,303
_67
Position (kb) 106
107
108 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 105.092 kb on + strand at 105.251 kb on + strand at 105.252 kb on - strand at 105.252 kb on - strand at 105.283 kb on + strand at 105.329 kb on + strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.436 kb on + strand, within Pf6N2E2_65 at 105.436 kb on + strand, within Pf6N2E2_65 at 105.437 kb on - strand, within Pf6N2E2_65 at 105.697 kb on + strand, within Pf6N2E2_65 at 105.698 kb on - strand, within Pf6N2E2_65 at 105.698 kb on - strand, within Pf6N2E2_65 at 105.801 kb on + strand, within Pf6N2E2_65 at 105.802 kb on - strand, within Pf6N2E2_65 at 105.802 kb on - strand, within Pf6N2E2_65 at 105.829 kb on + strand, within Pf6N2E2_65 at 105.829 kb on + strand, within Pf6N2E2_65 at 105.855 kb on + strand, within Pf6N2E2_65 at 105.954 kb on + strand at 105.955 kb on - strand at 106.013 kb on - strand at 106.013 kb on - strand at 106.147 kb on + strand at 106.147 kb on + strand at 106.148 kb on - strand at 106.148 kb on - strand at 106.470 kb on + strand, within Pf6N2E2_66 at 106.470 kb on + strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.529 kb on - strand, within Pf6N2E2_66 at 106.529 kb on - strand, within Pf6N2E2_66 at 106.543 kb on + strand, within Pf6N2E2_66 at 106.637 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.882 kb on + strand, within Pf6N2E2_66 at 106.882 kb on + strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.975 kb on + strand, within Pf6N2E2_66 at 107.076 kb on + strand at 107.111 kb on + strand at 107.177 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 2 remove 105,092 + -0.5 105,251 + -1.0 105,252 - -4.1 105,252 - -3.2 105,283 + +0.9 105,329 + Pf6N2E2_65 0.14 -0.5 105,330 - Pf6N2E2_65 0.14 -0.1 105,330 - Pf6N2E2_65 0.14 -2.5 105,330 - Pf6N2E2_65 0.14 -3.3 105,330 - Pf6N2E2_65 0.14 +0.9 105,330 - Pf6N2E2_65 0.14 -0.1 105,436 + Pf6N2E2_65 0.28 +0.9 105,436 + Pf6N2E2_65 0.28 -1.2 105,437 - Pf6N2E2_65 0.28 -1.5 105,697 + Pf6N2E2_65 0.62 +0.9 105,698 - Pf6N2E2_65 0.62 -0.1 105,698 - Pf6N2E2_65 0.62 +0.9 105,801 + Pf6N2E2_65 0.76 -0.5 105,802 - Pf6N2E2_65 0.76 -0.1 105,802 - Pf6N2E2_65 0.76 +0.9 105,829 + Pf6N2E2_65 0.79 -0.1 105,829 + Pf6N2E2_65 0.79 -1.3 105,855 + Pf6N2E2_65 0.83 -1.1 105,954 + -0.1 105,955 - +0.9 106,013 - +0.9 106,013 - -0.1 106,147 + +0.9 106,147 + -3.2 106,148 - +0.9 106,148 - +0.9 106,470 + Pf6N2E2_66 0.38 +0.9 106,470 + Pf6N2E2_66 0.38 +0.9 106,475 - Pf6N2E2_66 0.39 -0.1 106,475 - Pf6N2E2_66 0.39 -3.1 106,475 - Pf6N2E2_66 0.39 -0.1 106,529 - Pf6N2E2_66 0.44 +0.9 106,529 - Pf6N2E2_66 0.44 -0.1 106,543 + Pf6N2E2_66 0.45 -4.0 106,637 + Pf6N2E2_66 0.54 -0.1 106,717 + Pf6N2E2_66 0.61 -0.1 106,717 + Pf6N2E2_66 0.61 -0.1 106,717 + Pf6N2E2_66 0.61 +0.9 106,717 + Pf6N2E2_66 0.61 +0.9 106,717 + Pf6N2E2_66 0.61 +0.9 106,717 + Pf6N2E2_66 0.61 +0.9 106,718 - Pf6N2E2_66 0.61 -0.7 106,718 - Pf6N2E2_66 0.61 -7.2 106,718 - Pf6N2E2_66 0.61 -0.1 106,718 - Pf6N2E2_66 0.61 +0.9 106,718 - Pf6N2E2_66 0.61 -5.8 106,718 - Pf6N2E2_66 0.61 +0.9 106,718 - Pf6N2E2_66 0.61 -0.1 106,882 + Pf6N2E2_66 0.76 +0.9 106,882 + Pf6N2E2_66 0.76 -5.1 106,883 - Pf6N2E2_66 0.77 -2.3 106,883 - Pf6N2E2_66 0.77 +0.9 106,883 - Pf6N2E2_66 0.77 -2.3 106,883 - Pf6N2E2_66 0.77 -0.1 106,883 - Pf6N2E2_66 0.77 -1.7 106,975 + Pf6N2E2_66 0.85 -2.5 107,076 + -0.7 107,111 + -1.3 107,177 + +0.9
Or see this region's nucleotide sequence