Experiment: Ying_OrganicAcid7 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_357 and Pf6N2E2_358 are separated by 21 nucleotides Pf6N2E2_358 and Pf6N2E2_359 are separated by 36 nucleotides Pf6N2E2_359 and Pf6N2E2_360 are separated by 86 nucleotides
Pf6N2E2_357 - hypothetical protein, at 422,979 to 423,353
_357
Pf6N2E2_358 - Haloacid dehalogenase, type II, at 423,375 to 424,043
_358
Pf6N2E2_359 - Opine oxidase subunit B, at 424,080 to 425,354
_359
Pf6N2E2_360 - ABC peptide transporter, periplasmic ligand binding protein, at 425,441 to 427,084
_360
Position (kb)
424
425
426 Strain fitness (log2 ratio)
-2
-1
0
1 at 423.137 kb on + strand, within Pf6N2E2_357 at 423.137 kb on + strand, within Pf6N2E2_357 at 423.138 kb on - strand, within Pf6N2E2_357 at 423.138 kb on - strand, within Pf6N2E2_357 at 423.282 kb on + strand, within Pf6N2E2_357 at 423.282 kb on + strand, within Pf6N2E2_357 at 423.283 kb on - strand, within Pf6N2E2_357 at 423.283 kb on - strand, within Pf6N2E2_357 at 423.396 kb on + strand at 423.396 kb on + strand at 423.397 kb on - strand at 423.397 kb on - strand at 423.397 kb on - strand at 423.397 kb on - strand at 423.397 kb on - strand at 423.397 kb on - strand at 423.556 kb on - strand, within Pf6N2E2_358 at 423.633 kb on + strand, within Pf6N2E2_358 at 423.633 kb on + strand, within Pf6N2E2_358 at 423.781 kb on - strand, within Pf6N2E2_358 at 423.813 kb on + strand, within Pf6N2E2_358 at 424.095 kb on + strand at 424.360 kb on - strand, within Pf6N2E2_359 at 424.363 kb on - strand, within Pf6N2E2_359 at 424.371 kb on + strand, within Pf6N2E2_359 at 424.477 kb on - strand at 424.861 kb on + strand, within Pf6N2E2_359 at 424.862 kb on - strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.909 kb on - strand, within Pf6N2E2_359 at 424.909 kb on - strand, within Pf6N2E2_359 at 424.909 kb on - strand, within Pf6N2E2_359 at 424.909 kb on - strand, within Pf6N2E2_359 at 424.909 kb on - strand, within Pf6N2E2_359 at 424.909 kb on - strand, within Pf6N2E2_359 at 424.909 kb on - strand, within Pf6N2E2_359 at 424.935 kb on + strand, within Pf6N2E2_359 at 424.936 kb on - strand, within Pf6N2E2_359 at 425.085 kb on + strand, within Pf6N2E2_359 at 425.085 kb on + strand, within Pf6N2E2_359 at 425.325 kb on + strand at 425.328 kb on + strand at 425.344 kb on - strand at 425.414 kb on + strand at 425.432 kb on - strand at 425.735 kb on + strand, within Pf6N2E2_360 at 425.736 kb on - strand, within Pf6N2E2_360 at 425.736 kb on - strand, within Pf6N2E2_360 at 425.747 kb on + strand, within Pf6N2E2_360 at 425.747 kb on + strand, within Pf6N2E2_360 at 425.747 kb on + strand, within Pf6N2E2_360 at 425.747 kb on + strand, within Pf6N2E2_360 at 425.748 kb on - strand, within Pf6N2E2_360 at 425.748 kb on - strand, within Pf6N2E2_360 at 425.756 kb on + strand, within Pf6N2E2_360 at 425.757 kb on - strand, within Pf6N2E2_360 at 426.013 kb on - strand, within Pf6N2E2_360 at 426.013 kb on - strand, within Pf6N2E2_360 at 426.013 kb on - strand, within Pf6N2E2_360 at 426.172 kb on + strand, within Pf6N2E2_360 at 426.180 kb on - strand, within Pf6N2E2_360 at 426.180 kb on - strand, within Pf6N2E2_360 at 426.180 kb on - strand, within Pf6N2E2_360 at 426.201 kb on - strand, within Pf6N2E2_360
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 2 remove 423,137 + Pf6N2E2_357 0.42 +0.9 423,137 + Pf6N2E2_357 0.42 +0.9 423,138 - Pf6N2E2_357 0.42 +0.9 423,138 - Pf6N2E2_357 0.42 -0.7 423,282 + Pf6N2E2_357 0.81 -0.1 423,282 + Pf6N2E2_357 0.81 +0.9 423,283 - Pf6N2E2_357 0.81 -0.1 423,283 - Pf6N2E2_357 0.81 -0.7 423,396 + -0.1 423,396 + +0.9 423,397 - -0.3 423,397 - -0.6 423,397 - -0.1 423,397 - -0.1 423,397 - -0.1 423,397 - -0.4 423,556 - Pf6N2E2_358 0.27 +0.9 423,633 + Pf6N2E2_358 0.39 -0.1 423,633 + Pf6N2E2_358 0.39 -0.1 423,781 - Pf6N2E2_358 0.61 +0.9 423,813 + Pf6N2E2_358 0.65 +0.9 424,095 + -0.1 424,360 - Pf6N2E2_359 0.22 -1.7 424,363 - Pf6N2E2_359 0.22 -0.6 424,371 + Pf6N2E2_359 0.23 -0.9 424,477 - +0.9 424,861 + Pf6N2E2_359 0.61 +0.9 424,862 - Pf6N2E2_359 0.61 +0.9 424,908 + Pf6N2E2_359 0.65 -1.3 424,908 + Pf6N2E2_359 0.65 +0.9 424,908 + Pf6N2E2_359 0.65 +0.9 424,908 + Pf6N2E2_359 0.65 -1.3 424,908 + Pf6N2E2_359 0.65 -1.1 424,908 + Pf6N2E2_359 0.65 -1.2 424,908 + Pf6N2E2_359 0.65 +0.9 424,908 + Pf6N2E2_359 0.65 -1.1 424,908 + Pf6N2E2_359 0.65 +0.9 424,909 - Pf6N2E2_359 0.65 -0.1 424,909 - Pf6N2E2_359 0.65 -1.0 424,909 - Pf6N2E2_359 0.65 -1.9 424,909 - Pf6N2E2_359 0.65 -0.8 424,909 - Pf6N2E2_359 0.65 +0.9 424,909 - Pf6N2E2_359 0.65 -1.4 424,909 - Pf6N2E2_359 0.65 -0.1 424,935 + Pf6N2E2_359 0.67 +0.9 424,936 - Pf6N2E2_359 0.67 -0.1 425,085 + Pf6N2E2_359 0.79 -2.1 425,085 + Pf6N2E2_359 0.79 -0.1 425,325 + -0.9 425,328 + -1.4 425,344 - -0.1 425,414 + +0.9 425,432 - -0.7 425,735 + Pf6N2E2_360 0.18 -0.6 425,736 - Pf6N2E2_360 0.18 +0.9 425,736 - Pf6N2E2_360 0.18 +1.4 425,747 + Pf6N2E2_360 0.19 -0.1 425,747 + Pf6N2E2_360 0.19 +0.9 425,747 + Pf6N2E2_360 0.19 +0.9 425,747 + Pf6N2E2_360 0.19 +0.9 425,748 - Pf6N2E2_360 0.19 -0.1 425,748 - Pf6N2E2_360 0.19 +0.9 425,756 + Pf6N2E2_360 0.19 -0.1 425,757 - Pf6N2E2_360 0.19 -0.5 426,013 - Pf6N2E2_360 0.35 -0.7 426,013 - Pf6N2E2_360 0.35 -0.1 426,013 - Pf6N2E2_360 0.35 -0.3 426,172 + Pf6N2E2_360 0.44 -0.1 426,180 - Pf6N2E2_360 0.45 +0.3 426,180 - Pf6N2E2_360 0.45 +0.6 426,180 - Pf6N2E2_360 0.45 +0.9 426,201 - Pf6N2E2_360 0.46 +0.9
Or see this region's nucleotide sequence