Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_320

Experiment: Ying_OrganicAcid7 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_318 and Pf6N2E2_319 are separated by 206 nucleotidesPf6N2E2_319 and Pf6N2E2_320 overlap by 4 nucleotidesPf6N2E2_320 and Pf6N2E2_321 are separated by 81 nucleotides Pf6N2E2_318 - Gluconolactonase (EC 3.1.1.17), at 385,447 to 386,538 _318 Pf6N2E2_319 - DUF1850 domain-containing protein, at 386,745 to 387,131 _319 Pf6N2E2_320 - TRAP transporter, 4TM/12TM fusion protein, unknown substrate 1, at 387,128 to 389,155 _320 Pf6N2E2_321 - TRAP transporter solute receptor, unknown substrate 1, at 389,237 to 390,187 _321 Position (kb) 387 388 389 390Strain fitness (log2 ratio) -2 -1 0 1at 386.167 kb on + strand, within Pf6N2E2_318at 386.167 kb on + strand, within Pf6N2E2_318at 386.168 kb on - strand, within Pf6N2E2_318at 386.497 kb on + strandat 386.497 kb on + strandat 386.497 kb on + strandat 386.498 kb on - strandat 386.498 kb on - strandat 386.498 kb on - strandat 386.605 kb on - strandat 386.614 kb on - strandat 386.841 kb on + strand, within Pf6N2E2_319at 386.842 kb on - strand, within Pf6N2E2_319at 386.842 kb on - strand, within Pf6N2E2_319at 386.912 kb on - strand, within Pf6N2E2_319at 387.351 kb on + strand, within Pf6N2E2_320at 387.352 kb on - strand, within Pf6N2E2_320at 387.400 kb on - strand, within Pf6N2E2_320at 387.400 kb on - strand, within Pf6N2E2_320at 387.400 kb on - strand, within Pf6N2E2_320at 387.546 kb on + strand, within Pf6N2E2_320at 387.546 kb on + strand, within Pf6N2E2_320at 387.627 kb on + strand, within Pf6N2E2_320at 387.627 kb on + strand, within Pf6N2E2_320at 387.627 kb on + strand, within Pf6N2E2_320at 387.628 kb on - strand, within Pf6N2E2_320at 387.646 kb on + strand, within Pf6N2E2_320at 387.726 kb on + strand, within Pf6N2E2_320at 387.830 kb on + strand, within Pf6N2E2_320at 387.830 kb on + strand, within Pf6N2E2_320at 387.831 kb on - strand, within Pf6N2E2_320at 387.993 kb on + strand, within Pf6N2E2_320at 387.994 kb on - strand, within Pf6N2E2_320at 388.099 kb on - strand, within Pf6N2E2_320at 388.099 kb on - strand, within Pf6N2E2_320at 388.613 kb on - strand, within Pf6N2E2_320at 388.613 kb on - strand, within Pf6N2E2_320at 388.613 kb on - strand, within Pf6N2E2_320at 388.613 kb on - strand, within Pf6N2E2_320at 388.614 kb on + strand, within Pf6N2E2_320at 388.615 kb on - strand, within Pf6N2E2_320at 388.615 kb on - strand, within Pf6N2E2_320at 388.629 kb on + strand, within Pf6N2E2_320at 388.629 kb on + strand, within Pf6N2E2_320at 388.630 kb on - strand, within Pf6N2E2_320at 388.644 kb on + strand, within Pf6N2E2_320at 388.726 kb on - strand, within Pf6N2E2_320at 388.743 kb on + strand, within Pf6N2E2_320at 388.899 kb on + strand, within Pf6N2E2_320at 389.015 kb on + strandat 389.015 kb on + strandat 389.015 kb on + strandat 389.015 kb on + strandat 389.016 kb on - strandat 389.016 kb on - strandat 389.016 kb on - strandat 389.238 kb on + strandat 389.238 kb on + strandat 389.239 kb on - strandat 389.259 kb on + strandat 389.259 kb on + strandat 389.260 kb on - strandat 389.260 kb on - strandat 389.475 kb on + strand, within Pf6N2E2_321at 389.475 kb on + strand, within Pf6N2E2_321at 389.475 kb on + strand, within Pf6N2E2_321at 389.475 kb on + strand, within Pf6N2E2_321at 389.476 kb on - strand, within Pf6N2E2_321at 389.476 kb on - strand, within Pf6N2E2_321at 389.476 kb on - strand, within Pf6N2E2_321at 389.479 kb on + strand, within Pf6N2E2_321at 389.480 kb on - strand, within Pf6N2E2_321at 389.549 kb on + strand, within Pf6N2E2_321at 389.549 kb on + strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.553 kb on + strand, within Pf6N2E2_321at 389.553 kb on + strand, within Pf6N2E2_321at 389.553 kb on + strand, within Pf6N2E2_321at 389.553 kb on + strand, within Pf6N2E2_321at 389.553 kb on + strand, within Pf6N2E2_321at 389.554 kb on - strand, within Pf6N2E2_321at 389.554 kb on - strand, within Pf6N2E2_321at 389.554 kb on - strand, within Pf6N2E2_321at 389.673 kb on + strand, within Pf6N2E2_321at 389.673 kb on + strand, within Pf6N2E2_321at 389.694 kb on + strand, within Pf6N2E2_321at 389.695 kb on - strand, within Pf6N2E2_321at 389.832 kb on + strand, within Pf6N2E2_321at 389.832 kb on + strand, within Pf6N2E2_321at 390.036 kb on + strand, within Pf6N2E2_321at 390.036 kb on + strand, within Pf6N2E2_321at 390.037 kb on - strand, within Pf6N2E2_321at 390.037 kb on - strand, within Pf6N2E2_321at 390.105 kb on + strandat 390.105 kb on + strandat 390.106 kb on - strandat 390.106 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 2
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386,167 + Pf6N2E2_318 0.66 +0.9
386,167 + Pf6N2E2_318 0.66 -0.1
386,168 - Pf6N2E2_318 0.66 -0.7
386,497 + -0.1
386,497 + -0.6
386,497 + -0.3
386,498 - +0.6
386,498 - +0.9
386,498 - -0.7
386,605 - +0.9
386,614 - +0.9
386,841 + Pf6N2E2_319 0.25 -0.4
386,842 - Pf6N2E2_319 0.25 +0.9
386,842 - Pf6N2E2_319 0.25 -0.1
386,912 - Pf6N2E2_319 0.43 -0.6
387,351 + Pf6N2E2_320 0.11 +0.9
387,352 - Pf6N2E2_320 0.11 +0.9
387,400 - Pf6N2E2_320 0.13 +0.9
387,400 - Pf6N2E2_320 0.13 -0.9
387,400 - Pf6N2E2_320 0.13 +0.9
387,546 + Pf6N2E2_320 0.21 -1.4
387,546 + Pf6N2E2_320 0.21 -0.1
387,627 + Pf6N2E2_320 0.25 +0.9
387,627 + Pf6N2E2_320 0.25 -0.8
387,627 + Pf6N2E2_320 0.25 +0.9
387,628 - Pf6N2E2_320 0.25 +0.9
387,646 + Pf6N2E2_320 0.26 +0.9
387,726 + Pf6N2E2_320 0.29 +0.9
387,830 + Pf6N2E2_320 0.35 +0.9
387,830 + Pf6N2E2_320 0.35 -1.4
387,831 - Pf6N2E2_320 0.35 +0.9
387,993 + Pf6N2E2_320 0.43 -0.1
387,994 - Pf6N2E2_320 0.43 +0.9
388,099 - Pf6N2E2_320 0.48 -1.2
388,099 - Pf6N2E2_320 0.48 +0.1
388,613 - Pf6N2E2_320 0.73 +0.9
388,613 - Pf6N2E2_320 0.73 -0.1
388,613 - Pf6N2E2_320 0.73 +0.9
388,613 - Pf6N2E2_320 0.73 +0.9
388,614 + Pf6N2E2_320 0.73 +0.9
388,615 - Pf6N2E2_320 0.73 -0.8
388,615 - Pf6N2E2_320 0.73 -0.1
388,629 + Pf6N2E2_320 0.74 +0.9
388,629 + Pf6N2E2_320 0.74 +0.9
388,630 - Pf6N2E2_320 0.74 +0.9
388,644 + Pf6N2E2_320 0.75 +0.9
388,726 - Pf6N2E2_320 0.79 -1.1
388,743 + Pf6N2E2_320 0.80 -0.1
388,899 + Pf6N2E2_320 0.87 +0.9
389,015 + +0.9
389,015 + +0.9
389,015 + +0.9
389,015 + +0.9
389,016 - +0.9
389,016 - +0.9
389,016 - -0.9
389,238 + +0.9
389,238 + +0.9
389,239 - -0.7
389,259 + -0.1
389,259 + -0.1
389,260 - +0.9
389,260 - +0.9
389,475 + Pf6N2E2_321 0.25 +0.9
389,475 + Pf6N2E2_321 0.25 -2.4
389,475 + Pf6N2E2_321 0.25 -0.1
389,475 + Pf6N2E2_321 0.25 -0.1
389,476 - Pf6N2E2_321 0.25 -1.3
389,476 - Pf6N2E2_321 0.25 -0.1
389,476 - Pf6N2E2_321 0.25 +0.9
389,479 + Pf6N2E2_321 0.25 +0.9
389,480 - Pf6N2E2_321 0.26 -1.1
389,549 + Pf6N2E2_321 0.33 -0.1
389,549 + Pf6N2E2_321 0.33 -0.1
389,550 - Pf6N2E2_321 0.33 +0.9
389,550 - Pf6N2E2_321 0.33 +0.9
389,550 - Pf6N2E2_321 0.33 -0.1
389,550 - Pf6N2E2_321 0.33 -1.2
389,550 - Pf6N2E2_321 0.33 -1.4
389,550 - Pf6N2E2_321 0.33 +0.9
389,553 + Pf6N2E2_321 0.33 +0.9
389,553 + Pf6N2E2_321 0.33 +0.9
389,553 + Pf6N2E2_321 0.33 -0.1
389,553 + Pf6N2E2_321 0.33 +0.9
389,553 + Pf6N2E2_321 0.33 +0.9
389,554 - Pf6N2E2_321 0.33 -0.1
389,554 - Pf6N2E2_321 0.33 +0.9
389,554 - Pf6N2E2_321 0.33 -1.7
389,673 + Pf6N2E2_321 0.46 -0.3
389,673 + Pf6N2E2_321 0.46 +0.9
389,694 + Pf6N2E2_321 0.48 +0.9
389,695 - Pf6N2E2_321 0.48 +0.9
389,832 + Pf6N2E2_321 0.63 +0.9
389,832 + Pf6N2E2_321 0.63 +0.9
390,036 + Pf6N2E2_321 0.84 -0.4
390,036 + Pf6N2E2_321 0.84 +0.9
390,037 - Pf6N2E2_321 0.84 -0.1
390,037 - Pf6N2E2_321 0.84 +0.9
390,105 + +0.9
390,105 + +0.9
390,106 - -0.7
390,106 - +0.9

Or see this region's nucleotide sequence