Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_318

Experiment: Ying_OrganicAcid7 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_317 and Pf6N2E2_318 are separated by 96 nucleotidesPf6N2E2_318 and Pf6N2E2_319 are separated by 206 nucleotidesPf6N2E2_319 and Pf6N2E2_320 overlap by 4 nucleotides Pf6N2E2_317 - Signal transduction histidine kinase, at 384,220 to 385,350 _317 Pf6N2E2_318 - Gluconolactonase (EC 3.1.1.17), at 385,447 to 386,538 _318 Pf6N2E2_319 - DUF1850 domain-containing protein, at 386,745 to 387,131 _319 Pf6N2E2_320 - TRAP transporter, 4TM/12TM fusion protein, unknown substrate 1, at 387,128 to 389,155 _320 Position (kb) 385 386 387Strain fitness (log2 ratio) -2 -1 0 1 2at 384.540 kb on + strand, within Pf6N2E2_317at 384.540 kb on + strand, within Pf6N2E2_317at 384.540 kb on + strand, within Pf6N2E2_317at 384.540 kb on + strand, within Pf6N2E2_317at 384.541 kb on - strand, within Pf6N2E2_317at 384.646 kb on + strand, within Pf6N2E2_317at 384.675 kb on + strand, within Pf6N2E2_317at 384.676 kb on - strand, within Pf6N2E2_317at 384.799 kb on + strand, within Pf6N2E2_317at 384.799 kb on + strand, within Pf6N2E2_317at 384.921 kb on + strand, within Pf6N2E2_317at 384.922 kb on - strand, within Pf6N2E2_317at 384.922 kb on - strand, within Pf6N2E2_317at 384.922 kb on - strand, within Pf6N2E2_317at 384.943 kb on + strand, within Pf6N2E2_317at 384.943 kb on + strand, within Pf6N2E2_317at 385.022 kb on + strand, within Pf6N2E2_317at 385.176 kb on + strand, within Pf6N2E2_317at 385.176 kb on + strand, within Pf6N2E2_317at 385.176 kb on + strand, within Pf6N2E2_317at 385.210 kb on + strand, within Pf6N2E2_317at 385.210 kb on + strand, within Pf6N2E2_317at 385.262 kb on + strandat 385.329 kb on + strandat 385.404 kb on - strandat 385.427 kb on + strandat 385.428 kb on - strandat 385.428 kb on - strandat 385.428 kb on - strandat 385.605 kb on + strand, within Pf6N2E2_318at 385.911 kb on + strand, within Pf6N2E2_318at 386.030 kb on - strand, within Pf6N2E2_318at 386.071 kb on + strand, within Pf6N2E2_318at 386.167 kb on + strand, within Pf6N2E2_318at 386.167 kb on + strand, within Pf6N2E2_318at 386.168 kb on - strand, within Pf6N2E2_318at 386.497 kb on + strandat 386.497 kb on + strandat 386.497 kb on + strandat 386.498 kb on - strandat 386.498 kb on - strandat 386.498 kb on - strandat 386.605 kb on - strandat 386.614 kb on - strandat 386.841 kb on + strand, within Pf6N2E2_319at 386.842 kb on - strand, within Pf6N2E2_319at 386.842 kb on - strand, within Pf6N2E2_319at 386.912 kb on - strand, within Pf6N2E2_319at 387.351 kb on + strand, within Pf6N2E2_320at 387.352 kb on - strand, within Pf6N2E2_320at 387.400 kb on - strand, within Pf6N2E2_320at 387.400 kb on - strand, within Pf6N2E2_320at 387.400 kb on - strand, within Pf6N2E2_320

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 2
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384,540 + Pf6N2E2_317 0.28 -0.7
384,540 + Pf6N2E2_317 0.28 +0.9
384,540 + Pf6N2E2_317 0.28 +0.9
384,540 + Pf6N2E2_317 0.28 -0.9
384,541 - Pf6N2E2_317 0.28 +0.9
384,646 + Pf6N2E2_317 0.38 -0.2
384,675 + Pf6N2E2_317 0.40 +0.3
384,676 - Pf6N2E2_317 0.40 +1.9
384,799 + Pf6N2E2_317 0.51 +0.9
384,799 + Pf6N2E2_317 0.51 -0.1
384,921 + Pf6N2E2_317 0.62 -0.1
384,922 - Pf6N2E2_317 0.62 +0.9
384,922 - Pf6N2E2_317 0.62 +0.9
384,922 - Pf6N2E2_317 0.62 +0.9
384,943 + Pf6N2E2_317 0.64 +0.9
384,943 + Pf6N2E2_317 0.64 +0.9
385,022 + Pf6N2E2_317 0.71 +0.3
385,176 + Pf6N2E2_317 0.85 +0.9
385,176 + Pf6N2E2_317 0.85 -0.7
385,176 + Pf6N2E2_317 0.85 +0.9
385,210 + Pf6N2E2_317 0.88 +0.9
385,210 + Pf6N2E2_317 0.88 -1.1
385,262 + +0.9
385,329 + -0.7
385,404 - +0.9
385,427 + -0.9
385,428 - -2.0
385,428 - -0.1
385,428 - +1.5
385,605 + Pf6N2E2_318 0.14 -1.7
385,911 + Pf6N2E2_318 0.42 +0.9
386,030 - Pf6N2E2_318 0.53 +0.9
386,071 + Pf6N2E2_318 0.57 -0.1
386,167 + Pf6N2E2_318 0.66 +0.9
386,167 + Pf6N2E2_318 0.66 -0.1
386,168 - Pf6N2E2_318 0.66 -0.7
386,497 + -0.1
386,497 + -0.6
386,497 + -0.3
386,498 - +0.6
386,498 - +0.9
386,498 - -0.7
386,605 - +0.9
386,614 - +0.9
386,841 + Pf6N2E2_319 0.25 -0.4
386,842 - Pf6N2E2_319 0.25 +0.9
386,842 - Pf6N2E2_319 0.25 -0.1
386,912 - Pf6N2E2_319 0.43 -0.6
387,351 + Pf6N2E2_320 0.11 +0.9
387,352 - Pf6N2E2_320 0.11 +0.9
387,400 - Pf6N2E2_320 0.13 +0.9
387,400 - Pf6N2E2_320 0.13 -0.9
387,400 - Pf6N2E2_320 0.13 +0.9

Or see this region's nucleotide sequence