Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2299

Experiment: Ying_OrganicAcid7 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2298 and Pf6N2E2_2299 overlap by 4 nucleotidesPf6N2E2_2299 and Pf6N2E2_2300 are separated by 187 nucleotides Pf6N2E2_2298 - Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps, at 2,721,089 to 2,722,423 _2298 Pf6N2E2_2299 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusA, at 2,722,420 to 2,722,671 _2299 Pf6N2E2_2300 - LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM, at 2,722,859 to 2,723,932 _2300 Position (kb) 2722 2723Strain fitness (log2 ratio) -2 -1 0 1at 2721.443 kb on + strand, within Pf6N2E2_2298at 2721.443 kb on + strand, within Pf6N2E2_2298at 2721.444 kb on - strand, within Pf6N2E2_2298at 2721.444 kb on - strand, within Pf6N2E2_2298at 2721.485 kb on + strand, within Pf6N2E2_2298at 2721.485 kb on + strand, within Pf6N2E2_2298at 2721.486 kb on - strand, within Pf6N2E2_2298at 2721.492 kb on + strand, within Pf6N2E2_2298at 2721.492 kb on + strand, within Pf6N2E2_2298at 2721.493 kb on - strand, within Pf6N2E2_2298at 2721.493 kb on - strand, within Pf6N2E2_2298at 2721.581 kb on + strand, within Pf6N2E2_2298at 2721.581 kb on + strand, within Pf6N2E2_2298at 2721.581 kb on + strand, within Pf6N2E2_2298at 2721.581 kb on + strand, within Pf6N2E2_2298at 2721.581 kb on + strand, within Pf6N2E2_2298at 2721.581 kb on + strand, within Pf6N2E2_2298at 2721.581 kb on + strand, within Pf6N2E2_2298at 2721.582 kb on - strand, within Pf6N2E2_2298at 2721.582 kb on - strand, within Pf6N2E2_2298at 2721.582 kb on - strand, within Pf6N2E2_2298at 2721.582 kb on - strand, within Pf6N2E2_2298at 2721.582 kb on - strand, within Pf6N2E2_2298at 2721.593 kb on + strand, within Pf6N2E2_2298at 2721.593 kb on + strand, within Pf6N2E2_2298at 2721.593 kb on + strand, within Pf6N2E2_2298at 2721.593 kb on + strand, within Pf6N2E2_2298at 2721.594 kb on - strand, within Pf6N2E2_2298at 2721.708 kb on - strand, within Pf6N2E2_2298at 2721.708 kb on - strand, within Pf6N2E2_2298at 2721.708 kb on - strand, within Pf6N2E2_2298at 2721.920 kb on + strand, within Pf6N2E2_2298at 2722.029 kb on - strand, within Pf6N2E2_2298at 2722.085 kb on + strand, within Pf6N2E2_2298at 2722.085 kb on + strand, within Pf6N2E2_2298at 2722.085 kb on + strand, within Pf6N2E2_2298at 2722.086 kb on - strand, within Pf6N2E2_2298at 2722.086 kb on - strand, within Pf6N2E2_2298at 2722.086 kb on - strand, within Pf6N2E2_2298at 2722.086 kb on - strand, within Pf6N2E2_2298at 2722.086 kb on - strand, within Pf6N2E2_2298at 2722.134 kb on - strand, within Pf6N2E2_2298at 2722.677 kb on - strandat 2722.677 kb on - strandat 2722.926 kb on + strandat 2722.927 kb on - strandat 2722.927 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 2
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2,721,443 + Pf6N2E2_2298 0.27 +0.9
2,721,443 + Pf6N2E2_2298 0.27 +0.5
2,721,444 - Pf6N2E2_2298 0.27 -0.1
2,721,444 - Pf6N2E2_2298 0.27 +0.9
2,721,485 + Pf6N2E2_2298 0.30 -0.1
2,721,485 + Pf6N2E2_2298 0.30 +0.9
2,721,486 - Pf6N2E2_2298 0.30 -0.1
2,721,492 + Pf6N2E2_2298 0.30 -0.1
2,721,492 + Pf6N2E2_2298 0.30 -2.6
2,721,493 - Pf6N2E2_2298 0.30 -0.1
2,721,493 - Pf6N2E2_2298 0.30 -0.7
2,721,581 + Pf6N2E2_2298 0.37 +0.9
2,721,581 + Pf6N2E2_2298 0.37 +0.9
2,721,581 + Pf6N2E2_2298 0.37 -0.1
2,721,581 + Pf6N2E2_2298 0.37 -0.7
2,721,581 + Pf6N2E2_2298 0.37 -0.1
2,721,581 + Pf6N2E2_2298 0.37 -0.6
2,721,581 + Pf6N2E2_2298 0.37 -0.5
2,721,582 - Pf6N2E2_2298 0.37 +0.9
2,721,582 - Pf6N2E2_2298 0.37 +0.9
2,721,582 - Pf6N2E2_2298 0.37 +0.9
2,721,582 - Pf6N2E2_2298 0.37 -0.1
2,721,582 - Pf6N2E2_2298 0.37 -0.1
2,721,593 + Pf6N2E2_2298 0.38 -0.7
2,721,593 + Pf6N2E2_2298 0.38 -0.9
2,721,593 + Pf6N2E2_2298 0.38 -0.6
2,721,593 + Pf6N2E2_2298 0.38 +0.9
2,721,594 - Pf6N2E2_2298 0.38 +0.9
2,721,708 - Pf6N2E2_2298 0.46 -0.9
2,721,708 - Pf6N2E2_2298 0.46 +0.9
2,721,708 - Pf6N2E2_2298 0.46 -0.1
2,721,920 + Pf6N2E2_2298 0.62 -0.1
2,722,029 - Pf6N2E2_2298 0.70 -0.3
2,722,085 + Pf6N2E2_2298 0.75 -0.1
2,722,085 + Pf6N2E2_2298 0.75 -0.5
2,722,085 + Pf6N2E2_2298 0.75 -0.7
2,722,086 - Pf6N2E2_2298 0.75 +0.9
2,722,086 - Pf6N2E2_2298 0.75 +0.9
2,722,086 - Pf6N2E2_2298 0.75 -1.1
2,722,086 - Pf6N2E2_2298 0.75 +0.3
2,722,086 - Pf6N2E2_2298 0.75 -0.1
2,722,134 - Pf6N2E2_2298 0.78 +0.9
2,722,677 - -0.1
2,722,677 - -0.1
2,722,926 + +0.9
2,722,927 - -0.7
2,722,927 - +0.9

Or see this region's nucleotide sequence