Experiment: Ying_AminoAcid20 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_95 and Pf6N2E2_96 are separated by 4 nucleotides Pf6N2E2_96 and Pf6N2E2_97 are separated by 93 nucleotides Pf6N2E2_97 and Pf6N2E2_98 overlap by 4 nucleotides Pf6N2E2_98 and Pf6N2E2_99 are separated by 53 nucleotides Pf6N2E2_99 and Pf6N2E2_100 are separated by 298 nucleotides
Pf6N2E2_95 - putative oxygenase (putative secreted protein), at 140,697 to 141,782
_95
Pf6N2E2_96 - FIG011065: hypothetical protein, at 141,787 to 142,551
_96
Pf6N2E2_97 - putataive branched-chain amino acid transport protein AzlC, at 142,645 to 143,355
_97
Pf6N2E2_98 - putative transmembrane protein, at 143,352 to 143,660
_98
Pf6N2E2_99 - hypothetical protein, at 143,714 to 143,854
_99
Pf6N2E2_100 - membrane protein, putative, at 144,153 to 145,364
_100
Position (kb) 142
143
144 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 141.806 kb on + strand at 141.806 kb on + strand at 141.806 kb on + strand at 141.806 kb on + strand at 141.806 kb on + strand at 141.806 kb on + strand at 141.806 kb on + strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 142.107 kb on - strand, within Pf6N2E2_96 at 142.107 kb on - strand, within Pf6N2E2_96 at 142.126 kb on + strand, within Pf6N2E2_96 at 142.127 kb on - strand, within Pf6N2E2_96 at 142.229 kb on - strand, within Pf6N2E2_96 at 142.390 kb on + strand, within Pf6N2E2_96 at 142.390 kb on + strand, within Pf6N2E2_96 at 142.390 kb on + strand, within Pf6N2E2_96 at 142.391 kb on - strand, within Pf6N2E2_96 at 142.528 kb on + strand at 142.528 kb on + strand at 142.528 kb on + strand at 142.528 kb on + strand at 142.529 kb on - strand at 142.560 kb on + strand at 142.602 kb on - strand at 142.602 kb on - strand at 142.602 kb on - strand at 142.621 kb on + strand at 142.621 kb on + strand at 142.621 kb on + strand at 142.621 kb on + strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.694 kb on - strand at 142.694 kb on - strand at 142.694 kb on - strand at 142.694 kb on - strand at 142.788 kb on + strand, within Pf6N2E2_97 at 142.788 kb on + strand, within Pf6N2E2_97 at 142.788 kb on + strand, within Pf6N2E2_97 at 142.788 kb on + strand, within Pf6N2E2_97 at 142.789 kb on - strand, within Pf6N2E2_97 at 142.800 kb on - strand, within Pf6N2E2_97 at 143.230 kb on - strand, within Pf6N2E2_97 at 143.392 kb on + strand, within Pf6N2E2_98 at 143.393 kb on - strand, within Pf6N2E2_98 at 143.629 kb on + strand, within Pf6N2E2_98 at 143.630 kb on - strand at 143.757 kb on - strand, within Pf6N2E2_99 at 143.757 kb on - strand, within Pf6N2E2_99 at 143.757 kb on - strand, within Pf6N2E2_99 at 143.768 kb on - strand, within Pf6N2E2_99 at 143.870 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.924 kb on - strand at 143.924 kb on - strand at 143.954 kb on - strand at 143.954 kb on - strand at 143.976 kb on - strand at 144.117 kb on + strand at 144.117 kb on + strand at 144.118 kb on - strand at 144.118 kb on - strand at 144.118 kb on - strand at 144.118 kb on - strand at 144.128 kb on + strand at 144.129 kb on - strand at 144.129 kb on - strand at 144.129 kb on - strand at 144.129 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.172 kb on + strand at 144.172 kb on + strand at 144.172 kb on + strand at 144.173 kb on - strand at 144.173 kb on - strand at 144.196 kb on + strand at 144.196 kb on + strand at 144.196 kb on + strand at 144.197 kb on - strand at 144.197 kb on - strand at 144.197 kb on - strand at 144.276 kb on - strand, within Pf6N2E2_100 at 144.300 kb on + strand, within Pf6N2E2_100
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2 remove 141,806 + -0.7 141,806 + -0.1 141,806 + -0.7 141,806 + +1.4 141,806 + -0.1 141,806 + +0.9 141,806 + -0.7 141,807 - -0.1 141,807 - -0.1 141,807 - +0.4 141,807 - -0.7 141,807 - +0.9 141,807 - -0.7 141,807 - +0.9 141,807 - -1.6 141,807 - -0.1 142,107 - Pf6N2E2_96 0.42 -0.9 142,107 - Pf6N2E2_96 0.42 +0.9 142,126 + Pf6N2E2_96 0.44 +0.2 142,127 - Pf6N2E2_96 0.44 -0.6 142,229 - Pf6N2E2_96 0.58 +0.3 142,390 + Pf6N2E2_96 0.79 +0.9 142,390 + Pf6N2E2_96 0.79 -0.1 142,390 + Pf6N2E2_96 0.79 +0.9 142,391 - Pf6N2E2_96 0.79 -0.1 142,528 + +0.9 142,528 + +0.9 142,528 + +0.9 142,528 + -0.7 142,529 - +0.9 142,560 + +0.9 142,602 - +0.9 142,602 - +0.9 142,602 - +0.9 142,621 + +0.9 142,621 + +0.9 142,621 + +0.9 142,621 + -0.7 142,622 - +0.9 142,622 - +0.9 142,622 - -0.1 142,622 - +0.6 142,622 - +0.9 142,622 - -0.1 142,622 - +0.9 142,693 + +0.9 142,693 + +0.6 142,693 + +0.9 142,693 + -0.7 142,693 + +0.9 142,693 + +0.9 142,694 - +0.9 142,694 - -0.7 142,694 - -0.7 142,694 - +0.9 142,788 + Pf6N2E2_97 0.20 +0.9 142,788 + Pf6N2E2_97 0.20 +0.9 142,788 + Pf6N2E2_97 0.20 -2.8 142,788 + Pf6N2E2_97 0.20 -2.0 142,789 - Pf6N2E2_97 0.20 -0.1 142,800 - Pf6N2E2_97 0.22 +0.9 143,230 - Pf6N2E2_97 0.82 +0.9 143,392 + Pf6N2E2_98 0.13 +0.9 143,393 - Pf6N2E2_98 0.13 +0.9 143,629 + Pf6N2E2_98 0.90 +0.9 143,630 - +0.9 143,757 - Pf6N2E2_99 0.30 -0.1 143,757 - Pf6N2E2_99 0.30 -0.1 143,757 - Pf6N2E2_99 0.30 -0.8 143,768 - Pf6N2E2_99 0.38 -0.1 143,870 + -0.1 143,923 + +0.9 143,923 + -0.2 143,923 + +0.9 143,923 + +0.9 143,923 + +0.9 143,923 + -0.1 143,924 - +0.9 143,924 - -1.4 143,954 - -0.1 143,954 - +0.9 143,976 - +0.9 144,117 + -0.1 144,117 + +0.9 144,118 - +0.9 144,118 - -0.1 144,118 - +0.9 144,118 - -0.1 144,128 + -0.1 144,129 - +0.9 144,129 - +0.9 144,129 - +0.1 144,129 - +0.9 144,132 - -0.1 144,132 - -1.4 144,132 - +0.9 144,132 - -0.1 144,132 - -0.4 144,172 + +0.9 144,172 + +0.9 144,172 + +0.9 144,173 - +0.9 144,173 - -0.1 144,196 + +0.3 144,196 + +0.9 144,196 + -0.9 144,197 - +0.9 144,197 - +0.1 144,197 - -0.1 144,276 - Pf6N2E2_100 0.10 -1.1 144,300 + Pf6N2E2_100 0.12 -1.1
Or see this region's nucleotide sequence