Experiment: Ying_AminoAcid20 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5309 and Pf6N2E2_5310 are separated by 180 nucleotides Pf6N2E2_5310 and Pf6N2E2_5311 are separated by 115 nucleotides Pf6N2E2_5311 and Pf6N2E2_5312 are separated by 160 nucleotides
Pf6N2E2_5309 - Permease of the drug/metabolite transporter (DMT) superfamily, at 6,103,293 to 6,104,195
_5309
Pf6N2E2_5310 - Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-), at 6,104,376 to 6,105,347
_5310
Pf6N2E2_5311 - FIG00959158: hypothetical protein, at 6,105,463 to 6,106,131
_5311
Pf6N2E2_5312 - FIG00964214: hypothetical protein, at 6,106,292 to 6,106,621
_5312
Position (kb)
6104
6105
6106 Strain fitness (log2 ratio)
-2
-1
0
1 at 6103.518 kb on - strand, within Pf6N2E2_5309 at 6103.518 kb on - strand, within Pf6N2E2_5309 at 6103.854 kb on + strand, within Pf6N2E2_5309 at 6103.855 kb on - strand, within Pf6N2E2_5309 at 6103.927 kb on - strand, within Pf6N2E2_5309 at 6103.944 kb on + strand, within Pf6N2E2_5309 at 6103.944 kb on + strand, within Pf6N2E2_5309 at 6103.944 kb on + strand, within Pf6N2E2_5309 at 6103.945 kb on - strand, within Pf6N2E2_5309 at 6104.025 kb on + strand, within Pf6N2E2_5309 at 6104.290 kb on + strand at 6104.291 kb on - strand at 6104.291 kb on - strand at 6104.377 kb on + strand at 6104.377 kb on + strand at 6104.377 kb on + strand at 6104.377 kb on + strand at 6104.378 kb on - strand at 6104.378 kb on - strand at 6104.378 kb on - strand at 6104.560 kb on + strand, within Pf6N2E2_5310 at 6104.560 kb on + strand, within Pf6N2E2_5310 at 6104.560 kb on + strand, within Pf6N2E2_5310 at 6104.561 kb on - strand, within Pf6N2E2_5310 at 6104.561 kb on - strand, within Pf6N2E2_5310 at 6104.827 kb on + strand, within Pf6N2E2_5310 at 6104.827 kb on + strand, within Pf6N2E2_5310 at 6104.919 kb on + strand, within Pf6N2E2_5310 at 6104.919 kb on + strand, within Pf6N2E2_5310 at 6104.919 kb on + strand, within Pf6N2E2_5310 at 6104.919 kb on + strand, within Pf6N2E2_5310 at 6104.920 kb on - strand, within Pf6N2E2_5310 at 6104.920 kb on - strand, within Pf6N2E2_5310 at 6104.920 kb on - strand, within Pf6N2E2_5310 at 6104.920 kb on - strand, within Pf6N2E2_5310 at 6104.920 kb on - strand, within Pf6N2E2_5310 at 6104.920 kb on - strand, within Pf6N2E2_5310 at 6105.054 kb on + strand, within Pf6N2E2_5310 at 6105.054 kb on + strand, within Pf6N2E2_5310 at 6105.055 kb on - strand, within Pf6N2E2_5310 at 6105.055 kb on - strand, within Pf6N2E2_5310 at 6105.055 kb on - strand, within Pf6N2E2_5310 at 6105.055 kb on - strand, within Pf6N2E2_5310 at 6105.055 kb on - strand, within Pf6N2E2_5310 at 6105.097 kb on + strand, within Pf6N2E2_5310 at 6105.097 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.109 kb on + strand, within Pf6N2E2_5310 at 6105.110 kb on - strand, within Pf6N2E2_5310 at 6105.110 kb on - strand, within Pf6N2E2_5310 at 6105.110 kb on - strand, within Pf6N2E2_5310 at 6105.110 kb on - strand, within Pf6N2E2_5310 at 6105.110 kb on - strand, within Pf6N2E2_5310 at 6105.110 kb on - strand, within Pf6N2E2_5310 at 6105.110 kb on - strand, within Pf6N2E2_5310 at 6105.318 kb on - strand at 6105.333 kb on + strand at 6105.333 kb on + strand at 6105.334 kb on - strand at 6105.334 kb on - strand at 6105.334 kb on - strand at 6105.385 kb on - strand at 6105.422 kb on + strand at 6105.423 kb on - strand at 6105.491 kb on + strand at 6105.491 kb on + strand at 6105.491 kb on + strand at 6105.653 kb on + strand, within Pf6N2E2_5311 at 6105.653 kb on + strand, within Pf6N2E2_5311 at 6105.653 kb on + strand, within Pf6N2E2_5311 at 6105.654 kb on - strand, within Pf6N2E2_5311 at 6105.654 kb on - strand, within Pf6N2E2_5311 at 6105.654 kb on - strand, within Pf6N2E2_5311 at 6105.802 kb on + strand, within Pf6N2E2_5311 at 6105.802 kb on + strand, within Pf6N2E2_5311 at 6105.802 kb on + strand, within Pf6N2E2_5311 at 6105.802 kb on + strand, within Pf6N2E2_5311 at 6105.802 kb on + strand, within Pf6N2E2_5311 at 6105.803 kb on - strand, within Pf6N2E2_5311 at 6105.803 kb on - strand, within Pf6N2E2_5311 at 6105.803 kb on - strand, within Pf6N2E2_5311 at 6105.890 kb on + strand, within Pf6N2E2_5311 at 6105.890 kb on + strand, within Pf6N2E2_5311 at 6105.891 kb on - strand, within Pf6N2E2_5311 at 6105.891 kb on - strand, within Pf6N2E2_5311 at 6105.891 kb on - strand, within Pf6N2E2_5311 at 6105.891 kb on - strand, within Pf6N2E2_5311 at 6105.920 kb on + strand, within Pf6N2E2_5311 at 6105.920 kb on + strand, within Pf6N2E2_5311 at 6105.920 kb on + strand, within Pf6N2E2_5311 at 6105.920 kb on + strand, within Pf6N2E2_5311 at 6105.921 kb on - strand, within Pf6N2E2_5311 at 6105.921 kb on - strand, within Pf6N2E2_5311 at 6105.969 kb on + strand, within Pf6N2E2_5311 at 6105.970 kb on - strand, within Pf6N2E2_5311 at 6105.971 kb on + strand, within Pf6N2E2_5311 at 6105.971 kb on + strand, within Pf6N2E2_5311 at 6105.971 kb on + strand, within Pf6N2E2_5311 at 6105.971 kb on + strand, within Pf6N2E2_5311 at 6105.971 kb on + strand, within Pf6N2E2_5311 at 6105.971 kb on + strand, within Pf6N2E2_5311 at 6105.971 kb on + strand, within Pf6N2E2_5311 at 6105.971 kb on + strand, within Pf6N2E2_5311 at 6105.972 kb on - strand, within Pf6N2E2_5311 at 6105.972 kb on - strand, within Pf6N2E2_5311 at 6105.972 kb on - strand, within Pf6N2E2_5311 at 6106.190 kb on - strand at 6106.190 kb on - strand at 6106.230 kb on + strand at 6106.231 kb on - strand at 6106.231 kb on - strand at 6106.231 kb on - strand at 6106.282 kb on + strand at 6106.282 kb on + strand at 6106.282 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2 remove 6,103,518 - Pf6N2E2_5309 0.25 +1.0 6,103,518 - Pf6N2E2_5309 0.25 -2.0 6,103,854 + Pf6N2E2_5309 0.62 -0.3 6,103,855 - Pf6N2E2_5309 0.62 -0.6 6,103,927 - Pf6N2E2_5309 0.70 +1.0 6,103,944 + Pf6N2E2_5309 0.72 +0.0 6,103,944 + Pf6N2E2_5309 0.72 +1.0 6,103,944 + Pf6N2E2_5309 0.72 +1.0 6,103,945 - Pf6N2E2_5309 0.72 +1.0 6,104,025 + Pf6N2E2_5309 0.81 +0.0 6,104,290 + +1.0 6,104,291 - +1.0 6,104,291 - +0.0 6,104,377 + +1.0 6,104,377 + -0.2 6,104,377 + +1.0 6,104,377 + -0.6 6,104,378 - -0.2 6,104,378 - -0.3 6,104,378 - -0.6 6,104,560 + Pf6N2E2_5310 0.19 +0.0 6,104,560 + Pf6N2E2_5310 0.19 +1.0 6,104,560 + Pf6N2E2_5310 0.19 +1.0 6,104,561 - Pf6N2E2_5310 0.19 +1.0 6,104,561 - Pf6N2E2_5310 0.19 +0.0 6,104,827 + Pf6N2E2_5310 0.46 -1.3 6,104,827 + Pf6N2E2_5310 0.46 -1.3 6,104,919 + Pf6N2E2_5310 0.56 +0.0 6,104,919 + Pf6N2E2_5310 0.56 +0.2 6,104,919 + Pf6N2E2_5310 0.56 -0.6 6,104,919 + Pf6N2E2_5310 0.56 +1.0 6,104,920 - Pf6N2E2_5310 0.56 -0.6 6,104,920 - Pf6N2E2_5310 0.56 -0.6 6,104,920 - Pf6N2E2_5310 0.56 -0.6 6,104,920 - Pf6N2E2_5310 0.56 +1.0 6,104,920 - Pf6N2E2_5310 0.56 -1.3 6,104,920 - Pf6N2E2_5310 0.56 -0.6 6,105,054 + Pf6N2E2_5310 0.70 +0.7 6,105,054 + Pf6N2E2_5310 0.70 -1.2 6,105,055 - Pf6N2E2_5310 0.70 +0.0 6,105,055 - Pf6N2E2_5310 0.70 +1.0 6,105,055 - Pf6N2E2_5310 0.70 -0.6 6,105,055 - Pf6N2E2_5310 0.70 +0.0 6,105,055 - Pf6N2E2_5310 0.70 +0.0 6,105,097 + Pf6N2E2_5310 0.74 +1.0 6,105,097 + Pf6N2E2_5310 0.74 +1.0 6,105,109 + Pf6N2E2_5310 0.75 +0.0 6,105,109 + Pf6N2E2_5310 0.75 -1.0 6,105,109 + Pf6N2E2_5310 0.75 -0.8 6,105,109 + Pf6N2E2_5310 0.75 +0.0 6,105,109 + Pf6N2E2_5310 0.75 +0.0 6,105,109 + Pf6N2E2_5310 0.75 -1.6 6,105,109 + Pf6N2E2_5310 0.75 -0.4 6,105,109 + Pf6N2E2_5310 0.75 +0.0 6,105,109 + Pf6N2E2_5310 0.75 +0.0 6,105,109 + Pf6N2E2_5310 0.75 +1.0 6,105,110 - Pf6N2E2_5310 0.76 +1.0 6,105,110 - Pf6N2E2_5310 0.76 +0.0 6,105,110 - Pf6N2E2_5310 0.76 +0.0 6,105,110 - Pf6N2E2_5310 0.76 -1.6 6,105,110 - Pf6N2E2_5310 0.76 +1.0 6,105,110 - Pf6N2E2_5310 0.76 -0.1 6,105,110 - Pf6N2E2_5310 0.76 +0.0 6,105,318 - +1.0 6,105,333 + +0.3 6,105,333 + -0.8 6,105,334 - +0.0 6,105,334 - +1.0 6,105,334 - -0.6 6,105,385 - +1.0 6,105,422 + -0.1 6,105,423 - +0.0 6,105,491 + +0.0 6,105,491 + -0.6 6,105,491 + +1.0 6,105,653 + Pf6N2E2_5311 0.28 +0.0 6,105,653 + Pf6N2E2_5311 0.28 +0.0 6,105,653 + Pf6N2E2_5311 0.28 +0.4 6,105,654 - Pf6N2E2_5311 0.29 -0.6 6,105,654 - Pf6N2E2_5311 0.29 +1.0 6,105,654 - Pf6N2E2_5311 0.29 +1.0 6,105,802 + Pf6N2E2_5311 0.51 +1.0 6,105,802 + Pf6N2E2_5311 0.51 -0.8 6,105,802 + Pf6N2E2_5311 0.51 +1.0 6,105,802 + Pf6N2E2_5311 0.51 +0.0 6,105,802 + Pf6N2E2_5311 0.51 -0.5 6,105,803 - Pf6N2E2_5311 0.51 -0.6 6,105,803 - Pf6N2E2_5311 0.51 +0.0 6,105,803 - Pf6N2E2_5311 0.51 +0.0 6,105,890 + Pf6N2E2_5311 0.64 +1.0 6,105,890 + Pf6N2E2_5311 0.64 -0.6 6,105,891 - Pf6N2E2_5311 0.64 -1.2 6,105,891 - Pf6N2E2_5311 0.64 -0.7 6,105,891 - Pf6N2E2_5311 0.64 +0.0 6,105,891 - Pf6N2E2_5311 0.64 -1.1 6,105,920 + Pf6N2E2_5311 0.68 +0.0 6,105,920 + Pf6N2E2_5311 0.68 +1.0 6,105,920 + Pf6N2E2_5311 0.68 +1.0 6,105,920 + Pf6N2E2_5311 0.68 -0.4 6,105,921 - Pf6N2E2_5311 0.68 -0.6 6,105,921 - Pf6N2E2_5311 0.68 +1.0 6,105,969 + Pf6N2E2_5311 0.76 -1.4 6,105,970 - Pf6N2E2_5311 0.76 +1.0 6,105,971 + Pf6N2E2_5311 0.76 -0.6 6,105,971 + Pf6N2E2_5311 0.76 -0.2 6,105,971 + Pf6N2E2_5311 0.76 -0.3 6,105,971 + Pf6N2E2_5311 0.76 +1.0 6,105,971 + Pf6N2E2_5311 0.76 -0.4 6,105,971 + Pf6N2E2_5311 0.76 -0.6 6,105,971 + Pf6N2E2_5311 0.76 -0.6 6,105,971 + Pf6N2E2_5311 0.76 +1.6 6,105,972 - Pf6N2E2_5311 0.76 +1.0 6,105,972 - Pf6N2E2_5311 0.76 +1.0 6,105,972 - Pf6N2E2_5311 0.76 +1.0 6,106,190 - +0.0 6,106,190 - +1.0 6,106,230 + -0.3 6,106,231 - -0.9 6,106,231 - +1.0 6,106,231 - +1.0 6,106,282 + -1.5 6,106,282 + -0.3 6,106,282 + -0.6
Or see this region's nucleotide sequence