Experiment: Ying_AminoAcid20 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_498 and Pf6N2E2_499 are separated by 608 nucleotides Pf6N2E2_499 and Pf6N2E2_500 are separated by 78 nucleotides
Pf6N2E2_498 - Dihydropyrimidinase (EC 3.5.2.2) <small>(from data)</small>, at 565,573 to 567,012
_498
Pf6N2E2_499 - Possible pyrimidine permease in reductive pathway, at 567,621 to 569,099
_499
Pf6N2E2_500 - Beta-ureidopropionase (EC 3.5.1.6), at 569,178 to 570,461
_500
Position (kb) 567
568
569
570 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 566.829 kb on + strand, within Pf6N2E2_498 at 566.829 kb on + strand, within Pf6N2E2_498 at 566.829 kb on + strand, within Pf6N2E2_498 at 566.830 kb on - strand, within Pf6N2E2_498 at 566.830 kb on - strand, within Pf6N2E2_498 at 566.864 kb on + strand, within Pf6N2E2_498 at 566.864 kb on + strand, within Pf6N2E2_498 at 566.864 kb on + strand, within Pf6N2E2_498 at 566.865 kb on - strand, within Pf6N2E2_498 at 566.979 kb on + strand at 567.061 kb on + strand at 567.062 kb on - strand at 567.077 kb on + strand at 567.095 kb on + strand at 567.096 kb on - strand at 567.181 kb on - strand at 567.288 kb on + strand at 567.392 kb on - strand at 567.406 kb on + strand at 567.473 kb on + strand at 567.473 kb on + strand at 567.475 kb on + strand at 567.567 kb on + strand at 567.567 kb on + strand at 567.701 kb on + strand at 567.701 kb on + strand at 567.701 kb on + strand at 567.702 kb on - strand at 567.798 kb on + strand, within Pf6N2E2_499 at 567.798 kb on + strand, within Pf6N2E2_499 at 567.799 kb on - strand, within Pf6N2E2_499 at 567.799 kb on - strand, within Pf6N2E2_499 at 568.317 kb on + strand, within Pf6N2E2_499 at 568.317 kb on + strand, within Pf6N2E2_499 at 568.318 kb on - strand, within Pf6N2E2_499 at 568.318 kb on - strand, within Pf6N2E2_499 at 568.318 kb on - strand, within Pf6N2E2_499 at 568.783 kb on - strand, within Pf6N2E2_499 at 568.848 kb on + strand, within Pf6N2E2_499 at 568.849 kb on - strand, within Pf6N2E2_499 at 568.905 kb on + strand, within Pf6N2E2_499 at 568.998 kb on + strand at 568.998 kb on + strand at 568.999 kb on - strand at 569.043 kb on + strand at 569.200 kb on + strand at 569.200 kb on + strand at 569.201 kb on - strand at 569.760 kb on + strand, within Pf6N2E2_500 at 569.761 kb on - strand, within Pf6N2E2_500 at 569.909 kb on + strand, within Pf6N2E2_500 at 569.910 kb on - strand, within Pf6N2E2_500
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2 remove 566,829 + Pf6N2E2_498 0.87 +0.9 566,829 + Pf6N2E2_498 0.87 -0.4 566,829 + Pf6N2E2_498 0.87 -0.6 566,830 - Pf6N2E2_498 0.87 +0.9 566,830 - Pf6N2E2_498 0.87 -1.1 566,864 + Pf6N2E2_498 0.90 -0.1 566,864 + Pf6N2E2_498 0.90 +0.9 566,864 + Pf6N2E2_498 0.90 +0.9 566,865 - Pf6N2E2_498 0.90 -0.7 566,979 + -0.1 567,061 + +0.9 567,062 - +0.9 567,077 + -0.1 567,095 + +0.9 567,096 - +0.9 567,181 - +0.9 567,288 + +0.9 567,392 - -0.4 567,406 + -1.1 567,473 + -0.1 567,473 + -1.7 567,475 + -0.6 567,567 + +0.9 567,567 + +0.9 567,701 + +0.9 567,701 + +0.9 567,701 + -0.1 567,702 - +0.9 567,798 + Pf6N2E2_499 0.12 +0.9 567,798 + Pf6N2E2_499 0.12 +0.9 567,799 - Pf6N2E2_499 0.12 -3.2 567,799 - Pf6N2E2_499 0.12 +0.9 568,317 + Pf6N2E2_499 0.47 -0.1 568,317 + Pf6N2E2_499 0.47 +0.9 568,318 - Pf6N2E2_499 0.47 -0.7 568,318 - Pf6N2E2_499 0.47 -0.1 568,318 - Pf6N2E2_499 0.47 +0.9 568,783 - Pf6N2E2_499 0.79 -0.1 568,848 + Pf6N2E2_499 0.83 -0.7 568,849 - Pf6N2E2_499 0.83 -0.1 568,905 + Pf6N2E2_499 0.87 +0.9 568,998 + +0.9 568,998 + +0.9 568,999 - -0.1 569,043 + -0.4 569,200 + -1.1 569,200 + -0.7 569,201 - -0.5 569,760 + Pf6N2E2_500 0.45 -0.1 569,761 - Pf6N2E2_500 0.45 -0.1 569,909 + Pf6N2E2_500 0.57 -1.3 569,910 - Pf6N2E2_500 0.57 -0.1
Or see this region's nucleotide sequence