Experiment: Ying_AminoAcid20 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4575 and Pf6N2E2_4576 are separated by 160 nucleotides Pf6N2E2_4576 and Pf6N2E2_4577 are separated by 34 nucleotides
Pf6N2E2_4575 - Coniferyl aldehyde dehydrogenase (EC 1.2.1.68), at 5,305,200 to 5,306,630
_4575
Pf6N2E2_4576 - Transcriptional regulator, TetR family, at 5,306,791 to 5,307,450
_4576
Pf6N2E2_4577 - Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1), at 5,307,485 to 5,308,339
_4577
Position (kb) 5306
5307
5308 Strain fitness (log2 ratio) -1
0
1
2 at 5305.840 kb on + strand, within Pf6N2E2_4575 at 5305.840 kb on + strand, within Pf6N2E2_4575 at 5305.841 kb on - strand, within Pf6N2E2_4575 at 5305.841 kb on - strand, within Pf6N2E2_4575 at 5306.209 kb on - strand, within Pf6N2E2_4575 at 5306.239 kb on + strand, within Pf6N2E2_4575 at 5306.239 kb on + strand, within Pf6N2E2_4575 at 5306.239 kb on + strand, within Pf6N2E2_4575 at 5306.240 kb on - strand, within Pf6N2E2_4575 at 5306.240 kb on - strand, within Pf6N2E2_4575 at 5306.240 kb on - strand, within Pf6N2E2_4575 at 5306.281 kb on + strand, within Pf6N2E2_4575 at 5306.281 kb on + strand, within Pf6N2E2_4575 at 5306.281 kb on + strand, within Pf6N2E2_4575 at 5306.282 kb on - strand, within Pf6N2E2_4575 at 5306.369 kb on - strand, within Pf6N2E2_4575 at 5306.481 kb on + strand, within Pf6N2E2_4575 at 5306.482 kb on - strand, within Pf6N2E2_4575 at 5306.482 kb on - strand, within Pf6N2E2_4575 at 5306.546 kb on - strand at 5306.611 kb on + strand at 5306.611 kb on + strand at 5306.611 kb on + strand at 5306.645 kb on + strand at 5306.646 kb on - strand at 5306.648 kb on + strand at 5306.648 kb on + strand at 5306.648 kb on + strand at 5306.648 kb on + strand at 5306.648 kb on + strand at 5306.648 kb on + strand at 5306.648 kb on + strand at 5306.648 kb on + strand at 5306.649 kb on - strand at 5306.649 kb on - strand at 5306.649 kb on - strand at 5306.649 kb on - strand at 5306.651 kb on + strand at 5306.651 kb on + strand at 5306.651 kb on + strand at 5306.651 kb on + strand at 5306.652 kb on - strand at 5306.652 kb on - strand at 5306.652 kb on - strand at 5306.652 kb on - strand at 5306.652 kb on - strand at 5306.674 kb on + strand at 5306.675 kb on - strand at 5306.675 kb on - strand at 5306.675 kb on - strand at 5306.675 kb on - strand at 5306.675 kb on - strand at 5306.676 kb on + strand at 5306.677 kb on - strand at 5306.737 kb on + strand at 5306.737 kb on + strand at 5306.737 kb on + strand at 5306.738 kb on - strand at 5306.738 kb on - strand at 5306.738 kb on - strand at 5306.766 kb on + strand at 5306.767 kb on - strand at 5306.908 kb on + strand, within Pf6N2E2_4576 at 5306.909 kb on - strand, within Pf6N2E2_4576 at 5306.909 kb on - strand, within Pf6N2E2_4576 at 5306.912 kb on - strand, within Pf6N2E2_4576 at 5306.960 kb on - strand, within Pf6N2E2_4576 at 5306.960 kb on - strand, within Pf6N2E2_4576 at 5306.960 kb on - strand, within Pf6N2E2_4576 at 5307.031 kb on + strand, within Pf6N2E2_4576 at 5307.031 kb on + strand, within Pf6N2E2_4576 at 5307.032 kb on - strand, within Pf6N2E2_4576 at 5307.032 kb on - strand, within Pf6N2E2_4576 at 5307.168 kb on + strand, within Pf6N2E2_4576 at 5307.169 kb on - strand, within Pf6N2E2_4576 at 5307.169 kb on - strand, within Pf6N2E2_4576 at 5307.172 kb on + strand, within Pf6N2E2_4576 at 5307.172 kb on + strand, within Pf6N2E2_4576 at 5307.172 kb on + strand, within Pf6N2E2_4576 at 5307.172 kb on + strand, within Pf6N2E2_4576 at 5307.172 kb on + strand, within Pf6N2E2_4576 at 5307.172 kb on + strand, within Pf6N2E2_4576 at 5307.173 kb on - strand, within Pf6N2E2_4576 at 5307.173 kb on - strand, within Pf6N2E2_4576 at 5307.173 kb on - strand, within Pf6N2E2_4576 at 5307.173 kb on - strand, within Pf6N2E2_4576 at 5307.173 kb on - strand, within Pf6N2E2_4576 at 5307.173 kb on - strand, within Pf6N2E2_4576 at 5307.334 kb on + strand, within Pf6N2E2_4576 at 5307.341 kb on + strand, within Pf6N2E2_4576 at 5307.341 kb on + strand, within Pf6N2E2_4576 at 5307.341 kb on + strand, within Pf6N2E2_4576 at 5307.341 kb on + strand, within Pf6N2E2_4576 at 5307.342 kb on - strand, within Pf6N2E2_4576 at 5307.342 kb on - strand, within Pf6N2E2_4576 at 5307.409 kb on + strand at 5307.409 kb on + strand at 5307.410 kb on - strand at 5307.410 kb on - strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.412 kb on + strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.413 kb on - strand at 5307.448 kb on + strand at 5307.449 kb on - strand at 5307.449 kb on - strand at 5307.587 kb on + strand, within Pf6N2E2_4577 at 5307.588 kb on - strand, within Pf6N2E2_4577 at 5307.603 kb on - strand, within Pf6N2E2_4577 at 5307.603 kb on - strand, within Pf6N2E2_4577 at 5307.768 kb on - strand, within Pf6N2E2_4577 at 5307.768 kb on - strand, within Pf6N2E2_4577 at 5307.788 kb on + strand, within Pf6N2E2_4577 at 5307.789 kb on - strand, within Pf6N2E2_4577 at 5307.842 kb on + strand, within Pf6N2E2_4577 at 5307.842 kb on + strand, within Pf6N2E2_4577 at 5307.842 kb on + strand, within Pf6N2E2_4577 at 5307.842 kb on + strand, within Pf6N2E2_4577 at 5307.842 kb on + strand, within Pf6N2E2_4577 at 5307.842 kb on + strand, within Pf6N2E2_4577 at 5307.843 kb on - strand, within Pf6N2E2_4577 at 5307.843 kb on - strand, within Pf6N2E2_4577 at 5307.843 kb on - strand, within Pf6N2E2_4577 at 5307.843 kb on - strand, within Pf6N2E2_4577 at 5307.843 kb on - strand, within Pf6N2E2_4577 at 5308.073 kb on - strand, within Pf6N2E2_4577 at 5308.202 kb on + strand, within Pf6N2E2_4577 at 5308.203 kb on - strand, within Pf6N2E2_4577 at 5308.231 kb on + strand, within Pf6N2E2_4577 at 5308.231 kb on + strand, within Pf6N2E2_4577 at 5308.232 kb on - strand, within Pf6N2E2_4577 at 5308.277 kb on + strand at 5308.278 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2 remove 5,305,840 + Pf6N2E2_4575 0.45 +1.8 5,305,840 + Pf6N2E2_4575 0.45 +0.0 5,305,841 - Pf6N2E2_4575 0.45 +1.0 5,305,841 - Pf6N2E2_4575 0.45 +1.0 5,306,209 - Pf6N2E2_4575 0.71 +0.4 5,306,239 + Pf6N2E2_4575 0.73 +0.0 5,306,239 + Pf6N2E2_4575 0.73 -0.5 5,306,239 + Pf6N2E2_4575 0.73 +1.0 5,306,240 - Pf6N2E2_4575 0.73 +1.0 5,306,240 - Pf6N2E2_4575 0.73 -0.5 5,306,240 - Pf6N2E2_4575 0.73 +0.0 5,306,281 + Pf6N2E2_4575 0.76 +1.0 5,306,281 + Pf6N2E2_4575 0.76 -0.3 5,306,281 + Pf6N2E2_4575 0.76 -0.5 5,306,282 - Pf6N2E2_4575 0.76 -1.0 5,306,369 - Pf6N2E2_4575 0.82 +1.0 5,306,481 + Pf6N2E2_4575 0.90 +0.0 5,306,482 - Pf6N2E2_4575 0.90 +1.0 5,306,482 - Pf6N2E2_4575 0.90 +1.0 5,306,546 - +1.0 5,306,611 + -0.3 5,306,611 + -0.8 5,306,611 + +1.0 5,306,645 + -0.3 5,306,646 - +1.0 5,306,648 + +1.0 5,306,648 + -1.3 5,306,648 + +0.0 5,306,648 + +1.0 5,306,648 + +1.0 5,306,648 + +1.0 5,306,648 + +0.0 5,306,648 + +1.0 5,306,649 - -1.0 5,306,649 - +1.0 5,306,649 - -0.3 5,306,649 - +1.6 5,306,651 + +1.0 5,306,651 + -0.3 5,306,651 + -0.5 5,306,651 + -0.5 5,306,652 - +0.0 5,306,652 - +1.0 5,306,652 - -0.3 5,306,652 - +0.0 5,306,652 - +0.0 5,306,674 + +1.0 5,306,675 - +1.0 5,306,675 - +1.0 5,306,675 - -0.2 5,306,675 - +1.0 5,306,675 - +0.0 5,306,676 + +1.0 5,306,677 - +1.6 5,306,737 + -0.5 5,306,737 + +1.0 5,306,737 + -1.0 5,306,738 - +0.0 5,306,738 - -0.7 5,306,738 - +2.6 5,306,766 + +0.0 5,306,767 - +1.0 5,306,908 + Pf6N2E2_4576 0.18 +1.0 5,306,909 - Pf6N2E2_4576 0.18 +0.0 5,306,909 - Pf6N2E2_4576 0.18 -1.0 5,306,912 - Pf6N2E2_4576 0.18 +1.6 5,306,960 - Pf6N2E2_4576 0.26 +0.0 5,306,960 - Pf6N2E2_4576 0.26 +0.0 5,306,960 - Pf6N2E2_4576 0.26 +1.0 5,307,031 + Pf6N2E2_4576 0.36 +0.0 5,307,031 + Pf6N2E2_4576 0.36 +0.0 5,307,032 - Pf6N2E2_4576 0.37 +1.0 5,307,032 - Pf6N2E2_4576 0.37 +0.0 5,307,168 + Pf6N2E2_4576 0.57 -1.0 5,307,169 - Pf6N2E2_4576 0.57 -1.3 5,307,169 - Pf6N2E2_4576 0.57 +1.0 5,307,172 + Pf6N2E2_4576 0.58 -0.5 5,307,172 + Pf6N2E2_4576 0.58 -0.5 5,307,172 + Pf6N2E2_4576 0.58 -1.0 5,307,172 + Pf6N2E2_4576 0.58 -1.0 5,307,172 + Pf6N2E2_4576 0.58 -0.3 5,307,172 + Pf6N2E2_4576 0.58 -1.0 5,307,173 - Pf6N2E2_4576 0.58 -0.5 5,307,173 - Pf6N2E2_4576 0.58 -0.5 5,307,173 - Pf6N2E2_4576 0.58 -0.5 5,307,173 - Pf6N2E2_4576 0.58 +1.0 5,307,173 - Pf6N2E2_4576 0.58 -1.0 5,307,173 - Pf6N2E2_4576 0.58 +1.0 5,307,334 + Pf6N2E2_4576 0.82 +1.0 5,307,341 + Pf6N2E2_4576 0.83 +0.0 5,307,341 + Pf6N2E2_4576 0.83 -0.4 5,307,341 + Pf6N2E2_4576 0.83 +1.0 5,307,341 + Pf6N2E2_4576 0.83 -1.3 5,307,342 - Pf6N2E2_4576 0.83 -0.8 5,307,342 - Pf6N2E2_4576 0.83 +0.0 5,307,409 + +1.0 5,307,409 + +0.0 5,307,410 - +1.0 5,307,410 - +0.0 5,307,412 + +0.0 5,307,412 + +1.0 5,307,412 + -0.3 5,307,412 + +1.0 5,307,412 + +1.0 5,307,412 + -0.3 5,307,412 + -0.4 5,307,412 + +0.0 5,307,412 + +1.0 5,307,412 + -0.2 5,307,412 + +0.0 5,307,412 + -0.2 5,307,413 - +1.0 5,307,413 - -0.5 5,307,413 - +0.5 5,307,413 - -0.5 5,307,413 - +1.0 5,307,413 - +1.0 5,307,413 - -0.2 5,307,413 - +1.0 5,307,413 - +1.0 5,307,413 - -0.5 5,307,413 - -1.4 5,307,413 - +0.7 5,307,448 + -1.1 5,307,449 - +0.0 5,307,449 - +1.0 5,307,587 + Pf6N2E2_4577 0.12 -1.4 5,307,588 - Pf6N2E2_4577 0.12 +0.6 5,307,603 - Pf6N2E2_4577 0.14 +1.0 5,307,603 - Pf6N2E2_4577 0.14 -0.5 5,307,768 - Pf6N2E2_4577 0.33 -0.5 5,307,768 - Pf6N2E2_4577 0.33 +1.0 5,307,788 + Pf6N2E2_4577 0.35 -0.5 5,307,789 - Pf6N2E2_4577 0.36 -1.0 5,307,842 + Pf6N2E2_4577 0.42 +0.0 5,307,842 + Pf6N2E2_4577 0.42 +1.0 5,307,842 + Pf6N2E2_4577 0.42 -0.5 5,307,842 + Pf6N2E2_4577 0.42 -0.5 5,307,842 + Pf6N2E2_4577 0.42 +0.5 5,307,842 + Pf6N2E2_4577 0.42 +0.6 5,307,843 - Pf6N2E2_4577 0.42 +0.0 5,307,843 - Pf6N2E2_4577 0.42 +0.0 5,307,843 - Pf6N2E2_4577 0.42 +0.0 5,307,843 - Pf6N2E2_4577 0.42 -0.5 5,307,843 - Pf6N2E2_4577 0.42 -0.7 5,308,073 - Pf6N2E2_4577 0.69 +0.0 5,308,202 + Pf6N2E2_4577 0.84 +0.0 5,308,203 - Pf6N2E2_4577 0.84 -0.5 5,308,231 + Pf6N2E2_4577 0.87 +1.0 5,308,231 + Pf6N2E2_4577 0.87 -0.5 5,308,232 - Pf6N2E2_4577 0.87 +1.0 5,308,277 + +0.0 5,308,278 - +1.0
Or see this region's nucleotide sequence